Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BA894_RS12685 Genome accession   NZ_CP016351
Coordinates   2830060..2831283 (+) Length   407 a.a.
NCBI ID   WP_065297364.1    Uniprot ID   -
Organism   Vibrio natriegens strain CCUG 16374     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825060..2836283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA894_RS12665 (BA894_12665) ampD 2825831..2826376 (-) 546 WP_065297360.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BA894_RS12670 (BA894_12670) nadC 2826776..2827663 (+) 888 WP_065297361.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BA894_RS12675 (BA894_12675) - 2827922..2828341 (+) 420 WP_065297362.1 type IV pilin protein -
  BA894_RS12680 (BA894_12680) pilB 2828344..2830029 (+) 1686 WP_065297363.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BA894_RS12685 (BA894_12685) pilC 2830060..2831283 (+) 1224 WP_065297364.1 type II secretion system F family protein Machinery gene
  BA894_RS12690 (BA894_12690) pilD 2831351..2832220 (+) 870 WP_065297365.1 prepilin peptidase Machinery gene
  BA894_RS12695 (BA894_12695) coaE 2832221..2832835 (+) 615 WP_014232954.1 dephospho-CoA kinase -
  BA894_RS12700 (BA894_12700) zapD 2832862..2833602 (+) 741 WP_014232955.1 cell division protein ZapD -
  BA894_RS12705 (BA894_12705) yacG 2833676..2833870 (+) 195 WP_065297366.1 DNA gyrase inhibitor YacG -
  BA894_RS12710 (BA894_12710) rplS 2834233..2834586 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  BA894_RS12715 (BA894_12715) trmD 2834627..2835388 (-) 762 WP_014232957.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  BA894_RS12720 (BA894_12720) rimM 2835416..2835964 (-) 549 WP_014232958.1 ribosome maturation factor RimM -
  BA894_RS12725 (BA894_12725) rpsP 2835995..2836243 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45098.31 Da        Isoelectric Point: 10.3231

>NTDB_id=187259 BA894_RS12685 WP_065297364.1 2830060..2831283(+) (pilC) [Vibrio natriegens strain CCUG 16374]
MKTTTPQLKNFRWKGINSSGKKTSGQTLAMSEVEVRERLSAQHIKIKKLKKSSISFLTKMSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSNNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASAHFDALYTDLISTGELSGNLAEVFERLANYREK
SEELRAKVIKALIYPAMVMLVALGVSYLMLTQVIPEFEKMFSGFGADLPWFTRQVLDLSAWMQNWGSFIALGTLSLVISA
RILVKRSDSFRLMMDRAILRIPVLGAVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTSGNLHYQLAVEEVYRDTAAG
MPMYVAMRNCDVFPELVLQMVMIGEESGRLDEMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=187259 BA894_RS12685 WP_065297364.1 2830060..2831283(+) (pilC) [Vibrio natriegens strain CCUG 16374]
ATGAAAACGACGACCCCACAACTAAAGAACTTCCGCTGGAAAGGCATTAATAGCTCAGGAAAAAAAACTTCTGGGCAAAC
GCTGGCCATGAGCGAAGTGGAAGTACGAGAACGACTCAGCGCTCAGCACATAAAAATAAAAAAACTGAAAAAAAGCAGTA
TTTCTTTTTTAACCAAAATGAGCCATCGGGTAAAAGGCAAAGACATCACCGTTTTTACTCGCCAAATTTCGACTATGCTG
GTCACCGGTGTCCCACTCGTTCAGGCGTTGAAATTGGTTTCCAACAACCATAAAAAAGCCGAGATGAAATCTATTTTAAT
GAGCGTAACTCGAGCAGTTGAAGCGGGTACGCCGATGTCAAAAGCAATGCGCACGGCCAGCGCACATTTTGACGCACTTT
ATACTGACCTTATCTCAACCGGAGAACTCTCAGGCAATCTGGCAGAAGTCTTCGAACGACTGGCCAATTACCGTGAAAAA
AGTGAGGAACTCAGAGCGAAAGTCATCAAGGCCCTGATCTACCCAGCCATGGTTATGTTGGTTGCGCTCGGCGTGTCCTA
TCTCATGCTCACTCAAGTTATCCCTGAGTTTGAAAAAATGTTTTCTGGGTTTGGTGCAGATTTACCTTGGTTTACCAGAC
AAGTACTAGACTTGTCTGCGTGGATGCAAAATTGGGGTTCATTCATCGCTCTTGGGACACTTAGCCTAGTTATATCAGCA
AGAATACTGGTCAAACGTTCTGATTCTTTTCGCTTGATGATGGATCGAGCCATATTGAGAATTCCCGTTCTTGGCGCTGT
GTTGTCCAAGGCGGCGATTGCTAAATTTAGCCGAACCCTGGCCACCAGCTTCAGTGCTGGTATTCCTATTCTGACCAGTT
TAAAAACCACCTCAAAAACATCAGGAAACCTGCATTATCAGCTGGCAGTAGAAGAGGTCTATCGTGATACAGCCGCTGGT
ATGCCAATGTATGTCGCGATGCGTAACTGCGATGTTTTCCCTGAATTAGTGCTACAAATGGTGATGATTGGAGAAGAATC
AGGCAGGCTTGATGAGATGCTCAATAAAGTCGCAACTATTTATGAGTTTGAAGTAGATAATACCGTCGATAACTTAAGTA
AGATTCTTGAACCACTGATTATCGTTTTTCTTGGTGTGGTCGTAGGTGGATTAGTGACAGCAATGTACCTGCCAATCTTT
AACTTAATGAGTGTATTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.258

100

0.853
  pilC Vibrio cholerae strain A1552

72.84

99.509

0.725
  pilC Acinetobacter baylyi ADP1

42.786

98.771

0.423
  pilC Legionella pneumophila strain ERS1305867

40.988

99.509

0.408
  pilC Pseudomonas stutzeri DSM 10701

40.15

98.526

0.396
  pilC Acinetobacter baumannii D1279779

40.25

98.28

0.396
  pilG Neisseria gonorrhoeae MS11

39.702

99.017

0.393
  pilG Neisseria meningitidis 44/76-A

39.454

99.017

0.391

Multiple sequence alignment