Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6044_RS03755 Genome accession   NZ_CP015432
Coordinates   702752..703201 (+) Length   149 a.a.
NCBI ID   WP_010945035.1    Uniprot ID   -
Organism   [Haemophilus] ducreyi strain GHA5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697752..708201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6044_RS08635 - 697881..697991 (-) 111 Protein_725 formate dehydrogenase N subunit beta transmembrane domain-containing protein -
  A6044_RS03725 (A6044_03735) fdhD 697992..698678 (+) 687 Protein_726 formate dehydrogenase accessory sulfurtransferase FdhD -
  A6044_RS03730 (A6044_03740) nth 698756..699391 (-) 636 WP_010945029.1 endonuclease III -
  A6044_RS03735 (A6044_03745) - 699421..700170 (-) 750 WP_041603673.1 TSUP family transporter -
  A6044_RS03740 (A6044_03750) - 700290..700775 (+) 486 WP_010945031.1 dihydrofolate reductase -
  A6044_RS03745 (A6044_03755) - 700897..701112 (+) 216 WP_010945033.1 YgjV family protein -
  A6044_RS03750 (A6044_03760) radA 701179..702558 (-) 1380 WP_010945034.1 DNA repair protein RadA -
  A6044_RS03755 (A6044_03765) pilA 702752..703201 (+) 450 WP_010945035.1 prepilin peptidase-dependent pilin Machinery gene
  A6044_RS03760 (A6044_03770) pilB 703213..704607 (+) 1395 WP_064088541.1 GspE/PulE family protein Machinery gene
  A6044_RS03765 (A6044_03775) pilC 704591..705784 (+) 1194 WP_010945037.1 type II secretion system F family protein Machinery gene
  A6044_RS03770 (A6044_03780) - 705784..706476 (+) 693 WP_041603480.1 prepilin peptidase -
  A6044_RS03775 (A6044_03785) coaE 706494..707111 (+) 618 WP_010945039.1 dephospho-CoA kinase -
  A6044_RS03780 (A6044_03790) yacG 707114..707302 (+) 189 WP_010945040.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16256.69 Da        Isoelectric Point: 6.9012

>NTDB_id=179600 A6044_RS03755 WP_010945035.1 702752..703201(+) (pilA) [[Haemophilus] ducreyi strain GHA5]
MSSLFLIKPLQKAFTLIELMIVIAIIAILATVAIPSYNSYTKKAALSELLAASSAYKTDVEICVYNLGRTDGCNNGENGI
KNDKLDKSKYVASVAVENGEITVKGKESLKDYSYTMKPELQQDGHLTWKTTCNGADTSLFPANFCSTKE

Nucleotide


Download         Length: 450 bp        

>NTDB_id=179600 A6044_RS03755 WP_010945035.1 702752..703201(+) (pilA) [[Haemophilus] ducreyi strain GHA5]
ATGTCATCTTTATTTTTGATTAAGCCACTACAAAAAGCGTTTACTTTAATTGAATTAATGATTGTGATTGCCATTATCGC
AATTTTAGCCACGGTTGCGATTCCTTCTTACAATAGTTATACCAAAAAAGCTGCGTTATCTGAGTTATTAGCCGCTTCAT
CAGCGTATAAAACCGATGTAGAAATATGTGTTTATAATCTTGGTAGAACGGATGGATGCAATAATGGAGAAAATGGTATA
AAAAATGATAAATTAGATAAAAGTAAATATGTTGCTTCAGTTGCGGTGGAAAATGGAGAAATTACAGTAAAAGGTAAAGA
AAGCCTTAAAGATTATAGTTACACAATGAAGCCAGAACTACAACAAGATGGGCACTTAACTTGGAAAACGACTTGTAATG
GTGCTGATACTAGCCTTTTCCCTGCTAATTTTTGTTCTACTAAAGAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)

Pilin (bacterial filament) (Pilin)

(43-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

59.868

100

0.611
  pilA Haemophilus influenzae Rd KW20

56.25

96.644

0.544
  pilA Haemophilus influenzae 86-028NP

57.143

93.96

0.537

Multiple sequence alignment