Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CN03_RS14640 Genome accession   NZ_CP017810
Coordinates   3181700..3183397 (+) Length   565 a.a.
NCBI ID   WP_075274194.1    Uniprot ID   -
Organism   Thalassolituus oleivorans strain K188     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3176700..3188397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN03_RS14615 (CN03_14615) ampE 3176706..3177581 (-) 876 WP_075274189.1 regulatory signaling modulator protein AmpE -
  CN03_RS14620 (CN03_14620) ampD 3177642..3178181 (-) 540 WP_075274190.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CN03_RS14625 (CN03_14625) - 3178319..3179662 (+) 1344 WP_075274191.1 sodium:alanine symporter family protein -
  CN03_RS14630 (CN03_14630) nadC 3179837..3180688 (+) 852 WP_075274192.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CN03_RS18725 (CN03_14635) - 3180968..3181462 (+) 495 WP_075274193.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CN03_RS14640 (CN03_14640) pilB 3181700..3183397 (+) 1698 WP_075274194.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CN03_RS14645 (CN03_14645) pilC 3183442..3184671 (+) 1230 WP_075274195.1 type II secretion system F family protein Machinery gene
  CN03_RS14650 (CN03_14650) pilD 3184676..3185563 (+) 888 WP_075274606.1 A24 family peptidase Machinery gene
  CN03_RS14655 (CN03_14655) coaE 3185603..3186202 (+) 600 WP_075274196.1 dephospho-CoA kinase -
  CN03_RS14660 (CN03_14660) yacG 3186239..3186433 (+) 195 WP_015485862.1 DNA gyrase inhibitor YacG -
  CN03_RS14665 (CN03_14665) - 3186489..3187670 (-) 1182 WP_075274197.1 two-component system response regulator -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61703.80 Da        Isoelectric Point: 4.9634

>NTDB_id=203103 CN03_RS14640 WP_075274194.1 3181700..3183397(+) (pilB) [Thalassolituus oleivorans strain K188]
MALLTGLPRRLVEQQLLNQAQAEEILASCKKEKQSFVTLAVAKKLVKAKELAVLASEEFGLPLLDLATFDGELMPKELVS
SELIDKHRVLPLFVRGNRLFVAQSDPSQMVAIDEIKFNTGMTVEAILVEEDKLSVAVDRFLSANDNTLGDLDSALDDIDL
DDPNARDKEDEQNSNAGGDDAPIVRFVNKILLDAIKSGASDVHFEPYEKRYRVRYRTDGILLEVSSPPVNLAGKIAARLK
VMSQMDISERRLPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEPEQKDLYLSALAKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFAAALRSFLRQDPDVVMVGEIRDLE
TAQIAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPSFNIATSVSLIIAQRLARRLCVQCKVPAEIPEHTLKEVGFTDE
LLADAEIFKPVGCEHCNHGYKGRVGIYEVVRVTDAISRIIMEGGNSIEISDAARKEGFADLRTSALKKVAQGVTSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=203103 CN03_RS14640 WP_075274194.1 3181700..3183397(+) (pilB) [Thalassolituus oleivorans strain K188]
ATGGCGTTATTGACCGGCCTCCCTCGTCGTCTCGTTGAGCAACAACTTCTCAACCAAGCGCAAGCCGAAGAGATACTCGC
CAGTTGTAAAAAAGAAAAACAAAGCTTTGTCACACTGGCTGTCGCGAAAAAATTAGTTAAAGCAAAAGAGCTTGCGGTGT
TAGCCTCTGAAGAATTCGGCTTACCGCTATTAGACCTAGCAACATTTGACGGCGAATTGATGCCAAAAGAACTGGTCAGT
TCTGAACTCATCGACAAGCACCGTGTGCTACCACTATTTGTTCGCGGTAACCGATTATTTGTAGCGCAAAGCGATCCATC
GCAAATGGTCGCTATCGACGAAATCAAGTTCAATACCGGCATGACGGTAGAAGCGATACTCGTTGAAGAAGATAAACTCA
GCGTTGCCGTTGATCGTTTTTTATCCGCCAACGACAATACCTTGGGCGATCTCGATAGCGCGCTCGACGACATTGATTTA
GATGACCCGAACGCCCGAGACAAAGAGGATGAACAAAATAGCAACGCAGGAGGCGATGACGCTCCTATCGTTCGCTTTGT
AAATAAAATTTTATTAGACGCAATTAAATCCGGAGCATCCGATGTTCACTTTGAGCCCTACGAAAAACGCTACCGTGTAC
GGTATCGTACCGATGGTATTCTATTAGAGGTCTCCAGCCCTCCGGTGAATTTAGCTGGTAAAATTGCGGCACGCCTAAAA
GTAATGTCGCAAATGGATATTTCTGAGCGCCGTCTTCCCCAAGATGGTCGTATCAAGATGAAGCTTTCCAAAACGAAGGC
CATCGACTTCCGCGTCAATACTTTACCCACGTTGTGGGGCGAAAAAATCGTACTACGTATTCTTGATCCGTCTTCAGCTA
AGCTAGGCATTGATGCTCTAGGCTACGAGCCAGAGCAAAAAGATTTGTACTTAAGTGCCTTGGCGAAACCACAAGGTATG
ATCTTAGTGACCGGACCTACCGGTTCTGGCAAGACAGTTTCGCTCTATACAGGCCTGAATATTCTCAACGTGGAAGGCAC
CAATATTTCCACGGCAGAAGACCCCGTTGAAATTAACTTGGAAGGTATCAACCAAGTTAACGTGAATGCCCGTGTCGGTC
TCGACTTTGCTGCCGCGCTGCGATCCTTCTTACGCCAAGACCCGGATGTAGTCATGGTGGGTGAGATTCGCGATTTGGAA
ACCGCTCAGATAGCAATCAAAGCCGCACAAACGGGTCACTTAGTGCTGTCTACTCTACACACTAATAGTGCGGCTGAAAC
ACTGACCCGTTTGGCCAATATGGGGGTTCCGTCGTTTAATATTGCAACTTCAGTAAGCCTAATTATTGCCCAGCGTTTAG
CTCGTCGCCTATGCGTGCAGTGTAAAGTGCCCGCGGAAATCCCAGAGCACACATTAAAAGAAGTGGGCTTTACCGATGAA
TTGCTTGCAGACGCCGAAATATTTAAACCGGTAGGTTGTGAACACTGCAACCATGGCTACAAAGGCCGCGTCGGTATATA
TGAAGTCGTTCGCGTTACGGATGCAATATCGCGGATCATCATGGAAGGTGGTAACTCCATTGAAATATCGGATGCTGCTC
GAAAAGAAGGATTTGCCGATTTGCGTACTTCGGCGCTTAAGAAAGTCGCTCAAGGCGTCACTAGCCTAGAAGAAGCAAAC
CGTGTGACAACCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.832

100

0.628
  pilB Acinetobacter baylyi ADP1

62.257

100

0.625
  pilB Legionella pneumophila strain ERS1305867

54.816

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

99.469

0.513
  pilB Vibrio cholerae strain A1552

50.617

100

0.508
  pilB Vibrio campbellii strain DS40M4

50.177

100

0.503
  pilF Neisseria gonorrhoeae MS11

50.177

99.823

0.501
  pilB/pilB1 Synechocystis sp. PCC 6803

40.837

88.85

0.363
  pilF Thermus thermophilus HB27

36.283

100

0.363

Multiple sequence alignment