Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BCY75_RS05625 Genome accession   NZ_CP016602
Coordinates   1119907..1120782 (+) Length   291 a.a.
NCBI ID   WP_065825482.1    Uniprot ID   -
Organism   Latilactobacillus curvatus strain WiKim52     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1114907..1125782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCY75_RS05585 (BCY75_05585) - 1115012..1115839 (-) 828 WP_054644033.1 LysR family transcriptional regulator -
  BCY75_RS05590 (BCY75_05590) - 1115948..1116961 (+) 1014 WP_004265264.1 YeiH family protein -
  BCY75_RS05595 (BCY75_05595) - 1117052..1117291 (-) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  BCY75_RS05600 (BCY75_05600) - 1117615..1117821 (+) 207 WP_065825481.1 hypothetical protein -
  BCY75_RS05610 (BCY75_05610) - 1118002..1118379 (-) 378 WP_056965888.1 hypothetical protein -
  BCY75_RS05615 (BCY75_05615) - 1118482..1118982 (+) 501 WP_056965890.1 VanZ family protein -
  BCY75_RS05620 (BCY75_05620) - 1119072..1119803 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BCY75_RS05625 (BCY75_05625) comGA 1119907..1120782 (+) 876 WP_065825482.1 competence type IV pilus ATPase ComGA Machinery gene
  BCY75_RS05630 (BCY75_05630) comGB 1120772..1121773 (+) 1002 WP_004270906.1 type II secretion system F family protein Machinery gene
  BCY75_RS05635 (BCY75_05635) - 1121826..1123250 (-) 1425 WP_065825483.1 recombinase family protein -
  BCY75_RS05640 (BCY75_05640) - 1123366..1123677 (-) 312 WP_065825484.1 hypothetical protein -
  BCY75_RS05645 (BCY75_05645) - 1123696..1123983 (-) 288 WP_065825485.1 BRCT domain-containing protein -
  BCY75_RS05655 (BCY75_05655) - 1124142..1124915 (-) 774 WP_065825486.1 DUF1828 domain-containing protein -
  BCY75_RS05660 (BCY75_05660) - 1124969..1125397 (-) 429 WP_065825926.1 DUF6978 family protein -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32072.87 Da        Isoelectric Point: 5.5654

>NTDB_id=189554 BCY75_RS05625 WP_065825482.1 1119907..1120782(+) (comGA) [Latilactobacillus curvatus strain WiKim52]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYATLLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVDGHNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=189554 BCY75_RS05625 WP_065825482.1 1119907..1120782(+) (comGA) [Latilactobacillus curvatus strain WiKim52]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCTTTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAACATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCTGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGACGGCCACAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.015

93.471

0.402
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.593

92.784

0.395
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.573

91.753

0.381
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.194

93.814

0.368

Multiple sequence alignment