Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FORC53_RS01930 Genome accession   NZ_CP019290
Coordinates   397526..399250 (+) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain FORC_053     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 392526..404250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC53_RS01905 (FORC53_0337) gspM 392930..393580 (+) 651 WP_011079392.1 type II secretion system protein GspM -
  FORC53_RS01910 (FORC53_0338) - 393573..393893 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  FORC53_RS01915 (FORC53_0339) mshL 393909..395555 (+) 1647 WP_118893246.1 pilus (MSHA type) biogenesis protein MshL -
  FORC53_RS01920 (FORC53_0340) - 395563..396411 (+) 849 WP_118893248.1 ExeA family protein -
  FORC53_RS01925 (FORC53_0341) - 396408..397529 (+) 1122 WP_103199897.1 tetratricopeptide repeat protein -
  FORC53_RS01930 (FORC53_0342) pilF 397526..399250 (+) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  FORC53_RS01935 (FORC53_0343) - 399260..400480 (+) 1221 WP_011079398.1 type II secretion system F family protein -
  FORC53_RS01940 (FORC53_0344) - 400484..400945 (+) 462 WP_039554781.1 hypothetical protein -
  FORC53_RS01945 (FORC53_0345) - 401024..401602 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC53_RS01950 (FORC53_0346) - 401636..402145 (+) 510 WP_013571064.1 type II secretion system protein -
  FORC53_RS01955 (FORC53_0347) - 402166..402660 (+) 495 WP_118893250.1 type II secretion system protein -
  FORC53_RS01960 (FORC53_0348) - 402660..403226 (+) 567 WP_118893252.1 type IV pilus modification PilV family protein -
  FORC53_RS01965 (FORC53_0349) - 403226..403915 (+) 690 WP_118893254.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=179731 FORC53_RS01930 WP_011079397.1 397526..399250(+) (pilF) [Vibrio vulnificus strain FORC_053]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=179731 FORC53_RS01930 WP_011079397.1 397526..399250(+) (pilF) [Vibrio vulnificus strain FORC_053]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCGCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAGTTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGCGATGAAGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCATCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGTTTGCGCCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCCTTGGTACTGAGATTAAAACTCATG
GCCAACTTGGACATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTG
ACTGGCCCTACGGGCTCCGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTCTCGCGTGTCAATCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACATTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACGGAAGATCATGTTGGCGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCAAATCAAGTG
GAAGCGGGTTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCACATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAACTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment