Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BSY239_RS15050 Genome accession   NZ_CP016449
Coordinates   3215731..3217464 (+) Length   577 a.a.
NCBI ID   WP_069047505.1    Uniprot ID   -
Organism   Hydrogenophaga sp. RAC07     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3210731..3222464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSY239_RS15020 (BSY239_3043) proB 3211259..3212431 (-) 1173 WP_069047502.1 glutamate 5-kinase -
  BSY239_RS15025 (BSY239_3044) obgE 3212443..3213513 (-) 1071 WP_069047503.1 GTPase ObgE -
  BSY239_RS15030 (BSY239_3045) rpmA 3213654..3213911 (-) 258 WP_056273119.1 50S ribosomal protein L27 -
  BSY239_RS15035 (BSY239_3046) rplU 3213925..3214236 (-) 312 WP_069047504.1 50S ribosomal protein L21 -
  BSY239_RS15040 (BSY239_3047) - 3214504..3215433 (+) 930 WP_083240201.1 polyprenyl synthetase family protein -
  BSY239_RS15050 (BSY239_3049) pilB 3215731..3217464 (+) 1734 WP_069047505.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BSY239_RS15055 (BSY239_3050) pilC 3217497..3218714 (+) 1218 WP_069047506.1 type II secretion system F family protein Machinery gene
  BSY239_RS15060 (BSY239_3051) pilD 3218714..3219577 (+) 864 WP_069047507.1 A24 family peptidase Machinery gene
  BSY239_RS15065 (BSY239_3052) coaE 3219574..3220191 (+) 618 WP_069047508.1 dephospho-CoA kinase -
  BSY239_RS15070 (BSY239_3053) zapD 3220234..3220989 (+) 756 WP_069047509.1 cell division protein ZapD -
  BSY239_RS15075 (BSY239_3054) - 3221009..3221212 (+) 204 WP_069047510.1 DNA gyrase inhibitor YacG -
  BSY239_RS15080 (BSY239_3055) - 3221223..3221684 (-) 462 WP_069047511.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62410.74 Da        Isoelectric Point: 5.6243

>NTDB_id=188361 BSY239_RS15050 WP_069047505.1 3215731..3217464(+) (pilB) [Hydrogenophaga sp. RAC07]
MASVESVTQESPSMALPGLGRALVSAGKLGQKAAEDLYRKAQSGRSSFIAELTGSGAVSAADLAHTMSAAFSAPLLDLDA
IDVQRLPSGLLDAKICADYRIVVLSKRNNRLMVATADPADQQAAEKIKFATQMGVDWVIAEYDKLSKLVDAQTTSVAETM
DNIIGGDFEFDEASTETVVTDANDKASEVDDAPVVKFLHKMLIDAFNMRASDLHFEPYEHTYRVRFRVDGELREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKVGIDALGYEPEEKER
LMAAIGRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQDPD
VIMVGEIRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCGVCKATLDVPR
KALVDAGFKADDLDGTWTPYRPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRGGSALDIAQQASKEGVRTLRESGLLK
VKLGLTSLEEVLSVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=188361 BSY239_RS15050 WP_069047505.1 3215731..3217464(+) (pilB) [Hydrogenophaga sp. RAC07]
ATGGCGTCCGTTGAATCTGTTACCCAGGAATCTCCGTCCATGGCCCTGCCGGGCCTGGGCCGTGCATTGGTGTCGGCTGG
CAAGCTCGGGCAAAAGGCCGCAGAAGACCTCTACCGCAAAGCCCAGAGTGGCCGCTCAAGCTTCATCGCTGAATTGACGG
GTTCAGGCGCCGTCTCCGCTGCAGACCTCGCGCACACCATGTCGGCGGCGTTCTCCGCCCCACTGCTGGACCTGGATGCG
ATCGATGTGCAGCGTTTGCCCAGTGGCCTGCTGGACGCAAAAATCTGCGCCGACTACCGCATCGTCGTTCTGAGCAAGCG
CAACAACCGCCTGATGGTGGCCACGGCGGACCCTGCCGATCAACAGGCGGCGGAAAAGATCAAGTTCGCCACGCAAATGG
GCGTGGATTGGGTGATTGCCGAATACGACAAGCTCAGCAAGCTGGTGGATGCGCAAACCACCAGCGTCGCGGAGACCATG
GACAACATCATCGGAGGCGACTTCGAATTCGATGAAGCGTCCACCGAAACCGTGGTCACGGACGCGAACGACAAGGCTTC
CGAGGTCGACGACGCGCCGGTGGTCAAGTTCCTTCACAAGATGCTGATCGACGCGTTCAACATGCGCGCCTCCGACCTGC
ATTTTGAGCCCTACGAACACACCTACCGTGTGCGTTTCCGGGTGGACGGCGAACTCCGCGAGATCGCCTCGCCCCCGATC
GCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCATGGACATCTCCGAGAAACGCGTGCCGCAAGACGG
TCGGATGAAGCTCAAGATCGGACCGGACCGGGTGATCGACTTCCGGGTGAGCACCCTGCCCACGCTCTTCGGCGAGAAGA
TCGTGATTCGTATCCTGGATCCCAGCAGTGCCAAGGTGGGCATCGATGCGCTGGGCTACGAACCGGAAGAGAAAGAGCGG
TTGATGGCCGCGATCGGCCGCCCCTATGGCATGGTGCTGGTGACCGGGCCCACGGGTTCAGGCAAGACCGTGTCGCTCTA
CACCTGCCTCAACATCCTGAACAAACCCGGGATCAACATCTCGACGGCAGAAGATCCCTCGGAGATCAATTTGCCCGGGG
TGAACCAGGTCAACGTGAACGAGAAGGCCGGCCTGACTTTCGCGGCTGCTCTGAAGGCTTTCCTGCGGCAGGATCCCGAC
GTGATCATGGTCGGTGAGATTCGCGACCTGGAGACAGCAGACATCTCCATCAAGGCCGCACAAACCGGCCACATGGTGTT
GTCGACGCTGCACACCAACGATGCACCGACCACGCTGACACGCATGATGAACATGGGCATTCCCACGTTCAACATTGCGT
CCAGTGTGATCCTGATCACTGCGCAGCGACTGGCTCGCCGCCTGTGCGGTGTTTGCAAGGCCACCCTGGATGTGCCCCGC
AAGGCTTTGGTGGATGCGGGTTTCAAGGCAGACGACCTTGACGGCACCTGGACGCCCTACCGTCCCGTGGGCTGCTCGGC
ATGCAACAACGGCTACAAGGGGCGCGTCGGTATTTACCAGGTGATGCCGATCTCCGAAGAAATTCAACGCATCATCCTGC
GCGGCGGCAGCGCGCTGGACATCGCCCAACAGGCCAGCAAGGAAGGCGTTCGCACATTGCGCGAATCCGGACTGCTCAAG
GTGAAGCTGGGCCTGACTTCACTGGAAGAAGTGCTCAGCGTCACCAACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.403

99.307

0.53
  pilB Acinetobacter baylyi ADP1

53.832

97.227

0.523
  pilF Neisseria gonorrhoeae MS11

53.004

98.094

0.52
  pilB Legionella pneumophila strain ERS1305867

50.177

98.094

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

44.306

97.4

0.432
  pilB Vibrio campbellii strain DS40M4

43.739

98.267

0.43
  pilB Vibrio cholerae strain A1552

47.683

89.775

0.428
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.258

98.614

0.367
  pilF Thermus thermophilus HB27

37.411

97.747

0.366

Multiple sequence alignment