Detailed information NTG0181610

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A7318_RS03330
• Genome accession: NZ_CP015639
• Coordinates: 770424..771290 (-)
• Length: 288 a.a.
• NCBI ID: WP_069021707.1
• Uniprot ID: -
• Organism: Pseudomonas lurida strain L228
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 765424..776290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A7318_RS03290 (A7318_03290)	-	765846..767144 (-)	1299	WP_069021698.1	NAD(P)/FAD-dependent oxidoreductase	-
A7318_RS03295 (A7318_03295)	-	767205..767378 (-)	174	WP_016976295.1	DUF3094 family protein	-
A7318_RS03300 (A7318_03300)	-	767433..768062 (-)	630	WP_010213242.1	DUF1780 domain-containing protein	-
A7318_RS03305 (A7318_03305)	-	768166..768633 (+)	468	WP_034102088.1	hypothetical protein	-
A7318_RS03310 (A7318_03310)	-	768630..769319 (+)	690	WP_069021700.1	energy-coupling factor ABC transporter permease	-
A7318_RS03315 (A7318_03315)	-	769395..769610 (+)	216	WP_069021702.1	hypothetical protein	-
A7318_RS03320 (A7318_03320)	yacG	769607..769807 (-)	201	WP_034102092.1	DNA gyrase inhibitor YacG	-
A7318_RS03325 (A7318_03325)	coaE	769804..770427 (-)	624	WP_069021704.1	dephospho-CoA kinase	-
A7318_RS03330 (A7318_03330)	pilD	770424..771290 (-)	867	WP_069021707.1	prepilin peptidase	Machinery gene
A7318_RS03335 (A7318_03335)	pilC	771287..772495 (-)	1209	WP_069021709.1	type II secretion system F family protein	Machinery gene
A7318_RS03340 (A7318_03340)	-	772698..773111 (+)	414	WP_069078682.1	pilin	-
A7318_RS03345 (A7318_03345)	-	773147..774547 (+)	1401	WP_069021711.1	O-antigen ligase family protein	-
A7318_RS03355 (A7318_03355)	-	774828..775133 (+)	306	WP_034102101.1	DUF6388 family protein	-
A7318_RS03360 (A7318_03360)	nadC	775208..776056 (-)	849	WP_069021713.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31812.03 Da
• Isoelectric Point: 8.1772

>NTDB_id=181610 A7318_RS03330 WP_069021707.1 770424..771290(-) (pilD) [Pseudomonas lurida strain L228]
MSLLLAGHPWAFIALASVLGLIVGSFLNVLVWRLPRMLEREWRAQAHEVLGLPAEPACPTYNLMHPNSCCPHCNHPIRPW
ENIPLLSYLFLRGRCAQCQKAISVRYPLTELACGLISAWVAWHFGFGWPAGAVLVLSWGLLAMSLIDADHQLLPDVLVMP
LLWLGLIVNGFGLLTTLPDALWGAVIGYMSLWSVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMSSLVGVF
VGLILMRLRKAQASAPMPFGPYLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=181610 A7318_RS03330 WP_069021707.1 770424..771290(-) (pilD) [Pseudomonas lurida strain L228]
TTGAGCCTGCTGCTGGCTGGCCACCCCTGGGCATTTATTGCGCTGGCGTCAGTGCTGGGACTGATCGTCGGCAGTTTTCT
CAACGTGCTGGTGTGGCGCCTGCCAAGGATGCTCGAACGGGAATGGCGTGCGCAGGCCCACGAAGTACTCGGCTTGCCCG
CTGAACCTGCCTGCCCGACCTACAACCTGATGCACCCCAACTCCTGCTGCCCGCATTGCAACCATCCGATACGCCCGTGG
GAAAACATCCCACTGCTCAGCTACCTGTTTCTGCGAGGCCGCTGCGCCCAATGCCAAAAGGCAATCAGCGTCCGTTATCC
GCTCACTGAACTGGCGTGCGGGTTGATCTCGGCCTGGGTTGCCTGGCATTTCGGCTTTGGCTGGCCAGCGGGCGCGGTGC
TGGTACTGAGTTGGGGGTTGTTGGCGATGAGCCTGATCGATGCCGATCACCAACTGTTGCCGGATGTGCTGGTGATGCCG
CTGCTGTGGCTAGGGCTGATCGTCAACGGGTTCGGACTGCTGACGACACTGCCCGATGCCCTGTGGGGCGCGGTGATCGG
GTACATGAGCCTGTGGAGCGTGTTCTGGCTGTTCAAGCTGGTTACCGGCAAGGACGGCATGGGCCATGGTGACTTCAAGC
TGCTGGCGCTGCTCGGTGCCTGGGGCGGCTGGCAGATTCTGCCGATGACGCTGTTGATGTCGTCACTGGTAGGTGTGTTC
GTGGGCTTGATCCTGATGCGCCTGCGCAAGGCCCAGGCATCGGCACCGATGCCATTTGGTCCGTATCTGGCGATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (134-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.763	96.875	0.521
pilD	Vibrio campbellii strain DS40M4	50	97.222	0.486
pilD	Neisseria gonorrhoeae MS11	51.111	93.75	0.479
pilD	Acinetobacter nosocomialis M2	49.084	94.792	0.465
pilD	Acinetobacter baumannii D1279779	48.352	94.792	0.458