Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EC725_RS22825 Genome accession   NZ_CP020092
Coordinates   4297987..4299360 (+) Length   457 a.a.
NCBI ID   WP_032314426.1    Uniprot ID   -
Organism   Escherichia coli strain 13E0725     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4292987..4304360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC725_RS22795 (EC725_22800) aroP 4293535..4294908 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  EC725_RS22800 (EC725_22805) ampE 4294951..4295805 (-) 855 WP_000172007.1 beta-lactamase regulator AmpE -
  EC725_RS22805 (EC725_22810) ampD 4295802..4296353 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EC725_RS22810 (EC725_22815) nadC 4296441..4297334 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EC725_RS22820 (EC725_22825) pilA 4297537..4297977 (+) 441 WP_000360906.1 prepilin peptidase-dependent pilin Machinery gene
  EC725_RS22825 (EC725_22830) pilB 4297987..4299360 (+) 1374 WP_032314426.1 type II secretion system protein GspE Machinery gene
  EC725_RS22830 (EC725_22835) hofC 4299350..4300552 (+) 1203 WP_032314427.1 protein transport protein HofC -
  EC725_RS22835 (EC725_22840) guaC 4300587..4301630 (-) 1044 WP_001217338.1 GMP reductase -
  EC725_RS27600 - 4301786..4301830 (-) 45 WP_120795372.1 protein YacM -
  EC725_RS22840 (EC725_22845) coaE 4301855..4302475 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  EC725_RS22845 (EC725_22850) zapD 4302475..4303218 (+) 744 WP_001194734.1 cell division protein ZapD -
  EC725_RS22850 (EC725_22855) yacG 4303228..4303425 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  EC725_RS22855 (EC725_22860) mutT 4303524..4303913 (-) 390 WP_000736004.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50221.93 Da        Isoelectric Point: 6.7135

>NTDB_id=184765 EC725_RS22825 WP_032314426.1 4297987..4299360(+) (pilB) [Escherichia coli strain 13E0725]
MNIPQLTALCLRYQGVLLDASEEVVHIAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVELAGNA
VSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLNTADI
NICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCET
LVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFEVLPI
TPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=184765 EC725_RS22825 WP_032314426.1 4297987..4299360(+) (pilB) [Escherichia coli strain 13E0725]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATAT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGCCGGAGTCGCATTAACCGCCAGATTAA
AAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTGGCAGGAAACGCC
GTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCAGGTGGGTCAGGC
ACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAACCACAGGGACTGG
TGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAATACCGCTGACATT
AATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCCGCGTGCCGGACT
CACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCCGCGATGGCGAAA
CAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCCACCTGCGAAACG
CTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGCCCAGCGTCTGGT
ACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGCCATCGCCGCTGC
CCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAAGTTCTGCCCATA
ACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACAGGCGGGTATGCG
TACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCGTACTGGGGATGC
CGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.262

83.589

0.42
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.418
  pilB Acinetobacter baylyi ADP1

40.171

100

0.411
  pilB Vibrio campbellii strain DS40M4

48.446

84.464

0.409
  pilB Vibrio cholerae strain A1552

46.923

85.339

0.4
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

85.339

0.396
  pilB Haemophilus influenzae 86-028NP

44.961

84.683

0.381
  pilB Acinetobacter baumannii D1279779

43.655

86.214

0.376
  pilB Haemophilus influenzae Rd KW20

44.186

84.683

0.374
  pilF Neisseria gonorrhoeae MS11

44.125

83.807

0.37
  pilF Thermus thermophilus HB27

40.587

89.497

0.363