Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IEC338SC_RS03970 Genome accession   NZ_CP015145
Coordinates   823497..825209 (-) Length   570 a.a.
NCBI ID   WP_063098008.1    Uniprot ID   -
Organism   Acinetobacter pittii strain IEC338SC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818497..830209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEC338SC_RS03940 (IEC338SC_0793) - 818661..818987 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  IEC338SC_RS03945 (IEC338SC_0794) rlmB 819090..819839 (+) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IEC338SC_RS03950 (IEC338SC_0795) - 819875..820789 (+) 915 WP_063098004.1 DMT family transporter -
  IEC338SC_RS03955 (IEC338SC_0796) coaE 820786..821382 (-) 597 WP_063098005.1 dephospho-CoA kinase -
  IEC338SC_RS03960 (IEC338SC_0797) pilD 821384..822244 (-) 861 WP_063098006.1 prepilin peptidase Machinery gene
  IEC338SC_RS03965 (IEC338SC_0798) pilC 822244..823470 (-) 1227 WP_063098007.1 type II secretion system F family protein Machinery gene
  IEC338SC_RS03970 (IEC338SC_0799) pilB 823497..825209 (-) 1713 WP_063098008.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IEC338SC_RS03975 (IEC338SC_0800) tpiA 825501..826295 (+) 795 WP_005079338.1 triose-phosphate isomerase -
  IEC338SC_RS03980 (IEC338SC_0801) secG 826308..826637 (+) 330 WP_002115012.1 preprotein translocase subunit SecG -
  IEC338SC_RS04000 (IEC338SC_0805) rimP 827275..827799 (+) 525 WP_002114922.1 ribosome maturation factor RimP -
  IEC338SC_RS04005 (IEC338SC_0806) nusA 827837..829321 (+) 1485 WP_002115134.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63582.06 Da        Isoelectric Point: 5.0294

>NTDB_id=177098 IEC338SC_RS03970 WP_063098008.1 823497..825209(-) (pilB) [Acinetobacter pittii strain IEC338SC]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDISQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDVIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=177098 IEC338SC_RS03970 WP_063098008.1 823497..825209(-) (pilB) [Acinetobacter pittii strain IEC338SC]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATATAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCGAGTAATATTGAAGCGATTGATGTCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTCATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGCAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTATCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTACCTCAAGACGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCCATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTCTAATTACAGGCCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCTGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGATCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCAACACTGCATACCAATAGTGC
TCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTGATTGCAC
AGCGTTTAGCACGTCGGCTATGCCCACAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTCGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

77.895

100

0.779
  pilB Legionella pneumophila strain ERS1305867

51.499

99.474

0.512
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

91.93

0.475
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.4

100

0.381
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment