Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A6R75_RS03230 Genome accession   NZ_CP015377
Coordinates   681341..682558 (+) Length   405 a.a.
NCBI ID   WP_023092874.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain BAMCPA07-48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 676341..687558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6R75_RS03205 (A6R75_03205) nadC 676444..677292 (+) 849 WP_023092870.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A6R75_RS03215 (A6R75_03215) - 677473..678786 (-) 1314 WP_023980542.1 O-antigen ligase family protein -
  A6R75_RS03220 (A6R75_03220) - 678942..679406 (-) 465 WP_023092872.1 pilin -
  A6R75_RS03225 (A6R75_03225) pilB 679637..681337 (+) 1701 WP_023092873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A6R75_RS03230 (A6R75_03230) pilC 681341..682558 (+) 1218 WP_023092874.1 type II secretion system F family protein Machinery gene
  A6R75_RS03235 (A6R75_03235) pilD 682559..683431 (+) 873 WP_023092875.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  A6R75_RS03240 (A6R75_03240) coaE 683428..684039 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  A6R75_RS03245 (A6R75_03245) yacG 684036..684236 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  A6R75_RS03250 (A6R75_03250) - 684273..684482 (-) 210 WP_003094660.1 hypothetical protein -
  A6R75_RS03255 (A6R75_03255) - 684588..685277 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  A6R75_RS03260 (A6R75_03260) - 685274..685744 (-) 471 WP_003094664.1 hypothetical protein -
  A6R75_RS03265 (A6R75_03265) - 685741..686166 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  A6R75_RS03270 (A6R75_03270) - 686299..686928 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  A6R75_RS03275 (A6R75_03275) - 686925..687374 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44028.83 Da        Isoelectric Point: 9.8103

>NTDB_id=178966 A6R75_RS03230 WP_023092874.1 681341..682558(+) (pilC) [Pseudomonas aeruginosa strain BAMCPA07-48]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVASGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=178966 A6R75_RS03230 WP_023092874.1 681341..682558(+) (pilC) [Pseudomonas aeruginosa strain BAMCPA07-48]
ATGGCGGACAAAGCGTTAAAAACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TTTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCACTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCAGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCAATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTTCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTTTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
TTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

37.905

99.012

0.375

Multiple sequence alignment