Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BA953_RS11415 Genome accession   NZ_CP016556
Coordinates   2467288..2468934 (-) Length   548 a.a.
NCBI ID   WP_065741170.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain 58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2462288..2473934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA953_RS11385 (BA953_11385) rplS 2462917..2463270 (+) 354 WP_006958143.1 50S ribosomal protein L19 -
  BA953_RS11390 (BA953_11390) yacG 2463516..2463710 (-) 195 WP_019277521.1 DNA gyrase inhibitor YacG -
  BA953_RS11395 (BA953_11395) zapD 2463762..2464502 (-) 741 WP_006958141.1 cell division protein ZapD -
  BA953_RS11400 (BA953_11400) coaE 2464536..2465144 (-) 609 WP_065741167.1 dephospho-CoA kinase -
  BA953_RS11405 (BA953_11405) pilD 2465146..2466015 (-) 870 WP_065741168.1 A24 family peptidase Machinery gene
  BA953_RS11410 (BA953_11410) - 2466065..2467279 (-) 1215 WP_065741169.1 type II secretion system F family protein -
  BA953_RS11415 (BA953_11415) pilB 2467288..2468934 (-) 1647 WP_065741170.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BA953_RS11420 (BA953_11420) - 2468937..2469377 (-) 441 WP_031343346.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BA953_RS11425 (BA953_11425) nadC 2469605..2470492 (-) 888 WP_065741171.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BA953_RS11430 (BA953_11430) ampD 2470624..2471169 (+) 546 WP_006958131.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BA953_RS11435 (BA953_11435) fldB 2471296..2471817 (-) 522 WP_029236292.1 flavodoxin FldB -
  BA953_RS11440 (BA953_11440) xerD 2471981..2472877 (+) 897 WP_031343348.1 site-specific tyrosine recombinase XerD -
  BA953_RS11445 (BA953_11445) - 2472917..2473669 (+) 753 WP_065741172.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 60684.11 Da        Isoelectric Point: 4.7500

>NTDB_id=189167 BA953_RS11415 WP_065741170.1 2467288..2468934(-) (pilB) [Vibrio coralliilyticus strain 58]
MSELPFLLKKANKISEEQALQAISATETRQIEAYSALLEISDLTSKQLTAAISELYALPIVSLDDFDYRTCCEQLGIGDL
IRSHTALPLEVERQHLTLALADPSISEIEQEFRFATGLAIKLVLAELPTLARAINNLYGAQSASDSLGYKEISVLDLEQL
VEAEEHELIDSEDLNHDSSPISRYIQQILLDAVRKQASDIHFEPHESWFRVRFRCDGLLVEVQRPPQHLCQRLTARLKIL
AKLDIAERRLPQDGRITLNVTQTDSIDIRVSTLPTIWGEKIVLRLLDNRMTSFDLSSLGYNHIQSESYRQALTKPQGLIL
ITGPTGSGKTLSLYSGLNIINQNEINIATAEDPVEIHLTGINQVQIQPQIGFDFAQALRAFLRQDPDVVMVGEIRDRETA
EIATKAAQTGHLVLSTLHTNSAAEAILRLQNMGVEAFNLAASLSLIIAQRLVRCLCPLCKRPTKVQTGQYEASSEGCQDC
NQGYSGRTGIYEVLTVTPEIAQAINDGATANQIEDLAIQQGMQTLPHSGLEKLAQGITSQAELQRMLF

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=189167 BA953_RS11415 WP_065741170.1 2467288..2468934(-) (pilB) [Vibrio coralliilyticus strain 58]
ATGAGTGAACTGCCCTTCTTGCTAAAAAAAGCCAATAAAATTAGTGAAGAACAAGCCCTGCAAGCAATATCAGCTACAGA
AACCCGACAAATTGAGGCGTATTCTGCTCTACTAGAGATTAGTGACCTGACATCCAAACAGCTGACAGCAGCTATCAGTG
AGCTCTATGCTCTCCCCATCGTGTCGTTAGATGACTTCGATTATCGAACTTGTTGCGAGCAGCTCGGCATTGGCGACTTA
ATTCGCAGCCACACGGCACTGCCACTAGAAGTAGAGCGACAACATCTTACTCTAGCGCTCGCCGATCCTTCCATTTCCGA
GATAGAACAAGAATTTCGTTTTGCAACTGGCCTAGCAATCAAACTCGTATTGGCCGAGTTACCGACTTTAGCCCGTGCGA
TAAACAACCTATATGGAGCTCAAAGCGCCAGTGACTCTTTAGGCTACAAAGAGATTAGCGTCCTAGACCTTGAACAATTA
GTCGAAGCTGAAGAACATGAATTAATAGACAGTGAGGATCTCAATCACGATAGCTCGCCAATCAGTCGCTATATCCAGCA
GATACTGCTGGATGCCGTACGTAAGCAAGCTTCTGATATCCATTTTGAACCTCATGAATCATGGTTTCGCGTCCGGTTTC
GTTGTGATGGCTTGCTCGTTGAAGTGCAGCGACCTCCTCAGCACCTATGCCAAAGGTTAACCGCCAGACTCAAAATCCTC
GCTAAGCTGGACATCGCCGAGCGCAGACTCCCGCAAGATGGTCGTATCACTCTCAATGTGACTCAAACTGATTCTATCGA
CATACGTGTATCGACTCTGCCTACCATATGGGGTGAAAAAATTGTCCTTAGATTGCTCGACAACAGAATGACCAGCTTTG
ACTTAAGCAGCCTTGGCTACAATCACATTCAAAGCGAAAGCTATCGTCAGGCGCTAACCAAGCCTCAGGGACTAATTCTA
ATCACTGGCCCCACGGGAAGCGGTAAGACTCTGTCTTTATACAGTGGACTTAACATCATCAATCAGAATGAGATCAACAT
CGCGACGGCAGAGGACCCCGTAGAGATTCACCTAACAGGAATCAATCAGGTGCAAATTCAACCTCAAATCGGCTTTGATT
TCGCTCAGGCACTCAGAGCCTTTCTACGTCAGGATCCCGATGTGGTTATGGTGGGTGAAATTCGCGATCGAGAAACTGCC
GAGATAGCCACCAAAGCGGCTCAAACAGGCCATTTAGTCCTTTCAACACTCCATACCAACTCAGCAGCAGAAGCTATTCT
TCGCCTCCAGAATATGGGAGTAGAGGCTTTCAACCTTGCTGCTTCTCTTAGCCTCATCATTGCTCAGCGTTTGGTCAGGT
GCCTATGCCCTCTATGCAAGCGCCCTACAAAAGTCCAGACAGGTCAATATGAGGCTTCTTCTGAAGGTTGTCAGGACTGC
AACCAAGGCTACTCAGGACGAACTGGTATTTATGAAGTACTCACAGTGACACCTGAAATTGCGCAAGCTATTAACGACGG
CGCCACAGCTAACCAGATTGAGGACTTAGCGATACAACAAGGCATGCAAACATTACCGCACTCGGGCTTAGAAAAATTGG
CCCAAGGTATCACCAGTCAGGCAGAACTGCAGCGAATGCTGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

56.786

100

0.58
  pilB Vibrio parahaemolyticus RIMD 2210633

56.631

100

0.577
  pilB Vibrio campbellii strain DS40M4

56.172

100

0.573
  pilB Acinetobacter baumannii D1279779

44.547

98.723

0.44
  pilB Legionella pneumophila strain ERS1305867

46.257

95.073

0.44
  pilB Acinetobacter baylyi ADP1

45.817

95.985

0.44
  pilF Neisseria gonorrhoeae MS11

42.035

95.073

0.4
  pilB Haemophilus influenzae Rd KW20

44.017

85.401

0.376
  pilB Haemophilus influenzae 86-028NP

43.376

85.401

0.37
  pilF Thermus thermophilus HB27

41.031

88.504

0.363

Multiple sequence alignment