Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BG256_RS03510 Genome accession   NZ_CP017811
Coordinates   725425..726819 (-) Length   464 a.a.
NCBI ID   WP_071162799.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NML-Hia-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 720425..731819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG256_RS03475 (BG256_03475) - 720676..720882 (-) 207 WP_013525530.1 heavy-metal-associated domain-containing protein -
  BG256_RS03480 (BG256_03480) - 720956..721162 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  BG256_RS03485 (BG256_03485) cueR 721239..721625 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  BG256_RS03490 (BG256_03490) metJ 721639..721956 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  BG256_RS03495 (BG256_03495) rho 722203..723465 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  BG256_RS03500 (BG256_03500) pilD 723519..724211 (-) 693 WP_071162797.1 prepilin peptidase Machinery gene
  BG256_RS03505 (BG256_03505) pilC 724208..725428 (-) 1221 WP_071162798.1 type II secretion system F family protein Machinery gene
  BG256_RS03510 (BG256_03510) pilB 725425..726819 (-) 1395 WP_071162799.1 GspE/PulE family protein Machinery gene
  BG256_RS03515 (BG256_03515) - 726816..727261 (-) 446 Protein_670 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BG256_RS03520 (BG256_03520) ampD 727376..727927 (+) 552 WP_071162800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BG256_RS03525 (BG256_03525) corC 728558..729457 (+) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  BG256_RS03530 (BG256_03530) lnt 729480..731009 (+) 1530 WP_080481790.1 apolipoprotein N-acyltransferase -
  BG256_RS03535 (BG256_03535) rsmE 731059..731796 (+) 738 WP_014550478.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52718.15 Da        Isoelectric Point: 5.8364

>NTDB_id=203122 BG256_RS03510 WP_071162799.1 725425..726819(-) (pilB) [Haemophilus influenzae strain NML-Hia-1]
MTSYALLHTQRVTAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPNQMQVEEQVKFYQHQETYFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRVLSQPQGLILVTGPTGSGKSISLYTALQWINTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKC
GGNLANSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDGKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=203122 BG256_RS03510 WP_071162799.1 725425..726819(-) (pilB) [Haemophilus influenzae strain NML-Hia-1]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATATTTACGATCTCGCCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTACGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGAACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCTATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGTTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGTGTGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGATAAATACGCCTGATAAACATATTATGACTGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGATATTATTATGCTGGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTCCTACATTGGCA
GCAGAATGGTTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGTATAACAGATGGAA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

97.408

99.784

0.972
  pilB Haemophilus influenzae 86-028NP

96.983

100

0.97
  pilB Glaesserella parasuis strain SC1401

56.863

98.922

0.563
  pilB Vibrio cholerae strain A1552

39.848

100

0.453
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Legionella pneumophila strain ERS1305867

39.554

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

39.752

100

0.414
  pilB Acinetobacter baylyi ADP1

38.71

100

0.414
  pilB Acinetobacter baumannii D1279779

39.501

100

0.409
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.607

100

0.379
  pilF Thermus thermophilus HB27

36.214

100

0.379

Multiple sequence alignment