Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BFW41_RS02240 Genome accession   NZ_CP016990
Coordinates   476944..478650 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain ZYAH75     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471944..483650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFW41_RS02210 (BFW41_02160) mutT 472620..473033 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  BFW41_RS02215 (BFW41_02165) yacG 473094..473288 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  BFW41_RS02220 (BFW41_02170) zapD 473298..474020 (-) 723 WP_024945431.1 cell division protein ZapD -
  BFW41_RS02225 (BFW41_02175) coaE 474058..474672 (-) 615 WP_118880265.1 dephospho-CoA kinase -
  BFW41_RS02230 (BFW41_02180) pilD 474692..475564 (-) 873 WP_118880267.1 A24 family peptidase Machinery gene
  BFW41_RS02235 (BFW41_02185) pilC 475587..476828 (-) 1242 WP_118880269.1 type II secretion system F family protein Machinery gene
  BFW41_RS02240 (BFW41_02190) pilB 476944..478650 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  BFW41_RS02245 - 478657..479073 (-) 417 WP_118882219.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BFW41_RS02255 (BFW41_02195) nadC 479396..480253 (-) 858 WP_118880271.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BFW41_RS02260 (BFW41_02200) - 480259..480729 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  BFW41_RS02265 (BFW41_02205) ampD 480877..481449 (+) 573 WP_118880273.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BFW41_RS02270 (BFW41_02210) pdhR 481725..482489 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=194376 BFW41_RS02240 WP_029302067.1 476944..478650(-) (pilB) [Aeromonas hydrophila strain ZYAH75]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=194376 BFW41_RS02240 WP_029302067.1 476944..478650(-) (pilB) [Aeromonas hydrophila strain ZYAH75]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAATGAAATACTCGACAGCAAGGCACTGG
CTGACTTCTGTGAACTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTCCCTATCTACACCCAAGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAAGATGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAAAGCGTCAATACGGCCGACGATGACGCACCGATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACTCCACCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTCTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CAGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAAGCGCAATACCTGCGTGCCCTCTCGAAGCCTCAG
GGTATGATCTTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCCCTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACAGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGGCAAGACCCCGATGTGGTCATGGTAGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTACTATCCACCCTGCACACAAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGCACCTCCGGCCTAGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment