Detailed information NTG0183228

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC063_RS08535
• Genome accession: NZ_CP015905
• Coordinates: 1739622..1740269 (-)
• Length: 215 a.a.
• NCBI ID: WP_058225668.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain UC063
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1734622..1745269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC063_RS08505 (LLUC063_1670)	atpF	1734683..1735189 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
LLUC063_RS08510 (LLUC063_1671)	atpB	1735204..1735917 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLUC063_RS08515 (LLUC063_1672)	-	1735962..1736177 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLUC063_RS08520 (LLUC063_1673)	-	1736361..1737137 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLUC063_RS08525 (LLUC063_1674)	comEC	1737422..1738981 (-)	1560	WP_345893333.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLUC063_RS08530 (LLUC063_1675)	comEC	1738999..1739532 (-)	534	WP_235589824.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC063_RS08535 (LLUC063_1676)	comEA	1739622..1740269 (-)	648	WP_058225668.1	ComEA family DNA-binding protein	Machinery gene
LLUC063_RS08540 (LLUC063_1677)	-	1740329..1741690 (-)	1362	WP_021214597.1	ABC transporter permease	-
LLUC063_RS08545 (LLUC063_1678)	-	1741687..1742619 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLUC063_RS08550 (LLUC063_1679)	-	1742724..1743122 (-)	399	WP_004255236.1	hypothetical protein	-
LLUC063_RS08555 (LLUC063_1680)	-	1743226..1743789 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLUC063_RS08560 (LLUC063_1681)	-	1743962..1745140 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23650.89 Da
• Isoelectric Point: 5.0062

>NTDB_id=183228 LLUC063_RS08535 WP_058225668.1 1739622..1740269(-) (comEA) [Lactococcus lactis subsp. lactis strain UC063]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKIGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=183228 LLUC063_RS08535 WP_058225668.1 1739622..1740269(-) (comEA) [Lactococcus lactis subsp. lactis strain UC063]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAATTGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-212)

• SLBB domain (SLBB) (70-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367