Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EC725_RS22820 Genome accession   NZ_CP020092
Coordinates   4297537..4297977 (+) Length   146 a.a.
NCBI ID   WP_000360906.1    Uniprot ID   -
Organism   Escherichia coli strain 13E0725     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4292537..4302977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC725_RS22795 (EC725_22800) aroP 4293535..4294908 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  EC725_RS22800 (EC725_22805) ampE 4294951..4295805 (-) 855 WP_000172007.1 beta-lactamase regulator AmpE -
  EC725_RS22805 (EC725_22810) ampD 4295802..4296353 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EC725_RS22810 (EC725_22815) nadC 4296441..4297334 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EC725_RS22820 (EC725_22825) pilA 4297537..4297977 (+) 441 WP_000360906.1 prepilin peptidase-dependent pilin Machinery gene
  EC725_RS22825 (EC725_22830) pilB 4297987..4299360 (+) 1374 WP_032314426.1 type II secretion system protein GspE Machinery gene
  EC725_RS22830 (EC725_22835) hofC 4299350..4300552 (+) 1203 WP_032314427.1 protein transport protein HofC -
  EC725_RS22835 (EC725_22840) guaC 4300587..4301630 (-) 1044 WP_001217338.1 GMP reductase -
  EC725_RS27600 - 4301786..4301830 (-) 45 WP_120795372.1 protein YacM -
  EC725_RS22840 (EC725_22845) coaE 4301855..4302475 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15651.78 Da        Isoelectric Point: 4.3938

>NTDB_id=184764 EC725_RS22820 WP_000360906.1 4297537..4297977(+) (pilA) [Escherichia coli strain 13E0725]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVTKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=184764 EC725_RS22820 WP_000360906.1 4297537..4297977(+) (pilA) [Escherichia coli strain 13E0725]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGACAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363