Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   B9J09_RS10820 Genome accession   NZ_CP020870
Coordinates   2246854..2247300 (+) Length   148 a.a.
NCBI ID   WP_010894960.1    Uniprot ID   Q9PAH9
Organism   Xylella fastidiosa subsp. pauca strain De Donno     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2241854..2252300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9J09_RS10800 (B9J09_10800) - 2242105..2243442 (+) 1338 WP_023908103.1 HAMP domain-containing sensor histidine kinase -
  B9J09_RS10805 (B9J09_10805) coaE 2243709..2244332 (-) 624 WP_046418843.1 dephospho-CoA kinase -
  B9J09_RS10810 (B9J09_10810) pilD 2244344..2245207 (-) 864 WP_010894958.1 A24 family peptidase Machinery gene
  B9J09_RS10815 (B9J09_10815) - 2245214..2246405 (-) 1192 Protein_2023 type II secretion system F family protein -
  B9J09_RS10820 (B9J09_10820) pilA/pilAI 2246854..2247300 (+) 447 WP_010894960.1 pilin Machinery gene
  B9J09_RS10830 (B9J09_10830) pilA 2248596..2249033 (+) 438 WP_046418469.1 pilin Machinery gene
  B9J09_RS10840 (B9J09_10840) pilB 2249871..2251604 (+) 1734 WP_046418464.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15363.49 Da        Isoelectric Point: 8.4988

>NTDB_id=189723 B9J09_RS10820 WP_010894960.1 2246854..2247300(+) (pilA/pilAI) [Xylella fastidiosa subsp. pauca strain De Donno]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKSTSNASDIGLRTDTTRCGI
TVKVDAAGTANITCKVKGNSQVNDKTIAWDRTSDNSAGTNGVNNGGVWTCSSTVTSDALRPSGCIASK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=189723 B9J09_RS10820 WP_010894960.1 2246854..2247300(+) (pilA/pilAI) [Xylella fastidiosa subsp. pauca strain De Donno]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTCCTAGCAGCCATCGCGCT
GCCTATGTACCAAAATTATGTCGCCAGATCCCAACTCACCGCGGCACTGGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGAGTACATCCAACGCGAGTGATATTGGTCTGCGTACCGACACCACTCGTTGTGGGATT
ACCGTTAAAGTCGATGCCGCTGGCACTGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAATGATAAAACCAT
CGCTTGGGATCGTACCTCCGATAACTCTGCAGGCACCAATGGTGTCAATAATGGAGGTGTGTGGACCTGCTCCTCCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATTGCCTCTAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9PAH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.857

99.324

0.426
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA Vibrio cholerae strain A1552

38.71

100

0.405
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.71

100

0.405
  pilA Vibrio cholerae C6706

38.71

100

0.405
  pilA Acinetobacter baumannii strain A118

39.333

100

0.399