Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A7J50_RS01985 Genome accession   NZ_CP015600
Coordinates   491273..492601 (+) Length   442 a.a.
NCBI ID   WP_064450308.1    Uniprot ID   -
Organism   Pseudomonas antarctica strain PAMC 27494     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486273..497601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7J50_RS01965 (A7J50_0395) - 486348..488810 (-) 2463 WP_407015984.1 penicillin-binding protein 1A -
  A7J50_RS01970 (A7J50_0396) pilM 489031..489687 (+) 657 WP_064454842.1 type IV pilus assembly protein PilM -
  A7J50_RS01975 (A7J50_0397) - 489684..490235 (+) 552 WP_064450306.1 PilN domain-containing protein -
  A7J50_RS01980 (A7J50_0398) - 490232..491203 (+) 972 WP_064450307.1 pilus assembly protein PilP -
  A7J50_RS01985 (A7J50_0399) pilQ 491273..492601 (+) 1329 WP_064450308.1 type IV pilus secretin PilQ Machinery gene
  A7J50_RS01990 (A7J50_0400) aroK 492606..493124 (+) 519 WP_064450309.1 shikimate kinase AroK -
  A7J50_RS01995 (A7J50_0401) aroB 493249..494349 (+) 1101 WP_053253955.1 3-dehydroquinate synthase -
  A7J50_RS02000 (A7J50_0402) - 494360..495934 (+) 1575 WP_064450310.1 AAA family ATPase -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 48197.21 Da        Isoelectric Point: 4.8912

>NTDB_id=181275 A7J50_RS01985 WP_064450308.1 491273..492601(+) (pilQ) [Pseudomonas antarctica strain PAMC 27494]
MAFAVPNRLDLIQLPPPGSSAVNTSYSGDKLNLNFQNIDLRAVLQQIADVAGLNLVASDDVQGSITLRLKEVPWDQALDL
VLQAKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTSLEGIPDERGSVA
VDERTNNIIAYQTGERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGKWGAGGINKPSAEGAEPP
ENPPSSPFVDMGSLTGTSGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSRGATS
VSFKEASLSLEVTPQITPDGWVVMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVP
FLGDVPYLGRLFRRDVLAERKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=181275 A7J50_RS01985 WP_064450308.1 491273..492601(+) (pilQ) [Pseudomonas antarctica strain PAMC 27494]
ATGGCATTTGCTGTGCCCAATCGCCTGGACCTGATCCAGCTGCCACCTCCCGGTAGCTCGGCGGTAAATACAAGCTATTC
CGGTGACAAGCTGAACCTCAACTTCCAGAACATCGATTTGCGCGCTGTATTGCAGCAGATTGCGGATGTCGCCGGGCTTA
ATCTGGTGGCCAGCGACGATGTGCAAGGCTCCATCACCCTGCGGCTCAAGGAGGTGCCCTGGGATCAGGCACTGGACCTG
GTGTTGCAAGCCAAGGGGTTGGACAAGCGGGTGAAGGCCGGTGTGTTGCTGGTGGCGCCGGCCGAAGAGTTGGCCGCCCG
CGAACTGCTGACCCTGGAGTCGCGCAAGCAAATGGCCGAGTTGGCGCCCTTGCGCCGGGAACTGTTGCAGGTCAATTACG
CCAAGGCGGCGGACCTGGCCAAATTGTTCCAATCAGTCACCAGCCTTGAAGGCATCCCCGATGAGCGCGGTTCGGTAGCC
GTGGATGAGCGCACCAATAACATTATTGCCTACCAGACCGGCGAACGGCTCGAAGAGCTGCGGCGGATCGTGGCGCAACT
GGATGTCCCGGTGCGCCAAGTGATGATCGAGGCGCGGATCGTTGAAGCCAATGTCGATTACGACAAAAGCCTTGGCGTGC
GCTGGGGTGGGCGCCTGAACCGTGGCAAGTGGGGGGCGGGCGGTATCAACAAACCTTCGGCCGAGGGTGCTGAACCGCCG
GAGAACCCGCCCAGCTCGCCGTTTGTCGACATGGGTTCGCTCACGGGTACTTCAGGCCTGGGTATCGCCTATATCACCGA
CAACCTGTTGCTGGATCTTGAGCTGACGGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCGCAGCCCAAGGTGGTCA
CCTCCGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATCCCCTATCAGGAATCCACCTCGCGCGGTGCTACATCG
GTGTCGTTCAAGGAAGCCTCGCTGTCGCTGGAGGTGACGCCGCAAATCACCCCGGATGGCTGGGTGGTCATGGAGGTCAA
GGTCACCAAGGACGAGCCCGACTATCTGAACAAGCTCAATGACGTACCGCCGATCAAGAAAAACGAAGTCAACGCCAAGG
TGCTGGTCAAGGATGGCGAGACCATCGTCATCGGGGGCGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCA
TTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAAAGAAAATCCGAGCTGCTGGTATT
CCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II and III secretion system protein (Secretin)

(277-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.843

99.321

0.704
  pilQ Acinetobacter baumannii D1279779

39.676

100

0.443
  pilQ Acinetobacter baumannii strain A118

39.676

100

0.443
  comQ Acinetobacter baylyi ADP1

40.833

100

0.443
  pilQ Vibrio campbellii strain DS40M4

43.735

95.701

0.419
  pilQ Legionella pneumophila strain Lp02

43.571

95.023

0.414
  pilQ Legionella pneumophila strain ERS1305867

43.095

95.023

0.41
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.029

94.118

0.405
  pilQ Vibrio cholerae strain A1552

43.029

94.118

0.405

Multiple sequence alignment