Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AsACE_RS13270 Genome accession   NZ_CP015615
Coordinates   2736545..2738254 (+) Length   569 a.a.
NCBI ID   WP_076754535.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain ACE     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731545..2743254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AsACE_RS13225 (AsACE_CH02633) nusA 2732022..2733506 (-) 1485 WP_004810069.1 transcription termination factor NusA -
  AsACE_RS13230 (AsACE_CH02634) rimP 2733542..2734066 (-) 525 WP_076754530.1 ribosome maturation factor RimP -
  AsACE_RS13260 (AsACE_CH02640) secG 2735128..2735457 (-) 330 WP_004896327.1 preprotein translocase subunit SecG -
  AsACE_RS13265 (AsACE_CH02641) tpiA 2735471..2736262 (-) 792 WP_076754533.1 triose-phosphate isomerase -
  AsACE_RS13270 (AsACE_CH02642) pilB 2736545..2738254 (+) 1710 WP_076754535.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AsACE_RS13275 (AsACE_CH02643) pilC 2738286..2739515 (+) 1230 WP_004810078.1 type II secretion system F family protein Machinery gene
  AsACE_RS16670 (AsACE_CH02644) pilD 2739515..2740375 (+) 861 WP_076754537.1 prepilin peptidase Machinery gene
  AsACE_RS13285 (AsACE_CH02645) coaE 2740377..2740973 (+) 597 WP_076754540.1 dephospho-CoA kinase -
  AsACE_RS13290 (AsACE_CH02646) - 2740994..2741917 (-) 924 WP_034424053.1 DMT family transporter -
  AsACE_RS13295 (AsACE_CH02647) rlmB 2741924..2742673 (-) 750 WP_008306825.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AsACE_RS13300 (AsACE_CH02648) - 2742826..2743149 (-) 324 WP_004810087.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63554.72 Da        Isoelectric Point: 4.8758

>NTDB_id=181539 AsACE_RS13270 WP_076754535.1 2736545..2738254(+) (pilB) [Acinetobacter schindleri strain ACE]
MTAIQGSPRFTGFIRRLVDEGVISAEDMQGALSSAKKDKIDIVPYLIEHHRLSPTKIAETISLEFGEPLFDISAYDPALI
VREDFDEKIITKHRVLPIFKNSNILFIATSNPTNIEAIDAVRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDIDIDNSDPNKDDEDDNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKKSADIPRQSLLEMGFS
DDDLQNPEFNIFEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=181539 AsACE_RS13270 WP_076754535.1 2736545..2738254(+) (pilB) [Acinetobacter schindleri strain ACE]
ATGACAGCAATTCAGGGATCTCCAAGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGGGTTATATCTGCAGAAGA
TATGCAGGGTGCTTTGAGTAGTGCCAAAAAAGATAAAATTGATATTGTTCCCTATCTGATTGAACATCACCGCCTCTCGC
CAACCAAAATCGCAGAAACGATTTCTCTTGAATTCGGTGAGCCGCTATTTGACATCAGTGCTTATGATCCTGCCCTGATC
GTACGCGAAGATTTTGATGAAAAAATCATTACCAAACACCGCGTGCTACCAATCTTTAAAAATAGCAATATTCTGTTTAT
CGCGACCAGCAACCCGACCAATATAGAAGCAATTGATGCCGTTCGTTTTGCGACCAAACTCAATATTGAAACCATCATTG
TTGAACATAATAAACTCGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACCTTTGACTTTGATGATGATTTTGAT
CTAGATATTGATATCGATAACTCTGACCCGAATAAAGATGATGAGGATGATAATAAAGGTGATGAAGCACCCATTGTTAA
ATATATTAATAAGTTACTGATTGATGCAATCCGTATGGGTGCATCTGATTTACACTTTGAACCTTATGAAAAAATGTACC
GTGTGCGTTACCGTGTCGACGGGGTCTTGCGTCAAATCGCCACGCCACCTTTACAGCTTGCAACACGTCTGGCTTCACGT
CTTAAAGTTATGTCACAAATGGATATTTCCGAGAAGCGTGTGCCTCAAGATGGCCGGATCAAGTTAAAACTCTCTAAAAA
TAAGGCTATCGATTTCCGTGTCAACTCACTCCCAACCCTGTTTGGGGAAAAACTGGTACTGCGTATTCTGGATCCATCGA
GTGCCATGCTTGGAATTGATGCTCTGGGGTATGAGCCTGATCAAAAAGCCTTATTTATGGAGGCACTAGATAAGCCTCAA
GGTATGCTCTTGATCACCGGTCCGACAGGCTCTGGTAAAACTGTATCTCTCTATACCGGTTTAAATATCCTGAATCGTGA
AGACACGAATATTTCAACTGCTGAAGATCCAGTCGAAATTAACCTGCAAGGGATCAACCAGGTTAACGTCAATAATAAAG
TTGGCCTAACCTTCTCTGCTGCATTGAAATCATTCCTGCGCCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAC
TTGGAAACTGCAGAGATTGCAATCAAGGCAGCACAAACAGGTCATATGGTGATGTCAACACTACATACCAACAGCGCACC
AGAAACCCTGACCCGTTTACGTAATATGGGTGTGCCATCATTTAACATTGCCACCTCGGTGAATTTGGTTATTGCTCAGC
GTTTGGCGCGTCGCCTTTGTCCACATTGCAAAAAATCGGCAGATATTCCACGTCAGAGCCTGCTTGAAATGGGCTTTAGC
GATGATGATTTACAGAATCCGGAATTTAATATTTTTGAACCTGTCGGATGTAACGAATGTCGTGAAGGGTATAAAGGTCG
AGTCGGTATTTATGAAGTCATGAAAGTGACACCAGAGATTTCACGTATTATTATGGAAGATGGCAATGCAATTGAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.298

100

0.794
  pilB Acinetobacter baylyi ADP1

76.316

100

0.765
  pilB Legionella pneumophila strain ERS1305867

52.037

94.903

0.494
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio cholerae strain A1552

48.582

99.121

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.192

97.891

0.482
  pilB Vibrio campbellii strain DS40M4

47.935

97.891

0.469
  pilF Thermus thermophilus HB27

37.173

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

34.804

100

0.374

Multiple sequence alignment