Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IEC33019_RS27905 Genome accession   NZ_CP016634
Coordinates   4790502..4791776 (-) Length   424 a.a.
NCBI ID   WP_070091953.1    Uniprot ID   A0A1B2FCF6
Organism   Pseudomonas putida strain IEC33019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4785502..4796776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEC33019_RS22335 (IEC33019_4344) - 4787143..4788768 (-) 1626 WP_070091950.1 SPOR domain-containing protein -
  IEC33019_RS22340 (IEC33019_4345) aroB 4788784..4789881 (-) 1098 WP_070091951.1 3-dehydroquinate synthase -
  IEC33019_RS22345 (IEC33019_4346) aroK 4789979..4790497 (-) 519 WP_070091952.1 shikimate kinase AroK -
  IEC33019_RS27905 (IEC33019_4347) pilQ 4790502..4791776 (-) 1275 WP_070091953.1 type IV pilus secretin PilQ Machinery gene
  IEC33019_RS27910 - 4791817..4792716 (-) 900 Protein_4294 pilus assembly protein PilP -
  IEC33019_RS22360 (IEC33019_4349) - 4792707..4793243 (-) 537 WP_070092028.1 PilN domain-containing protein -
  IEC33019_RS22365 (IEC33019_4350) pilM 4793243..4794148 (-) 906 WP_070091955.1 type IV pilus biogenesis protein PilM -
  IEC33019_RS22370 (IEC33019_4351) - 4794309..4796762 (+) 2454 WP_070091956.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 45899.58 Da        Isoelectric Point: 4.9338

>NTDB_id=189886 IEC33019_RS27905 WP_070091953.1 4790502..4791776(-) (pilQ) [Pseudomonas putida strain IEC33019]
MTLNNLKVGLVAAMLSSQAATAMPYRGEPLSLNFQDIEVRAALQVLADHAGINLVASDTVQGSLTLRLEDVPWDQALDLV
LRSKGLARREEGNVLVVAPVAELAEQFREARSGQALDAQLQPMRRELLPIHHAKAADLAELLLAALADEGILTGRGSLSV
DPRTNTLVAYQPADRLAELRRLVGQLDVPVRQVLIEARIVEANVDYEKSLGVRWGSPLQGDTARLGEDLFVDLGVERATS
GIGLGVLRGGTLLDLELSAMEKSGNGEIISQPKVVTADKETARIIKGTEVPYQETSKSGATSISFREASLSLEVTPQITP
DGKVVMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFLGDLPYVGRLFRRDVLQ
EKKSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=189886 IEC33019_RS27905 WP_070091953.1 4790502..4791776(-) (pilQ) [Pseudomonas putida strain IEC33019]
ATGACGCTGAACAATCTGAAGGTCGGCCTGGTGGCGGCGATGCTGTCGAGCCAGGCCGCGACAGCGATGCCCTATCGGGG
CGAGCCGCTGTCCCTGAACTTCCAGGACATCGAAGTACGCGCAGCGCTGCAGGTGCTGGCCGATCATGCCGGTATCAACC
TGGTGGCGAGCGACACAGTGCAAGGCAGCCTTACCTTGCGCTTGGAGGACGTGCCCTGGGATCAGGCCTTGGACCTGGTG
TTGCGCAGCAAAGGGCTGGCCCGGCGCGAGGAGGGCAACGTGCTTGTGGTCGCGCCGGTAGCGGAGCTGGCGGAGCAGTT
TCGTGAAGCGCGGTCTGGGCAGGCGCTGGATGCGCAATTGCAGCCCATGCGGCGTGAGCTGCTGCCGATCCATCACGCCA
AGGCGGCGGACTTGGCCGAATTGCTGTTGGCCGCCCTCGCCGACGAGGGCATTCTCACCGGGCGCGGTAGCCTGAGTGTC
GATCCGCGTACCAACACCCTGGTGGCGTACCAGCCGGCTGATCGCCTTGCCGAATTGCGGCGGTTGGTGGGGCAGTTGGA
CGTGCCGGTACGCCAGGTGCTGATCGAGGCGCGGATCGTCGAGGCCAACGTCGATTACGAAAAAAGCCTCGGCGTGCGCT
GGGGTTCGCCGCTACAGGGCGACACCGCCCGTCTGGGTGAGGACTTGTTCGTCGATCTGGGTGTGGAGCGGGCGACGTCC
GGCATCGGGCTCGGTGTGCTGCGTGGCGGGACACTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAATGGCGA
GATCATCTCCCAGCCCAAGGTGGTCACGGCTGACAAGGAGACCGCGCGGATCATCAAGGGGACCGAAGTGCCCTACCAGG
AAACCAGCAAGAGTGGCGCGACCTCCATCTCGTTTCGCGAAGCGTCGCTGTCGCTCGAGGTGACGCCGCAGATCACGCCC
GATGGCAAGGTCGTGATGGCTGTCAGGGTGACCAAGGACGAGCCTGATTACGTCAATGCACTGAACAACGTCCCGCCCAT
CCGCAAGAACGAGGTCAACGCCAAGGTGCGGGTGGCGGACGGTGAGACGATCGTCATCGGTGGCGTCTACTCGACCACGC
AAAACAATGTGGTCGACAAGGTGCCATTTTTAGGCGATCTGCCGTATGTTGGCAGGCTGTTTCGACGCGATGTATTACAA
GAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(259-415)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B2FCF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

60.092

100

0.618
  comQ Acinetobacter baylyi ADP1

42.984

100

0.455
  pilQ Acinetobacter baumannii D1279779

41.485

100

0.448
  pilQ Acinetobacter baumannii strain A118

41.485

100

0.448
  pilQ Legionella pneumophila strain ERS1305867

43.78

98.585

0.432
  pilQ Legionella pneumophila strain Lp02

43.78

98.585

0.432
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.453

100

0.425
  pilQ Vibrio cholerae strain A1552

42.453

100

0.425
  pilQ Vibrio campbellii strain DS40M4

39.414

100

0.413

Multiple sequence alignment