Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BEN76_RS14885 Genome accession   NZ_CP016896
Coordinates   3144307..3146022 (-) Length   571 a.a.
NCBI ID   WP_076033613.1    Uniprot ID   A0A1P8EN48
Organism   Acinetobacter soli strain GFJ2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3139307..3151022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN76_RS14855 (BEN76_14855) - 3139477..3139803 (+) 327 WP_004932453.1 pyrimidine/purine nucleoside phosphorylase -
  BEN76_RS14860 (BEN76_14860) rlmB 3139906..3140652 (+) 747 WP_004932455.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BEN76_RS14865 (BEN76_14865) - 3140690..3141619 (+) 930 WP_004932459.1 DMT family transporter -
  BEN76_RS14870 (BEN76_14870) coaE 3141595..3142194 (-) 600 WP_076033404.1 dephospho-CoA kinase -
  BEN76_RS14875 (BEN76_14875) pilD 3142197..3143057 (-) 861 WP_025095300.1 prepilin peptidase Machinery gene
  BEN76_RS14880 (BEN76_14880) pilC 3143057..3144283 (-) 1227 WP_076033405.1 type II secretion system F family protein Machinery gene
  BEN76_RS14885 (BEN76_14885) pilB 3144307..3146022 (-) 1716 WP_076033613.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BEN76_RS14890 (BEN76_14890) tpiA 3146286..3147083 (+) 798 WP_004932474.1 triose-phosphate isomerase -
  BEN76_RS14895 (BEN76_14895) secG 3147093..3147422 (+) 330 WP_004932477.1 preprotein translocase subunit SecG -
  BEN76_RS14915 (BEN76_14915) rimP 3148499..3149023 (+) 525 WP_004932478.1 ribosome maturation factor RimP -
  BEN76_RS14920 (BEN76_14920) nusA 3149059..3150543 (+) 1485 WP_004943981.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 64166.78 Da        Isoelectric Point: 5.1748

>NTDB_id=193547 BEN76_RS14885 WP_076033613.1 3144307..3146022(-) (pilB) [Acinetobacter soli strain GFJ2]
MSLVTASPKFSGFIRRLVEEGHIDAHDMQMAQESAKKNKQDIVSYLIDHYAITPITIAETISVEFGEPLFDLDVFDPALI
LKDRIDEKLIRKYRVMPLVHRGHVLYVATCNPTNLEAMDAIRFNSKLKVEPIIVEHDKLERLLNEYFPEDSRFNFDTEDF
DLDLEVNTPASDDDDDDEISKDEAPVVKYINKLLIDAIRMNASDLHFEPYEKMYRVRYRVDGVLRLIATPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKALFTEALNRP
QGMLLITGPTGSGKTVSMYTGLNILNTEHANISTAEDPVEINLEGINQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRSMGVATFNIATSVNLVIAQRLARRLCNQCKRRIDVPRASLLEMGF
TETDLDAPDFQIYEPVGCKECREGYKGRVGIYEVMKITPEISRIIMQDGNALEIAAAAEQLGFSNLRRSGLKKVMQGVTS
LQEVNRVTNEE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=193547 BEN76_RS14885 WP_076033613.1 3144307..3146022(-) (pilB) [Acinetobacter soli strain GFJ2]
ATGTCATTAGTTACCGCATCACCTAAATTTTCAGGATTCATTCGGCGCCTTGTCGAAGAAGGTCATATTGATGCACACGA
CATGCAAATGGCTCAAGAGAGCGCCAAAAAAAACAAGCAAGATATTGTTTCTTATTTGATCGACCATTATGCCATTACCC
CGATCACCATTGCTGAAACCATCTCGGTCGAGTTTGGCGAACCGCTATTTGATCTGGATGTTTTTGATCCTGCGCTGATT
CTCAAAGATCGTATCGATGAAAAGCTGATTCGCAAGTATCGTGTAATGCCGTTGGTTCATCGTGGGCATGTGCTCTATGT
CGCGACCTGCAATCCGACCAATCTTGAAGCGATGGATGCCATCCGTTTTAACAGCAAACTCAAAGTCGAGCCGATTATTG
TAGAACACGATAAGCTTGAGCGACTGTTAAACGAATATTTCCCCGAAGATAGCCGTTTTAACTTTGACACCGAAGATTTT
GATCTGGATCTGGAGGTGAATACCCCCGCTTCAGATGACGACGACGATGACGAAATCAGTAAAGATGAAGCCCCGGTGGT
CAAATACATCAATAAATTACTGATTGATGCCATTCGTATGAATGCATCAGACTTACATTTTGAACCTTATGAAAAAATGT
ATCGGGTCCGTTATCGGGTCGATGGTGTACTTCGTTTAATTGCAACACCACCCTTACAACTGGCTACCCGACTGGCCTCG
CGTTTGAAAGTGATGTCTCAAATGGATATTTCGGAAAAGCGTGTTCCGCAAGATGGTCGTATTAAACTGAAACTGTCGAA
AACCAAAACCATCGATTTTCGGGTCAACTCCCTACCGACCCTATTTGGTGAAAAGATTGTACTGCGGATTCTGGATCCTG
CGAGCGCGATGCTGGGTATCGATGCACTGGGCTACGAGCCTGAGCAAAAAGCATTGTTTACCGAAGCGCTGAATCGACCA
CAAGGCATGCTGCTGATTACCGGCCCTACAGGCTCGGGGAAAACCGTATCGATGTATACCGGCTTAAATATTCTCAATAC
CGAACATGCCAATATTTCTACCGCCGAAGATCCAGTCGAGATTAATCTTGAAGGTATTAATCAGGTCAATGTAAATCCCA
AAGTCGGCCTGACCTTTGCTGCGGCATTACGCTCTTTTTTACGTCAAGACCCTGATATCATTATGGTGGGGGAAATTCGT
GATTTAGAAACCGCAGAGATTGCCATTAAAGCGGCTCAAACCGGTCACTTGGTGATGTCGACACTGCATACCAATAACGC
TGCCGAAACACTTACACGCCTGAGAAGCATGGGGGTGGCGACCTTTAATATCGCCACCTCGGTCAATCTGGTGATTGCGC
AGCGCCTTGCCAGACGCCTGTGTAATCAGTGTAAACGGCGTATCGACGTGCCACGTGCCAGTTTACTCGAAATGGGATTT
ACCGAAACCGATTTAGATGCTCCAGACTTTCAGATCTATGAACCGGTGGGGTGTAAAGAATGTCGGGAGGGCTACAAAGG
ACGTGTGGGCATTTATGAAGTCATGAAAATTACACCAGAAATCTCAAGAATTATTATGCAAGACGGCAATGCACTTGAAA
TTGCAGCAGCCGCTGAACAGCTTGGATTTAGCAACTTACGCCGTTCTGGCTTAAAAAAAGTGATGCAGGGGGTCACATCG
TTACAGGAAGTCAATCGTGTGACGAATGAAGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8EN48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

86.316

99.825

0.862
  pilB Acinetobacter baumannii D1279779

77.368

99.825

0.772
  pilB Legionella pneumophila strain ERS1305867

51.304

100

0.517
  pilF Neisseria gonorrhoeae MS11

49.644

98.424

0.489
  pilB Vibrio cholerae strain A1552

51.644

90.543

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

49.536

94.396

0.468
  pilB Vibrio campbellii strain DS40M4

50.097

90.543

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

37.77

97.373

0.368

Multiple sequence alignment