Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CN03_RS16185 Genome accession   NZ_CP017810
Coordinates   3512794..3514893 (-) Length   699 a.a.
NCBI ID   WP_075274332.1    Uniprot ID   -
Organism   Thalassolituus oleivorans strain K188     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3507794..3519893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN03_RS16170 (CN03_16170) - 3509699..3511147 (-) 1449 WP_075274330.1 SPOR domain-containing protein -
  CN03_RS16175 (CN03_16175) aroB 3511165..3512256 (-) 1092 WP_075274331.1 3-dehydroquinate synthase -
  CN03_RS16180 (CN03_16180) aroK 3512271..3512789 (-) 519 WP_197019469.1 shikimate kinase AroK -
  CN03_RS16185 (CN03_16185) pilQ 3512794..3514893 (-) 2100 WP_075274332.1 type IV pilus secretin PilQ Machinery gene
  CN03_RS16190 (CN03_16190) - 3514896..3515435 (-) 540 WP_015488343.1 pilus assembly protein PilP -
  CN03_RS16195 (CN03_16195) - 3515437..3516075 (-) 639 WP_025266366.1 type 4a pilus biogenesis protein PilO -
  CN03_RS16200 (CN03_16200) - 3516077..3516631 (-) 555 WP_015488345.1 PilN domain-containing protein -
  CN03_RS16205 (CN03_16205) pilM 3516631..3517728 (-) 1098 WP_015488346.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75434.92 Da        Isoelectric Point: 4.8067

>NTDB_id=203110 CN03_RS16185 WP_075274332.1 3512794..3514893(-) (pilQ) [Thalassolituus oleivorans strain K188]
MKIIKGLRQAVFAVPAMLLLMSADALAVTLKDIKFSTMAGDITEIALEFDGAPPEVSGYTIEKPARIAIDLPGVQSALQT
KRHEIGNGNARSATIVATKDRARLVVNLTQLAAYTTTANGNTLLVRIGERSEEKVFAGNTVTSASGASVSPASDMKVVNV
DFRRGELGEGQIQIMLNDARASANIRREGSKIIADLSGVRLPDVLSRRLDVTDFATPVKYVDARREDGVTRIVIEPTSDN
FEYLAYQTDKLLSINVSEPPKDQKEAQKKQFPFTGEKLSLNFQNIEVRAVLQLIADFTGLNLVASDSVQGAITLRLQNVP
WDQALDLVLKTKGLGQRQMGSVLLIAPAAEIAAREKVELEAVRQVEELAPLVTEYMQLKYAKASVLATLLTSEQGLLSER
GTAVVDERTNTLLMKDTSANLEKVREAIIMLDVPVRQVLIEARIVVANTSVGKELGVKWGGASFKDNGSNWTTAGGSQQT
LTEGSQILFDRATTGSSTQKIDLTNANIVDFGATNAAASTFAIGYQTADFLLDLELSAIETEGRAEIVSQPRVITADGQT
ASIESGTEIPYQQASSSGATNVAFKSAVLRLEVTPQITPDDRIIMDLLINQDSVGEQTSAGPSINTNAVETQVLVENGET
VVLGGIFRSEDVTTIRKTPFFGDLPLIGALFRYKSKTDTKSELLVFITPRLVKDTLSTR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=203110 CN03_RS16185 WP_075274332.1 3512794..3514893(-) (pilQ) [Thalassolituus oleivorans strain K188]
ATGAAAATAATTAAGGGCTTACGTCAGGCTGTATTCGCGGTGCCGGCAATGTTATTGCTGATGTCTGCTGATGCCTTGGC
TGTCACACTGAAAGACATAAAATTTTCAACCATGGCGGGTGATATTACCGAGATCGCGTTGGAGTTTGATGGGGCGCCGC
CAGAAGTTAGTGGTTACACCATTGAGAAGCCTGCACGTATCGCAATTGACTTACCAGGTGTGCAGAGTGCTCTACAAACC
AAGCGTCATGAAATTGGTAACGGTAATGCGCGCAGTGCGACCATTGTGGCGACTAAAGATCGTGCGCGTCTCGTAGTGAA
TTTAACTCAGTTGGCGGCTTACACAACAACAGCCAACGGCAATACCTTACTTGTTCGAATCGGAGAGCGTTCGGAAGAGA
AAGTATTCGCTGGAAACACAGTTACCAGTGCTTCAGGCGCTTCAGTTAGTCCTGCTAGCGATATGAAAGTCGTTAACGTT
GATTTCCGTCGTGGAGAGCTAGGTGAGGGTCAAATTCAGATCATGCTGAACGATGCGCGAGCGTCTGCCAACATACGTCG
TGAAGGTTCAAAAATTATTGCAGATTTAAGTGGCGTGCGTTTACCTGATGTATTAAGCCGTCGACTTGATGTCACTGATT
TCGCCACGCCCGTTAAATATGTTGATGCGCGTCGTGAAGATGGCGTAACGCGTATTGTGATTGAGCCGACTTCGGATAAC
TTTGAGTATTTGGCCTATCAAACCGATAAATTGTTATCGATCAACGTATCTGAGCCACCAAAAGATCAAAAAGAAGCACA
GAAGAAGCAATTTCCATTTACCGGTGAAAAGCTATCTCTTAACTTCCAAAATATCGAAGTTCGCGCTGTGTTGCAGTTGA
TTGCGGACTTCACTGGTTTGAACTTAGTTGCCTCAGATTCAGTGCAGGGGGCGATTACGCTGCGTTTGCAGAATGTGCCT
TGGGATCAAGCATTGGATTTAGTGCTAAAAACCAAAGGTTTAGGGCAGCGTCAAATGGGCTCTGTGCTCTTGATTGCACC
AGCGGCTGAAATTGCTGCGCGTGAAAAAGTGGAGCTCGAAGCGGTTCGTCAAGTTGAAGAACTGGCACCGTTAGTTACGG
AATACATGCAGCTTAAATATGCCAAAGCATCTGTACTGGCGACATTGTTGACGTCTGAGCAAGGTTTGTTATCAGAGCGC
GGTACTGCGGTAGTTGATGAACGTACTAATACATTGCTAATGAAAGACACTTCTGCAAACTTAGAAAAAGTACGCGAAGC
TATCATTATGCTGGACGTTCCTGTTCGCCAAGTGCTGATTGAAGCTCGCATTGTGGTGGCAAACACCAGTGTTGGTAAAG
AACTCGGCGTGAAATGGGGTGGGGCTTCGTTTAAAGACAATGGCAGCAATTGGACTACTGCTGGTGGCAGTCAGCAAACC
TTAACGGAAGGCTCGCAGATCTTGTTTGATCGCGCGACCACTGGCAGTTCTACACAAAAAATCGATTTGACCAACGCCAA
CATAGTGGATTTCGGTGCTACAAACGCAGCAGCATCAACGTTCGCGATTGGATATCAAACAGCTGATTTCTTGTTGGATC
TAGAGTTGTCGGCGATTGAAACCGAAGGTCGTGCTGAAATTGTATCCCAGCCGCGTGTGATTACAGCGGATGGTCAGACT
GCCTCTATTGAGTCAGGTACAGAAATTCCATACCAGCAAGCTAGCTCAAGTGGCGCGACTAACGTGGCCTTTAAGTCAGC
TGTTTTACGCTTGGAAGTGACTCCGCAAATTACTCCTGATGATCGAATTATCATGGATTTGCTCATTAATCAGGACTCTG
TTGGCGAGCAAACCTCAGCTGGTCCTAGTATCAATACCAATGCGGTTGAGACTCAAGTGTTAGTTGAGAATGGCGAAACA
GTAGTACTTGGTGGTATCTTCCGTTCTGAAGATGTGACCACCATCCGTAAAACACCGTTTTTTGGCGATTTACCTCTGAT
TGGTGCGTTGTTCCGCTATAAGAGTAAGACGGATACTAAGAGTGAGCTGCTTGTGTTCATTACCCCTCGTTTGGTGAAGG
ATACGCTCAGCACTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-437)

AMIN domain (AMIN)

(41-127)

Bacterial type II and III secretion system protein (Secretin)

(538-693)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.515

100

0.491
  pilQ Acinetobacter baumannii strain A118

41.918

100

0.438
  pilQ Acinetobacter baumannii D1279779

41.918

100

0.438
  comQ Acinetobacter baylyi ADP1

40.334

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

39.877

93.276

0.372
  pilQ Legionella pneumophila strain Lp02

39.877

93.276

0.372
  pilQ Neisseria gonorrhoeae strain FA1090

34.765

100

0.371

Multiple sequence alignment