Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A6044_RS03760 Genome accession   NZ_CP015432
Coordinates   703213..704607 (+) Length   464 a.a.
NCBI ID   WP_064088541.1    Uniprot ID   -
Organism   [Haemophilus] ducreyi strain GHA5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698213..709607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6044_RS03730 (A6044_03740) nth 698756..699391 (-) 636 WP_010945029.1 endonuclease III -
  A6044_RS03735 (A6044_03745) - 699421..700170 (-) 750 WP_041603673.1 TSUP family transporter -
  A6044_RS03740 (A6044_03750) - 700290..700775 (+) 486 WP_010945031.1 dihydrofolate reductase -
  A6044_RS03745 (A6044_03755) - 700897..701112 (+) 216 WP_010945033.1 YgjV family protein -
  A6044_RS03750 (A6044_03760) radA 701179..702558 (-) 1380 WP_010945034.1 DNA repair protein RadA -
  A6044_RS03755 (A6044_03765) pilA 702752..703201 (+) 450 WP_010945035.1 prepilin peptidase-dependent pilin Machinery gene
  A6044_RS03760 (A6044_03770) pilB 703213..704607 (+) 1395 WP_064088541.1 GspE/PulE family protein Machinery gene
  A6044_RS03765 (A6044_03775) pilC 704591..705784 (+) 1194 WP_010945037.1 type II secretion system F family protein Machinery gene
  A6044_RS03770 (A6044_03780) - 705784..706476 (+) 693 WP_041603480.1 prepilin peptidase -
  A6044_RS03775 (A6044_03785) coaE 706494..707111 (+) 618 WP_010945039.1 dephospho-CoA kinase -
  A6044_RS03780 (A6044_03790) yacG 707114..707302 (+) 189 WP_010945040.1 DNA gyrase inhibitor YacG -
  A6044_RS03785 (A6044_03795) holA 707336..708361 (-) 1026 WP_010945041.1 DNA polymerase III subunit delta -
  A6044_RS03790 (A6044_03800) lptE 708367..708864 (-) 498 WP_010945042.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52917.03 Da        Isoelectric Point: 6.6454

>NTDB_id=179601 A6044_RS03760 WP_064088541.1 703213..704607(+) (pilB) [[Haemophilus] ducreyi strain GHA5]
MQYAVFDLHRQQNFIISAERWQKNCQEKDLLLRYLALPVQENEDRLWLAIDDMKNLNACEIFAFMAHKTIEPVLVSTEEL
KYLLNQLAPEQQAVYEESELTYQVQTESETLNLNDPIIQLLDNLFKFCLRQNASDIHFEPQKAQLQIRLRIDGVLHIYKT
LAIHLAARIISRIKLLARLDISETRLPQDGQFHFKTIVSETLDFRVSSLPTQWGGKIVLRLQKNKPTHFDFVELGSLPAQ
KTTLLHLLHQPQGLILVTGPTGSGKSITLYSALSHLNQPDKHILTAEDPIEIEIEGIIQTQVNRAIQLDFSHLLRTFLRQ
DPDIIMLGEIRDPESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELKNALLLVIAQRLVRKLCTQCFGKGC
QACYQGYRGRIGIYQLLSRTGKIFEKETAYLDFASLHHSAKHKLEADITSLAEINRVLGDAENI

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=179601 A6044_RS03760 WP_064088541.1 703213..704607(+) (pilB) [[Haemophilus] ducreyi strain GHA5]
ATGCAGTATGCGGTATTTGATCTTCATCGCCAACAGAATTTTATTATTTCAGCGGAGAGATGGCAAAAGAACTGTCAAGA
AAAAGATCTTTTATTACGTTATCTTGCATTACCGGTTCAAGAAAATGAGGACAGGTTATGGCTAGCCATTGATGATATGA
AAAATTTAAATGCGTGTGAAATTTTTGCATTTATGGCTCATAAAACGATAGAACCGGTGTTGGTTTCTACGGAAGAATTG
AAATATTTATTGAATCAACTGGCGCCAGAGCAACAAGCGGTATATGAAGAAAGTGAGCTAACTTATCAAGTACAAACAGA
AAGTGAAACATTGAATTTAAATGATCCTATTATTCAATTACTTGATAATTTATTTAAATTTTGTTTAAGGCAAAATGCTT
CCGATATTCATTTTGAGCCACAAAAAGCACAGTTACAGATTCGGTTGCGGATTGATGGTGTGTTACATATCTACAAAACA
TTGGCAATTCATTTGGCTGCTCGCATTATTTCACGTATCAAATTGTTGGCTAGACTTGATATTAGCGAAACACGGCTACC
GCAAGATGGGCAATTCCATTTTAAAACAATCGTATCTGAAACCCTTGATTTCCGTGTATCGAGTTTGCCTACTCAATGGG
GCGGAAAAATTGTATTACGCTTACAAAAAAATAAACCGACCCATTTTGATTTTGTTGAATTGGGGTCATTGCCTGCGCAA
AAAACTACGTTATTACACTTATTGCATCAACCACAAGGTCTAATTTTAGTTACTGGCCCCACCGGCAGTGGCAAAAGTAT
CACATTGTATAGTGCATTAAGTCATCTGAATCAACCGGATAAGCATATTTTAACGGCAGAAGATCCAATAGAAATTGAGA
TCGAAGGAATCATTCAAACACAAGTTAATCGAGCGATTCAGCTAGATTTTAGCCATCTATTACGAACATTTTTGCGCCAA
GATCCTGATATTATTATGTTAGGTGAGATTCGTGATCCGGAGAGTGCCGAAATTGCATTACGCGCTGCACAAACAGGGCA
TCTTGTGCTTTCTACATTACATACTAATGATGCGCCCTCTGCAATTGAACGGCTATTACAATTAGGTATTAAAGAATATG
AGCTTAAAAACGCCTTATTATTAGTCATTGCACAACGGCTTGTCCGCAAGCTATGCACGCAATGTTTTGGTAAAGGCTGT
CAGGCTTGCTATCAGGGCTATAGAGGACGCATTGGCATTTATCAATTATTAAGCCGTACGGGAAAAATCTTTGAAAAAGA
GACCGCTTACCTTGATTTTGCTAGTTTACATCATAGTGCGAAGCATAAGTTAGAGGCTGATATTACGTCCTTAGCAGAAA
TTAACAGAGTATTAGGCGATGCTGAAAATATATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

74.017

98.707

0.731
  pilB Haemophilus influenzae Rd KW20

58.482

96.552

0.565
  pilB Haemophilus influenzae 86-028NP

57.906

96.767

0.56
  pilB Vibrio campbellii strain DS40M4

40.408

100

0.427
  pilB Vibrio cholerae strain A1552

39.919

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

38.386

100

0.42
  pilB Legionella pneumophila strain ERS1305867

38.821

100

0.412
  pilB Acinetobacter baylyi ADP1

38.73

100

0.407
  pilB Acinetobacter baumannii D1279779

37.654

100

0.394
  pilF Neisseria gonorrhoeae MS11

36.076

100

0.369

Multiple sequence alignment