Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A7318_RS03335 Genome accession   NZ_CP015639
Coordinates   771287..772495 (-) Length   402 a.a.
NCBI ID   WP_069021709.1    Uniprot ID   -
Organism   Pseudomonas lurida strain L228     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766287..777495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7318_RS03295 (A7318_03295) - 767205..767378 (-) 174 WP_016976295.1 DUF3094 family protein -
  A7318_RS03300 (A7318_03300) - 767433..768062 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  A7318_RS03305 (A7318_03305) - 768166..768633 (+) 468 WP_034102088.1 hypothetical protein -
  A7318_RS03310 (A7318_03310) - 768630..769319 (+) 690 WP_069021700.1 energy-coupling factor ABC transporter permease -
  A7318_RS03315 (A7318_03315) - 769395..769610 (+) 216 WP_069021702.1 hypothetical protein -
  A7318_RS03320 (A7318_03320) yacG 769607..769807 (-) 201 WP_034102092.1 DNA gyrase inhibitor YacG -
  A7318_RS03325 (A7318_03325) coaE 769804..770427 (-) 624 WP_069021704.1 dephospho-CoA kinase -
  A7318_RS03330 (A7318_03330) pilD 770424..771290 (-) 867 WP_069021707.1 prepilin peptidase Machinery gene
  A7318_RS03335 (A7318_03335) pilC 771287..772495 (-) 1209 WP_069021709.1 type II secretion system F family protein Machinery gene
  A7318_RS03340 (A7318_03340) - 772698..773111 (+) 414 WP_069078682.1 pilin -
  A7318_RS03345 (A7318_03345) - 773147..774547 (+) 1401 WP_069021711.1 O-antigen ligase family protein -
  A7318_RS03355 (A7318_03355) - 774828..775133 (+) 306 WP_034102101.1 DUF6388 family protein -
  A7318_RS03360 (A7318_03360) nadC 775208..776056 (-) 849 WP_069021713.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43314.77 Da        Isoelectric Point: 10.2543

>NTDB_id=181611 A7318_RS03335 WP_069021709.1 771287..772495(-) (pilC) [Pseudomonas lurida strain L228]
MDNASTLYAWEGINRKGRRVSGQTTGHSVARVKVQLRQQGISPGHVRKKSPMLPSFAPSIKPADITLFTRQLATLLKAGI
PLLQAFDVIGEGFDNRPMRKLVQGLKQAIAAGTSLAEALRKQPHYFDALYCNLVAAGEQAGALETLLERVAIHREKSEQL
KARIKKAMTYPITVLVVASLVTGVLLVHVVPQFQTLFAGVDGKLPGFTLQVIALSEFMQKAWWIIALGILAAGLGLRHAY
RTFADVRAWLDSCLLKTPLAGKLLSKSAVARYARTLSTTFAAGVPLVQALDSVAGAAGSGPFRQAIEHMRRDVATGMQLN
QSMVASGLFPRMAIQMTAIGEESGTLDSMLEKVASHYEADVDNVVDNLTNLMEPLIMVVLGGIVGALVVAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=181611 A7318_RS03335 WP_069021709.1 771287..772495(-) (pilC) [Pseudomonas lurida strain L228]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCAGACCACCGGGCA
CAGCGTTGCAAGGGTCAAGGTTCAGTTACGCCAACAGGGCATCAGCCCTGGGCATGTGCGCAAGAAATCCCCAATGCTGC
CGAGCTTCGCACCCTCGATCAAGCCGGCGGACATCACCCTGTTCACGCGCCAACTGGCTACGCTGCTCAAGGCCGGGATT
CCTCTCCTGCAAGCCTTCGACGTCATCGGTGAGGGTTTCGACAACCGGCCCATGCGTAAGCTGGTGCAAGGGTTGAAACA
GGCGATTGCCGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAGCAACCTCATTATTTCGACGCGCTGTACTGCAACCTGG
TAGCCGCCGGCGAACAGGCCGGAGCCCTTGAAACCTTGCTGGAACGCGTGGCAATCCACCGAGAGAAGAGCGAACAGCTA
AAGGCCAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGTCTCGTCACCGGCGTGCTGCTGGT
CCACGTCGTGCCACAGTTCCAGACGCTGTTCGCCGGCGTCGACGGCAAGCTGCCTGGCTTCACATTGCAGGTGATCGCCC
TTTCCGAGTTCATGCAGAAAGCCTGGTGGATCATCGCGCTGGGCATTCTTGCGGCAGGCTTGGGGTTGCGCCACGCCTAC
CGTACCTTCGCCGATGTTCGTGCCTGGCTGGACAGCTGTTTGTTGAAAACGCCCCTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTACGCCCGCACGCTTTCCACCACGTTTGCCGCCGGCGTTCCGTTGGTGCAGGCGCTGGACTCGGTGG
CCGGGGCCGCCGGTTCAGGGCCGTTCAGACAGGCAATCGAACATATGCGACGCGACGTAGCGACGGGCATGCAATTGAAT
CAATCCATGGTCGCCAGCGGCCTGTTTCCCCGCATGGCAATCCAGATGACAGCCATCGGCGAAGAATCAGGCACGCTGGA
CAGCATGCTGGAAAAGGTCGCCAGCCATTACGAGGCCGATGTGGATAACGTGGTCGATAACCTCACCAACCTTATGGAGC
CGCTGATTATGGTGGTACTGGGAGGGATCGTCGGCGCACTGGTGGTCGCGATGTATTTACCGGTATTCCAGTTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.596

98.507

0.587
  pilC Acinetobacter baumannii D1279779

51.646

98.259

0.507
  pilC Acinetobacter baylyi ADP1

51.263

98.507

0.505
  pilC Legionella pneumophila strain ERS1305867

48.861

98.259

0.48
  pilC Vibrio cholerae strain A1552

41.919

98.507

0.413
  pilG Neisseria gonorrhoeae MS11

40.992

95.274

0.391
  pilC Vibrio campbellii strain DS40M4

39.594

98.01

0.388
  pilG Neisseria meningitidis 44/76-A

39.323

95.522

0.376

Multiple sequence alignment