Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BG256_RS03500 Genome accession   NZ_CP017811
Coordinates   723519..724211 (-) Length   230 a.a.
NCBI ID   WP_071162797.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NML-Hia-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 718519..729211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG256_RS03470 (BG256_03470) - 720396..720602 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  BG256_RS03475 (BG256_03475) - 720676..720882 (-) 207 WP_013525530.1 heavy-metal-associated domain-containing protein -
  BG256_RS03480 (BG256_03480) - 720956..721162 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  BG256_RS03485 (BG256_03485) cueR 721239..721625 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  BG256_RS03490 (BG256_03490) metJ 721639..721956 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  BG256_RS03495 (BG256_03495) rho 722203..723465 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  BG256_RS03500 (BG256_03500) pilD 723519..724211 (-) 693 WP_071162797.1 prepilin peptidase Machinery gene
  BG256_RS03505 (BG256_03505) pilC 724208..725428 (-) 1221 WP_071162798.1 type II secretion system F family protein Machinery gene
  BG256_RS03510 (BG256_03510) pilB 725425..726819 (-) 1395 WP_071162799.1 GspE/PulE family protein Machinery gene
  BG256_RS03515 (BG256_03515) - 726816..727261 (-) 446 Protein_670 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BG256_RS03520 (BG256_03520) ampD 727376..727927 (+) 552 WP_071162800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26802.86 Da        Isoelectric Point: 9.1329

>NTDB_id=203120 BG256_RS03500 WP_071162797.1 723519..724211(-) (pilD) [Haemophilus influenzae strain NML-Hia-1]
MIYFIMFLLGGILGIALWFYLSGFITHLQQEIYATYVELFPQNRSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKCYYR
KEAFGRGDYWLAMALGSFIRLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=203120 BG256_RS03500 WP_071162797.1 723519..724211(-) (pilD) [Haemophilus influenzae strain NML-Hia-1]
ATGATTTATTTCATAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACGCA
TTTGCAGCAAGAGATTTATGCGACTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTGCTAACGT
TATCTGAAAGCATAAAAAGTGCGGCTAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATGTTATTACAGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCGTTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

97.826

100

0.978
  pilD Haemophilus influenzae Rd KW20

95.217

100

0.952

Multiple sequence alignment