Detailed information NTG0182499

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: A8L59_RS13865
• Genome accession: NZ_CP015852
• Coordinates: 3187984..3189096 (-)
• Length: 370 a.a.
• NCBI ID: WP_064587720.1
• Uniprot ID: A0AAC9BV99
• Organism: Pseudomonas koreensis strain CRS05-R5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3182984..3194096

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A8L59_RS13850 (A8L59_13840)	-	3183627..3185018 (+)	1392	WP_064587717.1	GntP family permease	-
A8L59_RS13855 (A8L59_13845)	hbdH	3185026..3185799 (+)	774	WP_064587718.1	3-hydroxybutyrate dehydrogenase	-
A8L59_RS13860 (A8L59_13850)	-	3185981..3187936 (+)	1956	WP_064587719.1	acetoacetate--CoA ligase	-
A8L59_RS13865 (A8L59_13855)	pilU	3187984..3189096 (-)	1113	WP_064587720.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
A8L59_RS13870 (A8L59_13860)	-	3189230..3191779 (+)	2550	WP_064587721.1	PAS domain S-box protein	-
A8L59_RS13875 (A8L59_13865)	-	3191819..3192094 (+)	276	WP_003225447.1	peptidylprolyl isomerase	-
A8L59_RS13880 (A8L59_13870)	-	3192206..3193141 (-)	936	WP_064587722.1	sugar kinase	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41249.22 Da
• Isoelectric Point: 6.8784

>NTDB_id=182499 A8L59_RS13865 WP_064587720.1 3187984..3189096(-) (pilU) [Pseudomonas koreensis strain CRS05-R5]
MEIDALLIRLSSQHGSDLFLSTGAPPSARIDGVLTPFSERPFKPGEVQAIAHSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKRSIINQREVGVDTRSFQAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVLSTLHAHNANQALDRI
VNLFPEERRPQLLHALGNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIADLIRRNELGELKAIMDKSAEVGMQTFDAAL
YALVVEGVISEDEALKHADSQNNLRLRLKLHAEAAPTPQPPSSGDWGLMD

Nucleotide

• Length: 1113 bp

>NTDB_id=182499 A8L59_RS13865 WP_064587720.1 3187984..3189096(-) (pilU) [Pseudomonas koreensis strain CRS05-R5]
ATGGAAATCGATGCACTGTTGATCCGCCTGTCGAGCCAGCACGGCTCCGACCTGTTTCTGTCCACGGGCGCGCCACCGAG
TGCGCGCATCGACGGGGTGCTGACGCCGTTCAGCGAGCGACCGTTCAAGCCCGGTGAAGTCCAGGCCATCGCCCACTCCC
TGATGGACGCCGAGCAGCGGCGCGAGTTCGATCGGGATCTGGAAATGAACCTGGCGATTTCACGCACCGGCATCGGGCGC
TTTCGGGTGAACATCTTCAAGCAACGTAACGACGTGTCGATTGTGATCCGCAACGTCAAACTCGACATCCCGCGCTTCGA
AGACCTCAAACTGCCGCCGGTGCTGCTGGAAACGGTGATGCTCAAACAGGGGTTGATCCTGTTCGTCGGCGCCACCGATT
CCGGCAAATCCACCTCGCTGGCCGCGCTGATCGATCACCGCAACCGGCACAGCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGCCACAAGCGTTCGATCATCAATCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTCCAGGC
CGCGCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTGTTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCATG
CCCTGACGTTCGCCGACACCGGGCACCTGGTGCTGTCGACCCTGCACGCGCACAACGCCAATCAGGCGCTGGACCGAATC
GTCAATCTGTTCCCCGAAGAGCGCCGCCCGCAGCTGCTGCATGCGCTGGGCAACAATCTCAAGGCGTTTGTCTCGCAACG
GCTGGTGCGCACGCTGGATGGTCAGCGGCGGGCGGCGGTCGAAGTGATGCTGGGCACACCGACCATCGCCGACCTGATCC
GCCGCAATGAGTTGGGCGAACTCAAGGCGATCATGGACAAGTCTGCCGAGGTCGGCATGCAGACCTTCGACGCGGCGCTG
TACGCGCTGGTGGTGGAAGGGGTGATCAGCGAAGACGAGGCGCTCAAGCATGCCGATTCACAAAACAATCTGCGCCTGCG
TCTGAAGCTGCATGCCGAAGCGGCGCCAACGCCTCAACCTCCATCATCGGGCGACTGGGGCTTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (68-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.946	0.541
pilU	Acinetobacter baylyi ADP1	53.09	96.216	0.511
pilU	Vibrio cholerae strain A1552	54	94.595	0.511
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.018	90.27	0.37
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	39.595	93.514	0.37
pilT	Vibrio cholerae strain A1552	39.595	93.514	0.37
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.973	0.368
pilT	Pseudomonas aeruginosa PAK	39.244	92.973	0.365