Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CN03_RS16205 Genome accession   NZ_CP017810
Coordinates   3516631..3517728 (-) Length   365 a.a.
NCBI ID   WP_015488346.1    Uniprot ID   -
Organism   Thalassolituus oleivorans strain K188     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3511631..3522728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN03_RS16180 (CN03_16180) aroK 3512271..3512789 (-) 519 WP_197019469.1 shikimate kinase AroK -
  CN03_RS16185 (CN03_16185) pilQ 3512794..3514893 (-) 2100 WP_075274332.1 type IV pilus secretin PilQ Machinery gene
  CN03_RS16190 (CN03_16190) - 3514896..3515435 (-) 540 WP_015488343.1 pilus assembly protein PilP -
  CN03_RS16195 (CN03_16195) - 3515437..3516075 (-) 639 WP_025266366.1 type 4a pilus biogenesis protein PilO -
  CN03_RS16200 (CN03_16200) - 3516077..3516631 (-) 555 WP_015488345.1 PilN domain-containing protein -
  CN03_RS16205 (CN03_16205) pilM 3516631..3517728 (-) 1098 WP_015488346.1 pilus assembly protein PilM Machinery gene
  CN03_RS16210 (CN03_16210) - 3517861..3520455 (+) 2595 WP_075274333.1 penicillin-binding protein 1A -
  CN03_RS16215 (CN03_16215) - 3520518..3521921 (-) 1404 WP_223248680.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 39515.55 Da        Isoelectric Point: 4.2678

>NTDB_id=203111 CN03_RS16205 WP_015488346.1 3516631..3517728(-) (pilM) [Thalassolituus oleivorans strain K188]
MQAREFDGDFVVLASLFNKKNKALLGVDISSTSVKVLELSRQGDRYQVEAYATEPLPLNAVVEQSISNDEAVGEAIRKAI
ARSRTSAKAAAIAVAGSAVITKTVHMSATLKDDEMDFQIRAEADQYIPYPLEEVALDWEIQGPSEGLADMVDVLLAACRS
ETVDRRKDAVEYAGISVERVDVEAFCTERAFALLGAQVVGEDVETVAVLDIGATMTTLSVLHEGKSIYTREQLFGGKQLT
EDIKRRYGLSDEEAQRAKIEGGVPDDYESEVLDPFRQSVVQQVSRSLQFFYSSSQFNDVDCIVLAGGTSAIAGLADRVQE
TLGIPTVVANPFVSMTLSQKVNANLLNSDAPSLMIAAGLALRSFD

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=203111 CN03_RS16205 WP_015488346.1 3516631..3517728(-) (pilM) [Thalassolituus oleivorans strain K188]
ATGCAAGCTCGCGAATTTGATGGGGATTTTGTCGTGCTGGCTAGCCTGTTTAACAAGAAAAATAAGGCGCTTCTGGGCGT
GGACATAAGCTCAACGTCAGTCAAAGTGCTCGAGCTCAGCCGTCAAGGCGATCGCTATCAGGTGGAAGCCTATGCGACAG
AGCCTCTCCCGCTCAATGCGGTTGTAGAGCAGAGTATTAGCAATGACGAAGCGGTAGGTGAAGCAATTCGTAAAGCAATT
GCTCGTTCGCGCACGAGTGCAAAAGCAGCAGCCATTGCGGTTGCTGGCTCAGCGGTGATTACTAAAACCGTTCATATGAG
TGCGACTCTTAAAGACGATGAGATGGATTTTCAGATTCGCGCAGAGGCGGATCAGTATATTCCATACCCTCTCGAAGAGG
TCGCGCTTGATTGGGAAATTCAAGGCCCATCAGAAGGATTAGCCGATATGGTTGATGTCCTTTTGGCTGCTTGTCGCTCT
GAAACCGTTGATCGTCGTAAAGACGCAGTCGAATATGCCGGTATTTCGGTAGAGCGCGTCGACGTTGAAGCATTTTGCAC
AGAGCGTGCCTTTGCTCTCTTGGGAGCGCAGGTTGTTGGTGAAGATGTGGAGACCGTCGCAGTTCTTGATATTGGCGCGA
CCATGACCACGCTTAGTGTTCTTCATGAAGGGAAATCAATTTACACTCGCGAACAGTTGTTCGGGGGGAAGCAATTGACG
GAAGACATTAAAAGACGTTACGGCTTGTCGGATGAAGAAGCACAGCGTGCAAAAATAGAAGGTGGTGTTCCTGATGATTA
TGAATCTGAAGTCTTAGATCCATTTCGCCAGTCGGTTGTGCAGCAAGTGAGTCGTTCATTGCAATTTTTCTATTCATCAA
GTCAATTTAATGATGTCGATTGTATTGTTTTAGCAGGTGGTACGTCAGCGATTGCTGGATTGGCTGATAGAGTTCAAGAA
ACTCTGGGTATCCCTACCGTGGTGGCAAATCCTTTCGTTAGCATGACGCTGTCGCAAAAGGTAAATGCGAATTTGTTAAA
TAGTGATGCACCTTCTCTAATGATTGCGGCAGGATTGGCATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(26-364)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.714

95.89

0.515
  comM Acinetobacter nosocomialis M2

53.714

95.89

0.515
  comM Acinetobacter baylyi ADP1

50.286

95.89

0.482
  pilM Legionella pneumophila strain ERS1305867

43.099

97.26

0.419

Multiple sequence alignment