Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   clem_RS04465 Genome accession   NZ_CP016397
Coordinates   1027615..1029081 (+) Length   488 a.a.
NCBI ID   WP_232505562.1    Uniprot ID   A0A222P0U6
Organism   Legionella clemsonensis strain CDC-D5610     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022615..1034081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  clem_RS04440 (clem_04520) - 1023738..1024664 (-) 927 WP_094090514.1 hypothetical protein -
  clem_RS04445 (clem_04525) pilM 1024921..1025991 (+) 1071 WP_157698178.1 type IV pilus biogenesis protein PilM -
  clem_RS04450 (clem_04530) - 1025978..1026529 (+) 552 WP_094090516.1 PilN domain-containing protein -
  clem_RS04455 (clem_04535) - 1026519..1027118 (+) 600 WP_094090517.1 type 4a pilus biogenesis protein PilO -
  clem_RS04460 (clem_04540) - 1027115..1027612 (+) 498 WP_094090518.1 pilus assembly protein PilP -
  clem_RS04465 (clem_04545) pilQ 1027615..1029081 (+) 1467 WP_232505562.1 type IV pilus secretin PilQ Machinery gene
  clem_RS04470 (clem_04550) aroK 1029161..1029688 (+) 528 WP_045106431.1 shikimate kinase AroK -
  clem_RS04475 (clem_04555) aroB 1029675..1030778 (+) 1104 WP_094090520.1 3-dehydroquinate synthase -
  clem_RS04480 (clem_04560) - 1030782..1032293 (+) 1512 WP_094090521.1 AAA family ATPase -
  clem_RS04485 (clem_04565) pnuC 1032277..1032867 (-) 591 WP_094090522.1 nicotinamide riboside transporter PnuC -
  clem_RS04490 (clem_04570) - 1033080..1033508 (+) 429 WP_094090523.1 universal stress protein -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 54776.79 Da        Isoelectric Point: 10.3732

>NTDB_id=187966 clem_RS04465 WP_232505562.1 1027615..1029081(+) (pilQ) [Legionella clemsonensis strain CDC-D5610]
MHKILLGLCLLLFNINVIAQNSLQHPKIKKEVENITKARIIKNKVGNKRISLNFQDIEVRAVLQLLAEFTGINMVISDTV
KGNITLSLNDIAWEQALSIILTTQSLEKRRMGNIMFIAPAEEFTKREKKIRQAQDETNKFTPLQSLLFRIKYAKAADIAN
VLQEKNEAFLSRRGRIAVDIRTNSLWVQDSYKHLKIIRKLIEQLDIPVKQVLIEARIVNVTKDFAQDLGLRFGVARPTHL
GALKKHDSLIKSSDSAEGVSLKERLNFDLDSLPMATSPVSVGIALAKLSNHILLDLELSALESEGRGEVISTPRLIATNQ
QPALIESGEEIPYQEATSSGATAVVFKKAVLSLKVTPQITPDSNILMDLKINQDIPSPKVFNGVPTILTKEIQTSVLVEN
GRTIVLGGIYKQDKNNEINRTPLLGDLPVVRHLFRNRSLSTRNEELLIFITPRIISNNLSSKSFDSSKQYMMRGVKLTKF
GRQLEKYH

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=187966 clem_RS04465 WP_232505562.1 1027615..1029081(+) (pilQ) [Legionella clemsonensis strain CDC-D5610]
TTGCATAAAATACTTCTGGGATTGTGCTTATTACTTTTTAACATAAATGTTATAGCCCAAAATAGTTTACAGCACCCTAA
AATAAAGAAAGAAGTAGAAAATATAACGAAAGCTAGGATCATTAAAAATAAAGTAGGTAATAAACGAATTTCTTTAAATT
TTCAAGATATTGAGGTAAGAGCTGTTTTGCAGTTATTAGCTGAATTTACTGGAATAAATATGGTTATAAGTGATACAGTA
AAGGGTAATATCACCTTGAGTTTAAATGATATAGCCTGGGAACAGGCTCTGAGTATTATTTTAACAACCCAATCTCTAGA
GAAAAGACGCATGGGCAATATCATGTTTATCGCACCTGCCGAAGAGTTCACTAAACGTGAGAAAAAAATTCGACAGGCAC
AAGATGAGACGAATAAATTTACGCCATTACAGTCATTGTTATTTCGAATTAAGTATGCCAAAGCTGCCGATATTGCTAAT
GTTTTGCAAGAAAAAAATGAAGCATTTTTATCAAGACGTGGCAGAATTGCTGTTGATATAAGAACAAACAGCTTATGGGT
GCAAGACAGTTATAAGCACTTAAAAATTATTAGAAAACTCATTGAGCAACTCGATATTCCAGTAAAACAAGTTTTAATTG
AAGCGCGTATTGTTAATGTTACTAAAGATTTTGCTCAAGATTTAGGATTGCGATTTGGAGTTGCAAGACCAACTCATTTG
GGTGCTCTAAAGAAACACGACTCTCTTATAAAATCATCAGACTCGGCTGAAGGTGTTTCATTGAAGGAGAGATTAAATTT
TGATCTTGACAGCTTGCCTATGGCAACCTCACCTGTGTCTGTAGGAATAGCCTTAGCCAAGCTCAGCAATCATATCCTTT
TGGATTTGGAGTTATCGGCACTTGAAAGCGAGGGGCGGGGCGAAGTAATATCCACTCCAAGATTGATAGCAACCAATCAA
CAGCCTGCGTTAATCGAATCCGGAGAAGAAATTCCCTATCAGGAGGCGACTTCAAGCGGGGCAACTGCTGTAGTGTTTAA
AAAAGCTGTGTTAAGTTTGAAAGTAACACCGCAAATTACTCCGGATAGCAATATTTTGATGGATTTAAAAATTAATCAGG
ATATACCATCACCAAAAGTTTTTAATGGTGTACCTACAATTCTGACAAAAGAAATTCAAACGAGTGTTTTAGTAGAGAAT
GGTCGTACAATTGTCTTGGGAGGAATTTATAAACAAGATAAAAATAATGAGATTAATCGCACGCCTCTCCTAGGTGATTT
ACCGGTTGTGAGACATTTATTCAGGAATCGCTCTCTATCAACCAGAAATGAGGAGTTGCTCATTTTCATTACTCCTAGGA
TAATATCCAACAATTTATCGTCAAAATCCTTTGACAGTTCAAAGCAATACATGATGAGAGGTGTAAAATTAACAAAGTTT
GGTAGACAATTAGAAAAATATCATTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(300-455)

Bacterial type II/III secretion system short domain (Secretin_N)

(147-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222P0U6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

64.651

88.115

0.57
  pilQ Legionella pneumophila strain Lp02

64.651

88.115

0.57
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

47.356

85.246

0.404
  pilQ Vibrio cholerae strain A1552

47.356

85.246

0.404
  pilQ Acinetobacter baumannii strain A118

39.796

100

0.4
  pilQ Acinetobacter baumannii D1279779

39.796

100

0.4
  comQ Acinetobacter baylyi ADP1

43.694

90.984

0.398
  pilQ Vibrio campbellii strain DS40M4

45.412

87.09

0.395
  pilQ Pseudomonas aeruginosa PAK

41.452

87.5

0.363

Multiple sequence alignment