Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC36_RS12700 Genome accession   NZ_CP015512
Coordinates   2650020..2651708 (+) Length   562 a.a.
NCBI ID   WP_045596841.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_036     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2645020..2656708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC36_RS12680 (FORC36_2385) pdhR 2646622..2647389 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FORC36_RS12685 (FORC36_2386) ampD 2647778..2648350 (-) 573 WP_013571143.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC36_RS12690 (FORC36_2387) nadC 2648443..2649330 (+) 888 WP_013571142.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC36_RS12695 (FORC36_2388) pilA 2649553..2650020 (+) 468 WP_045596840.1 pilin Machinery gene
  FORC36_RS12700 (FORC36_2389) pilB 2650020..2651708 (+) 1689 WP_045596841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC36_RS12705 (FORC36_2390) pilC 2651757..2652983 (+) 1227 WP_085761331.1 type II secretion system F family protein Machinery gene
  FORC36_RS12710 (FORC36_2391) pilD 2653077..2653946 (+) 870 WP_011079548.1 prepilin peptidase Machinery gene
  FORC36_RS12715 (FORC36_2392) coaE 2653948..2654556 (+) 609 WP_045596843.1 dephospho-CoA kinase -
  FORC36_RS12720 (FORC36_2393) zapD 2654589..2655329 (+) 741 WP_045596844.1 cell division protein ZapD -
  FORC36_RS12725 (FORC36_2394) yacG 2655392..2655586 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  FORC36_RS12730 (FORC36_2395) rplS 2655788..2656141 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62412.37 Da        Isoelectric Point: 5.5213

>NTDB_id=180217 FORC36_RS12700 WP_045596841.1 2650020..2651708(+) (pilB) [Vibrio vulnificus strain FORC_036]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQDLCNQLGLRE
LITHHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKAED
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=180217 FORC36_RS12700 WP_045596841.1 2650020..2651708(+) (pilB) [Vibrio vulnificus strain FORC_036]
ATGCAATCCAACCTTGCCACGATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAGTTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGTACTGACCTCAACCAATATGACTACCAAGATCTGTGTAATCAACTTGGGTTACGTGAG
CTAATCACCCATCACCAAGCATTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCTGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGTCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTTGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATCCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCTGCGAGGATAAAG
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGTGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAACAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATTTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTCGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGCCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCCTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCCACATTTGAGTTACTCGGTCTAAAAGCCGAAGAC
TTAATTTATCAAGCCGATAAACAAGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGTGCCTCGGTACAGAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.335

100

0.763
  pilB Vibrio parahaemolyticus RIMD 2210633

75.801

100

0.758
  pilB Vibrio cholerae strain A1552

74.245

100

0.744
  pilB Legionella pneumophila strain ERS1305867

52.632

91.281

0.48
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

50.575

92.883

0.47
  pilF Neisseria gonorrhoeae MS11

45.487

100

0.457
  pilF Thermus thermophilus HB27

41.25

99.644

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.302

94.306

0.361

Multiple sequence alignment