Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC53_RS02920 Genome accession   NZ_CP019290
Coordinates   584381..584821 (-) Length   146 a.a.
NCBI ID   WP_118893302.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579381..589821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC53_RS02900 (FORC53_0508) coaE 579830..580438 (-) 609 WP_017789873.1 dephospho-CoA kinase -
  FORC53_RS02905 (FORC53_0509) pilD 580440..581309 (-) 870 WP_118893301.1 prepilin peptidase Machinery gene
  FORC53_RS02910 (FORC53_0510) pilC 581403..582629 (-) 1227 WP_045618629.1 type II secretion system F family protein Machinery gene
  FORC53_RS02915 (FORC53_0511) pilB 582677..584365 (-) 1689 WP_118894060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC53_RS02920 (FORC53_0512) pilA 584381..584821 (-) 441 WP_118893302.1 pilin Machinery gene
  FORC53_RS02925 (FORC53_0513) nadC 585044..585931 (-) 888 WP_065090099.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC53_RS02930 (FORC53_0514) ampD 586024..586596 (+) 573 WP_072608922.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC53_RS02935 (FORC53_0515) pdhR 586985..587752 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15012.20 Da        Isoelectric Point: 9.9585

>NTDB_id=179743 FORC53_RS02920 WP_118893302.1 584381..584821(-) (pilA) [Vibrio vulnificus strain FORC_053]
MKKLNKTKKQQGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALASITGHRINVETYVVENGTFPTSAQLAVPTS
PLGTITYTAAASGASASSGAIKFSFGSTGVSPDVLNKDVTLNRDGSGKWSCTSGITADGVRPKGCS

Nucleotide


Download         Length: 441 bp        

>NTDB_id=179743 FORC53_RS02920 WP_118893302.1 584381..584821(-) (pilA) [Vibrio vulnificus strain FORC_053]
ATGAAGAAATTGAACAAAACCAAGAAACAACAAGGTTTTACCTTGATTGAATTAATGATAGTGGTAGCGGTAATTGGCGT
ACTCGCGGCAATTGCAGTACCCCAATATCAAAAGTACGTTGCCAAAAGTGAAGCCGCTTCAGCTTTGGCGTCAATCACAG
GACACAGAATAAATGTTGAAACATATGTTGTGGAGAATGGAACATTTCCAACATCAGCTCAGCTAGCAGTTCCAACTTCA
CCCTTAGGCACGATTACTTATACGGCTGCCGCATCAGGAGCTAGCGCATCTTCAGGAGCTATTAAGTTCTCGTTTGGTTC
GACAGGAGTTAGCCCTGATGTATTAAACAAAGATGTAACTTTGAATAGAGATGGTTCCGGAAAATGGTCTTGTACTTCTG
GTATTACAGCCGATGGAGTTAGGCCAAAAGGCTGTTCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(41-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.033

100

0.466
  pilA Vibrio cholerae strain A1552

45.033

100

0.466
  pilA Vibrio cholerae C6706

45.033

100

0.466
  pilA Pseudomonas aeruginosa PAK

43.421

100

0.452
  pilA Vibrio parahaemolyticus RIMD 2210633

42.282

100

0.432
  pilA Acinetobacter baumannii strain A118

41.096

100

0.411
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.096

100

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.028

97.26

0.37