Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A4G16_RS05270 Genome accession   NZ_CP015030
Coordinates   1095020..1096414 (-) Length   464 a.a.
NCBI ID   WP_165889002.1    Uniprot ID   A0A6G8JI73
Organism   Mannheimia granulomatis strain B 234/94     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1090020..1101414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G16_RS05230 (A4G16_05120) lptE 1090082..1090576 (+) 495 WP_165888994.1 LPS assembly lipoprotein LptE -
  A4G16_RS05235 (A4G16_05125) holA 1090619..1091644 (+) 1026 WP_165888995.1 DNA polymerase III subunit delta -
  A4G16_RS05240 (A4G16_05130) yacG 1091646..1091831 (-) 186 WP_165888996.1 DNA gyrase inhibitor YacG -
  A4G16_RS05245 (A4G16_05135) coaE 1091816..1092451 (-) 636 WP_165888997.1 dephospho-CoA kinase -
  A4G16_RS05250 (A4G16_05140) - 1092460..1092771 (-) 312 WP_165888998.1 helix-turn-helix domain-containing protein -
  A4G16_RS05255 (A4G16_05145) - 1092772..1093149 (-) 378 WP_165888999.1 type II toxin-antitoxin system RelE/ParE family toxin -
  A4G16_RS05260 (A4G16_05150) - 1093192..1093827 (-) 636 WP_165889000.1 prepilin peptidase -
  A4G16_RS05265 (A4G16_05155) pilC 1093831..1095027 (-) 1197 WP_165889001.1 type II secretion system F family protein Machinery gene
  A4G16_RS05270 (A4G16_05160) pilB 1095020..1096414 (-) 1395 WP_165889002.1 GspE/PulE family protein Machinery gene
  A4G16_RS05275 (A4G16_05165) pilA 1096437..1096907 (-) 471 WP_165889003.1 pilin Machinery gene
  A4G16_RS05280 (A4G16_05170) radA 1097093..1098472 (+) 1380 WP_165889004.1 DNA repair protein RadA -
  A4G16_RS05285 (A4G16_05175) - 1098540..1098773 (-) 234 WP_165889005.1 YgjV family protein -
  A4G16_RS05290 (A4G16_05180) folA 1098902..1099390 (-) 489 WP_165889006.1 type 3 dihydrofolate reductase -
  A4G16_RS05295 (A4G16_05185) proB 1099505..1100596 (+) 1092 WP_165889007.1 glutamate 5-kinase -
  A4G16_RS05300 (A4G16_05190) - 1100596..1101357 (+) 762 WP_165889008.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52980.80 Da        Isoelectric Point: 6.0671

>NTDB_id=176301 A4G16_RS05270 WP_165889002.1 1095020..1096414(-) (pilB) [Mannheimia granulomatis strain B 234/94]
MEYVVTDLNSQVLFEISAEQWQKNCNDKQILLRYLAVPLQEDENILWLAIDDMQNLSACEIFAFIAHKQIEPVLISSDEL
KFLLNALSPEQNIQHREMYEGKAEYYSLNENEQHDIHDPIIQILDALFKFCLRKNASDIHLEPQKEKLRVRLRIDGVLHL
YKTFSQSLSSRIISRIKLLAKLDISESRLPQDGQFHFKTQLAETLDFRVSTLPTHWGEKIVLRLQKNKPTHFDFLDLGFS
ESQKNTLLNALQQPQGLILVTGPTGSGKSITLYSALRYLNSNEKHILTAEDPIEIEIEGVIQTQVNRGIELDFSQLLRTF
LRQDPDVIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAFERLLQLGIKEYELKNSLLLVVAQRLLRRLCVKCCG
AGCEHCYQGYKGRIGVYQLFSRTAKNFEKMTACLDFESLQESAKEKLAAGKTNQAEILRVLGNA

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=176301 A4G16_RS05270 WP_165889002.1 1095020..1096414(-) (pilB) [Mannheimia granulomatis strain B 234/94]
ATGGAATATGTTGTAACCGATTTAAATAGCCAAGTGTTATTTGAAATCTCGGCAGAACAATGGCAGAAAAATTGCAATGA
TAAGCAAATTTTATTGCGATATTTAGCTGTGCCTTTGCAGGAAGATGAAAATATATTATGGCTGGCAATTGATGATATGC
AAAATCTTAGCGCTTGTGAGATCTTTGCTTTTATTGCACATAAACAAATTGAACCTGTTTTGATTTCCTCTGATGAATTA
AAATTTTTACTTAATGCTCTTTCTCCTGAGCAGAATATACAACACAGAGAAATGTATGAGGGAAAAGCGGAATATTACAG
CTTAAATGAAAATGAGCAACACGATATTCATGACCCGATCATCCAAATTTTAGATGCCCTTTTTAAATTCTGTTTACGGA
AAAATGCCTCAGATATTCATTTAGAGCCACAAAAAGAGAAATTACGTGTGAGGTTGCGTATTGATGGGGTTTTGCATCTG
TATAAGACTTTTTCACAAAGTTTATCCAGCCGCATTATTTCACGTATTAAATTATTGGCAAAACTAGATATCAGTGAAAG
TCGACTGCCACAAGACGGACAATTTCATTTTAAAACACAGTTGGCGGAAACCTTAGATTTTCGTGTTTCCACACTACCTA
CCCATTGGGGTGAAAAAATCGTCCTCAGATTACAGAAAAATAAACCTACTCATTTTGATTTTCTGGATCTCGGTTTTAGC
GAAAGCCAAAAAAACACCTTACTGAACGCATTACAGCAGCCACAAGGTTTAATTTTAGTTACCGGCCCAACGGGGAGCGG
TAAAAGTATCACGCTTTATAGTGCTTTGAGATATTTAAACAGTAATGAAAAGCATATTTTAACAGCAGAAGATCCGATTG
AAATTGAAATCGAGGGGGTTATTCAGACCCAAGTTAACCGTGGGATTGAGCTGGATTTTAGCCAGCTTTTACGCACATTT
CTCCGACAAGATCCGGATGTCATTATGCTAGGAGAAATTCGCGATGAGGAGAGTGCAGAAATTGCTCTTAGAGCGGCACA
AACTGGGCATTTAGTGCTTTCAACCTTACATACCAATGATGCTCCTTCAGCCTTTGAACGTTTATTGCAGTTAGGCATAA
AAGAGTATGAATTAAAAAATTCACTTTTGTTAGTGGTGGCACAACGTTTATTACGACGACTTTGTGTGAAATGTTGTGGA
GCGGGCTGTGAACATTGTTATCAAGGATATAAAGGCAGAATTGGCGTTTACCAACTGTTTAGTCGAACGGCAAAAAATTT
TGAAAAAATGACCGCTTGTTTAGATTTCGAAAGTTTGCAGGAAAGTGCAAAGGAGAAATTAGCAGCAGGAAAGACCAATC
AAGCTGAAATTTTAAGGGTATTGGGGAATGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(132-393)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8JI73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.8

99.353

0.713
  pilB Haemophilus influenzae Rd KW20

57.792

99.569

0.575
  pilB Haemophilus influenzae 86-028NP

57.235

99.784

0.571
  pilB Vibrio parahaemolyticus RIMD 2210633

39.839

100

0.427
  pilB Vibrio campbellii strain DS40M4

40.619

100

0.425
  pilB Vibrio cholerae strain A1552

40.871

100

0.425
  pilB Acinetobacter baylyi ADP1

37.323

100

0.397
  pilB Acinetobacter baumannii D1279779

36.64

100

0.39
  pilB Legionella pneumophila strain ERS1305867

40.455

94.828

0.384

Multiple sequence alignment