Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A3K93_RS11420 Genome accession   NZ_CP015594
Coordinates   2365762..2367471 (+) Length   569 a.a.
NCBI ID   WP_067731322.1    Uniprot ID   A0A172YR78
Organism   Acinetobacter sp. NCu2D-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2360762..2372471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3K93_RS11375 (A3K93_11375) nusA 2361343..2362827 (-) 1485 WP_067731318.1 transcription termination factor NusA -
  A3K93_RS11380 (A3K93_11380) rimP 2362863..2363387 (-) 525 WP_067731319.1 ribosome maturation factor RimP -
  A3K93_RS11410 (A3K93_11410) secG 2364343..2364672 (-) 330 WP_067731320.1 preprotein translocase subunit SecG -
  A3K93_RS11415 (A3K93_11415) tpiA 2364686..2365477 (-) 792 WP_067731321.1 triose-phosphate isomerase -
  A3K93_RS11420 (A3K93_11420) pilB 2365762..2367471 (+) 1710 WP_067731322.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3K93_RS11425 (A3K93_11425) pilC 2367505..2368734 (+) 1230 WP_067731323.1 type II secretion system F family protein Machinery gene
  A3K93_RS11430 (A3K93_11430) pilD 2368734..2369594 (+) 861 WP_067731324.1 A24 family peptidase Machinery gene
  A3K93_RS11435 (A3K93_11435) coaE 2369596..2370192 (+) 597 WP_067731325.1 dephospho-CoA kinase -
  A3K93_RS11440 (A3K93_11440) - 2370211..2371131 (-) 921 WP_081408536.1 DMT family transporter -
  A3K93_RS11445 (A3K93_11445) rlmB 2371165..2371914 (-) 750 WP_067731326.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  A3K93_RS11450 (A3K93_11450) - 2372055..2372378 (-) 324 WP_067731327.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63724.93 Da        Isoelectric Point: 4.8758

>NTDB_id=181258 A3K93_RS11420 WP_067731322.1 2365762..2367471(+) (pilB) [Acinetobacter sp. NCu2D-2]
MAAIQGTPRFTGFIRRIVDEGLLSAEHMQEALINSKRDKIDIVPYLIDFHKLSPTVIAENISTEFGEPLFDIGAYDPGQV
LKEEIDEKLITKHRILPIFKNSSILYIATSNPTNLEAMDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDIDVENPEANKDQEDDNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPHQSLLEMGFT
EEDLQQPDFQIYEPNGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNALQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=181258 A3K93_RS11420 WP_067731322.1 2365762..2367471(+) (pilB) [Acinetobacter sp. NCu2D-2]
ATGGCAGCAATTCAGGGGACTCCAAGATTTACAGGCTTTATTCGTCGTATTGTCGATGAAGGTCTTTTATCTGCAGAACA
TATGCAAGAAGCATTAATCAATTCTAAGCGCGATAAAATTGATATCGTGCCGTATTTGATTGACTTTCATAAGCTCTCCC
CGACTGTTATTGCTGAAAATATTTCAACTGAATTTGGTGAGCCACTATTTGATATTGGGGCATATGATCCAGGTCAAGTT
TTAAAAGAAGAAATCGATGAAAAATTAATTACTAAACATCGTATTTTACCGATTTTTAAAAATTCCAGTATTTTGTATAT
CGCAACCAGCAATCCAACCAATTTAGAGGCGATGGATGCAATACGTTTTGCGACAAAACTCAATATTGAAACCGTCATTG
TTGAGCACAATAAACTTGAAAAACTGATTGAGCAAAACTTTACCGAAGAAAGCACCTTTGATTTCGATGATGATTTTGAT
CTAGATATTGATGTCGAAAACCCTGAAGCCAATAAAGATCAGGAAGATGACAACAAAGGTGATGAAGCACCGATTGTTAA
ATACATCAATAAATTGCTGATTGATGCTATTCGTATGGGTGCATCGGATTTACACTTTGAACCTTATGAAAAAATTTACC
GTGTGCGTTACCGTGTCGATGGTGTACTGCGTCAAATCGCGACACCGCCACTACAGTTAGCGACTCGCCTATCTTCACGT
TTAAAAGTCATGTCACAAATGGACATTTCTGAAAAACGTGTTCCTCAAGATGGTCGTATTAAACTTAAACTGTCGAAAAA
CAAAGCCATCGATTTCCGTGTCAACTCATTGCCAACACTATTTGGTGAAAAACTAGTATTGCGTATTCTCGACCCATCGA
GTGCAATGCTTGGGATTGATGCTCTTGGTTATGAGCCTGATCAGAAAGAACTCTTCATGCAGGCGCTTGAAAAACCTCAA
GGCATGCTATTAATTACAGGTCCAACAGGTTCAGGTAAGACCGTATCTTTATATACGGGTCTTAACATTTTAAACCGTGA
AGATACCAATATTTCAACGGCGGAAGACCCAGTCGAAATTAACCTACAAGGGATTAATCAGGTCAACGTAAACAATAAAG
TCGGCCTGACTTTCTCTGCTGCATTGAAATCTTTCTTGCGTCAGGACCCTGATATCGTCATGGTCGGTGAGATTCGTGAT
TTAGAGACTGCTGAAATTGCAGTGAAAGCCGCTCAAACCGGTCATATGGTGATGTCAACACTGCATACCAACAGTGCACC
TGAAACTTTGACCCGTTTACGCAATATGGGTGTGCCCTCTTTTAATATTGCAACTTCGGTGAACTTGGTGATTGCACAGC
GTCTTGCCCGTCGTCTTTGCTCACAATGTAAAAAGCCCGCTGATATTCCACATCAAAGTTTGCTGGAAATGGGCTTCACT
GAAGAAGATTTACAACAACCCGATTTTCAAATTTATGAGCCAAATGGCTGTAGTGAATGCCGTGAAGGCTATAAAGGCCG
AGTAGGTATTTATGAAGTCATGAAAGTAACTCCAGAAATTTCAAGAATTATTATGGAAGATGGCAATGCATTACAAATTG
CCGAAGCTTCCGCACGTGCAGGTTTTAATAATTTACGCCGATCAGGTTTATTGAAGGTTATGCAGGGGGTGACTTCTCTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172YR78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.298

100

0.794
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

51.237

99.473

0.51
  pilF Neisseria gonorrhoeae MS11

48.932

98.77

0.483
  pilB Vibrio cholerae strain A1552

49.094

97.012

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.732

97.012

0.473
  pilB Vibrio campbellii strain DS40M4

48.463

97.188

0.471
  pilF Thermus thermophilus HB27

37.478

100

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

33.605

100

0.362

Multiple sequence alignment