Detailed information NTG0188363

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BSY239_RS15060
• Genome accession: NZ_CP016449
• Coordinates: 3218714..3219577 (+)
• Length: 287 a.a.
• NCBI ID: WP_069047507.1
• Uniprot ID: A0A1B3LW27
• Organism: Hydrogenophaga sp. RAC07
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3213714..3224577

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSY239_RS15035 (BSY239_3046)	rplU	3213925..3214236 (-)	312	WP_069047504.1	50S ribosomal protein L21	-
BSY239_RS15040 (BSY239_3047)	-	3214504..3215433 (+)	930	WP_083240201.1	polyprenyl synthetase family protein	-
BSY239_RS15050 (BSY239_3049)	pilB	3215731..3217464 (+)	1734	WP_069047505.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BSY239_RS15055 (BSY239_3050)	pilC	3217497..3218714 (+)	1218	WP_069047506.1	type II secretion system F family protein	Machinery gene
BSY239_RS15060 (BSY239_3051)	pilD	3218714..3219577 (+)	864	WP_069047507.1	A24 family peptidase	Machinery gene
BSY239_RS15065 (BSY239_3052)	coaE	3219574..3220191 (+)	618	WP_069047508.1	dephospho-CoA kinase	-
BSY239_RS15070 (BSY239_3053)	zapD	3220234..3220989 (+)	756	WP_069047509.1	cell division protein ZapD	-
BSY239_RS15075 (BSY239_3054)	-	3221009..3221212 (+)	204	WP_069047510.1	DNA gyrase inhibitor YacG	-
BSY239_RS15080 (BSY239_3055)	-	3221223..3221684 (-)	462	WP_069047511.1	NUDIX domain-containing protein	-
BSY239_RS15085 (BSY239_3056)	-	3221681..3222559 (-)	879	WP_069047512.1	ATP-binding protein	-
BSY239_RS15090 (BSY239_3057)	argJ	3222570..3223817 (-)	1248	WP_069047513.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 30585.03 Da
• Isoelectric Point: 5.9776

>NTDB_id=188363 BSY239_RS15060 WP_069047507.1 3218714..3219577(+) (pilD) [Hydrogenophaga sp. RAC07]
MLGAGAPEAALLGLLGLLVGSFLNVVIHRLPKMMELHWAAECAELHPENATASDTAAATAQPFNLMVPRSRCPHCGHQIR
WFENIPLFSYLALRGKCSQCAAPISARYPAVEVTCAALFAWCGWNWGGSWEALAWCGFSAAVLALACIDWDTTLLPDDIT
LPLLWAGLCAAGLKLTDTALPDAVWGAVGGYLSLWLVYWAFKLTTGKEGMGYGDFKLFAALGAWFGWQALIPIILMASVI
GAVIGIAIKFRGELREGGYVPFGPFLALAGLTAMIFGPSAILAVIGL

Nucleotide

• Length: 864 bp

>NTDB_id=188363 BSY239_RS15060 WP_069047507.1 3218714..3219577(+) (pilD) [Hydrogenophaga sp. RAC07]
ATGCTCGGTGCGGGTGCGCCGGAAGCGGCGCTGCTGGGTCTTCTCGGCTTGCTGGTGGGCAGCTTTCTCAATGTGGTGAT
CCACCGCCTGCCCAAGATGATGGAGTTGCACTGGGCCGCCGAGTGCGCGGAACTCCATCCTGAAAACGCCACGGCTTCCG
ACACGGCGGCTGCCACAGCCCAGCCGTTCAACCTCATGGTGCCGCGCTCGCGCTGCCCCCACTGTGGCCACCAGATTCGC
TGGTTCGAGAACATCCCGCTCTTCAGCTACCTCGCACTGCGCGGCAAATGCAGCCAATGCGCGGCACCGATCAGTGCCCG
CTACCCGGCGGTGGAGGTGACCTGCGCAGCACTGTTTGCCTGGTGCGGCTGGAACTGGGGCGGCAGTTGGGAAGCGCTTG
CCTGGTGCGGATTTTCCGCGGCCGTGTTGGCGTTGGCCTGCATCGATTGGGACACCACCTTGTTGCCGGACGACATCACG
CTTCCCTTGTTGTGGGCAGGCCTGTGCGCGGCGGGTCTGAAGCTGACCGATACCGCTCTGCCCGATGCGGTGTGGGGCGC
CGTGGGAGGCTACCTCTCGTTGTGGCTGGTGTACTGGGCCTTCAAGCTCACCACCGGCAAGGAAGGCATGGGCTACGGCG
ACTTCAAACTCTTCGCCGCCCTGGGCGCCTGGTTTGGCTGGCAGGCCCTCATCCCCATCATCCTCATGGCCTCGGTCATC
GGGGCGGTGATCGGCATCGCCATCAAATTCAGAGGTGAGCTGCGCGAAGGCGGGTACGTGCCGTTCGGCCCCTTCCTGGC
GCTCGCCGGCCTCACCGCCATGATCTTCGGGCCGTCGGCCATCCTGGCCGTGATTGGCTTGTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-125)

3D structure

• AlphaFold DB: A0A1B3LW27

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.045	93.728	0.488
pilD	Vibrio campbellii strain DS40M4	50.566	92.334	0.467
pilD	Neisseria gonorrhoeae MS11	47.308	90.592	0.429
pilD	Acinetobacter baumannii D1279779	43.321	96.516	0.418
pilD	Acinetobacter nosocomialis M2	42.96	96.516	0.415