Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TK34_RS02400 Genome accession   NZ_CP016380
Coordinates   513106..514812 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AHNIH1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508106..519812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK34_RS02370 (TK34_02345) - 508185..509147 (-) 963 WP_065475368.1 IS1595 family transposase -
  TK34_RS02375 (TK34_02350) yacG 509256..509450 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  TK34_RS02380 (TK34_02355) zapD 509460..510182 (-) 723 WP_065475370.1 cell division protein ZapD -
  TK34_RS02385 (TK34_02360) coaE 510220..510834 (-) 615 WP_065475372.1 dephospho-CoA kinase -
  TK34_RS02390 (TK34_02365) pilD 510854..511726 (-) 873 WP_065475375.1 prepilin peptidase Machinery gene
  TK34_RS02395 (TK34_02370) pilC 511749..512990 (-) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  TK34_RS02400 (TK34_02375) pilB 513106..514812 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  TK34_RS22920 (TK34_02380) tapA 514816..515244 (-) 429 WP_081304776.1 type IVa pilus major pilin TapA -
  TK34_RS02410 (TK34_02390) nadC 515567..516424 (-) 858 WP_065475376.1 carboxylating nicotinate-nucleotide diphosphorylase -
  TK34_RS02415 (TK34_02395) - 516430..516900 (-) 471 WP_024944772.1 retropepsin-like aspartic protease family protein -
  TK34_RS02420 (TK34_02400) ampD 517048..517620 (+) 573 WP_065475378.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  TK34_RS02425 (TK34_02405) pdhR 517896..518660 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=187629 TK34_RS02400 WP_029302067.1 513106..514812(-) (pilB) [Aeromonas hydrophila strain AHNIH1]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=187629 TK34_RS02400 WP_029302067.1 513106..514812(-) (pilB) [Aeromonas hydrophila strain AHNIH1]
ATGACTTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCTCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAATGAAATCTTGGACAGCAAGGCATTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTACCCATCTATACCCAAGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTAGAAGAGAACA
AACTCACTACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCGCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTCCACTTCGAACCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGGATCCTGCATGAAATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTCTCGGCTCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCACAGGATGGCCGCATAAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTTTGCTCGACTCCTCCG
CCGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATATCTGCGAGCCCTGTCGAAGCCTCAG
GGCATGATTCTGGTAACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGTGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCAGAAATTGCCATCAAGGCCGCTCAGACCGGCCACCTGGTGCTCTCCACTCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAATATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCAGGTTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAAAGGCCGAGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTAGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment