Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A6K25_RS09445 Genome accession   NZ_CP015345
Coordinates   2281213..2282421 (-) Length   402 a.a.
NCBI ID   WP_057789976.1    Uniprot ID   A0AAW7Z4S8
Organism   Alteromonas stellipolaris strain PQQ-42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2276213..2287421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6K25_RS09415 (A6K25_09430) mutT 2276958..2277353 (+) 396 WP_057789965.1 8-oxo-dGTP diphosphatase MutT -
  A6K25_RS09420 (A6K25_09435) - 2277461..2278339 (-) 879 WP_057789967.1 aspartoacylase -
  A6K25_RS09425 (A6K25_09440) yacG 2278350..2278577 (-) 228 WP_057789970.1 DNA gyrase inhibitor YacG -
  A6K25_RS09430 (A6K25_09445) zapD 2278619..2279371 (-) 753 WP_057789972.1 cell division protein ZapD -
  A6K25_RS09435 (A6K25_09450) coaE 2279538..2280122 (-) 585 WP_057795957.1 dephospho-CoA kinase -
  A6K25_RS09440 (A6K25_09455) pilD 2280182..2281102 (-) 921 WP_057789974.1 prepilin peptidase Machinery gene
  A6K25_RS09445 (A6K25_09460) pilC 2281213..2282421 (-) 1209 WP_057789976.1 type II secretion system F family protein Machinery gene
  A6K25_RS09450 (A6K25_09465) - 2282487..2282936 (-) 450 WP_057789978.1 pilin -
  A6K25_RS09455 (A6K25_09470) nadC 2283302..2284156 (-) 855 WP_057789980.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A6K25_RS09460 (A6K25_09475) - 2284175..2284705 (-) 531 WP_138118214.1 retropepsin-like aspartic protease family protein -
  A6K25_RS09465 (A6K25_09480) ampD 2285162..2285734 (+) 573 WP_063456813.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A6K25_RS09470 (A6K25_09485) ampE 2285794..2286663 (+) 870 WP_057789984.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43781.43 Da        Isoelectric Point: 9.6005

>NTDB_id=178634 A6K25_RS09445 WP_057789976.1 2281213..2282421(-) (pilC) [Alteromonas stellipolaris strain PQQ-42]
MAKATLVFEWQGKDRKGNSQKGELSAINIAEAKSLLRRRGISANRVRKQSASLFKRKQKIKAADISIVSRQIATMLSAGV
SLIQSLEMISQGHANPAARKLLSEITDEVRAGNPLSSALRKHPDYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVVVVALIVTTILLIFVVPQFEEIFSSFGAELPGFTLLVLGISNFVQSYGIFIGICISVAGYLFMRAH
KKSQSFRDRVDRAVLKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVFRDAILYIRKEVAGGMPMF
ISMRATNVFPDMVTQMVAIGEESGSVEEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIIAMYLPIFEMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=178634 A6K25_RS09445 WP_057789976.1 2281213..2282421(-) (pilC) [Alteromonas stellipolaris strain PQQ-42]
ATGGCAAAGGCAACATTAGTATTTGAATGGCAGGGGAAAGATAGAAAAGGTAATAGCCAAAAAGGCGAACTCTCCGCTAT
CAACATTGCTGAAGCTAAATCTCTACTGCGCCGAAGAGGAATTTCCGCCAACAGGGTTAGGAAACAAAGCGCCTCACTGT
TTAAGCGTAAACAAAAAATCAAAGCTGCAGATATATCGATTGTATCTCGCCAAATTGCAACAATGCTTTCAGCGGGCGTT
TCTTTAATTCAGTCCTTAGAAATGATTTCACAGGGTCATGCCAATCCAGCAGCCCGGAAACTCCTATCGGAGATAACTGA
TGAAGTTCGCGCAGGTAATCCACTTTCTTCAGCGCTACGTAAACATCCAGACTATTTTGATGATTTATACTGTGACTTAG
TTTATACCGGTGAACAATCCGGTGCTTTAGAAACTATTTACGACCGAATAGCCACTTATAAAGAAAAAGCGGAAGCCCTT
AAATCTAAAATCAAAAAGGCCATGTTTTATCCTATTGCTGTGGTTGTTGTAGCGCTTATTGTAACAACCATATTATTGAT
TTTCGTAGTTCCTCAGTTTGAAGAAATATTTAGCAGCTTTGGTGCGGAACTCCCCGGATTCACTCTATTAGTACTGGGAA
TTTCAAATTTTGTCCAAAGTTATGGGATATTCATAGGCATTTGCATTAGTGTAGCTGGCTACTTGTTTATGCGGGCTCAT
AAGAAAAGCCAAAGCTTTCGCGATAGAGTTGATAGAGCGGTTCTTAAAATTCCCGTTATTGGGGAAATACTCAAAAAAGC
CAGTATCGCAAGATTTACTCGCACCCTCGCAACTACTTTTGCTGCCGGTGTACCTTTAATTGGCGCACTTGAATCGGCTG
CTGGCGCGTCGGGCAATGCGGTATTTAGAGACGCTATCTTATATATTAGAAAAGAAGTGGCTGGCGGTATGCCAATGTTT
ATCTCCATGCGCGCCACCAATGTATTTCCCGATATGGTCACGCAGATGGTCGCCATTGGTGAAGAATCTGGCTCGGTTGA
GGAAATGCTCAGTAAAATAGCAACAATATATGAAGCCGAAGTAGATGATATGGTCGACGGCTTAACCAGCTTGCTTGAGC
CAATGATCATGGCTGTGCTAGGTGTGGTGATTGGTGGACTTATTATCGCCATGTACCTTCCCATATTTGAAATGGGTAAT
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.101

98.507

0.592
  pilC Acinetobacter baylyi ADP1

53.518

99.005

0.53
  pilC Acinetobacter baumannii D1279779

52.897

98.756

0.522
  pilC Legionella pneumophila strain ERS1305867

50.754

99.005

0.502
  pilC Vibrio cholerae strain A1552

46.212

98.507

0.455
  pilC Vibrio campbellii strain DS40M4

43.86

99.254

0.435
  pilG Neisseria meningitidis 44/76-A

44.241

95.025

0.42
  pilG Neisseria gonorrhoeae MS11

44.241

95.025

0.42

Multiple sequence alignment