Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A4G16_RS05265 Genome accession   NZ_CP015030
Coordinates   1093831..1095027 (-) Length   398 a.a.
NCBI ID   WP_165889001.1    Uniprot ID   A0A6G8JHY0
Organism   Mannheimia granulomatis strain B 234/94     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1088831..1100027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G16_RS05230 (A4G16_05120) lptE 1090082..1090576 (+) 495 WP_165888994.1 LPS assembly lipoprotein LptE -
  A4G16_RS05235 (A4G16_05125) holA 1090619..1091644 (+) 1026 WP_165888995.1 DNA polymerase III subunit delta -
  A4G16_RS05240 (A4G16_05130) yacG 1091646..1091831 (-) 186 WP_165888996.1 DNA gyrase inhibitor YacG -
  A4G16_RS05245 (A4G16_05135) coaE 1091816..1092451 (-) 636 WP_165888997.1 dephospho-CoA kinase -
  A4G16_RS05250 (A4G16_05140) - 1092460..1092771 (-) 312 WP_165888998.1 helix-turn-helix domain-containing protein -
  A4G16_RS05255 (A4G16_05145) - 1092772..1093149 (-) 378 WP_165888999.1 type II toxin-antitoxin system RelE/ParE family toxin -
  A4G16_RS05260 (A4G16_05150) - 1093192..1093827 (-) 636 WP_165889000.1 prepilin peptidase -
  A4G16_RS05265 (A4G16_05155) pilC 1093831..1095027 (-) 1197 WP_165889001.1 type II secretion system F family protein Machinery gene
  A4G16_RS05270 (A4G16_05160) pilB 1095020..1096414 (-) 1395 WP_165889002.1 GspE/PulE family protein Machinery gene
  A4G16_RS05275 (A4G16_05165) pilA 1096437..1096907 (-) 471 WP_165889003.1 pilin Machinery gene
  A4G16_RS05280 (A4G16_05170) radA 1097093..1098472 (+) 1380 WP_165889004.1 DNA repair protein RadA -
  A4G16_RS05285 (A4G16_05175) - 1098540..1098773 (-) 234 WP_165889005.1 YgjV family protein -
  A4G16_RS05290 (A4G16_05180) folA 1098902..1099390 (-) 489 WP_165889006.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45739.52 Da        Isoelectric Point: 9.8542

>NTDB_id=176300 A4G16_RS05265 WP_165889001.1 1093831..1095027(-) (pilC) [Mannheimia granulomatis strain B 234/94]
MLKIYEYHWKAVDRFQQKRYGKQLADSRIALENYLIAKGYHQIRISRNFVLSQNPKKEDVSQFLSQLALLVNSAIPLKQA
LSMILQNCHNIRLYQWLAELIQFIEHGYTFSQSLQKLDKFITNQEIQLIRMGEQSGKLSIILTNLAKSRMKSERLAKKVK
KILFYPVIVLVISLALSIGLLVFIVPQFAELYGSKAKSLPFITDVLFGLSRFLLEHKISLLVLLLLAVIFFLFFANKLKF
WGLLKMRLLSAMPIFSKIIAHSRIIFFSQNLALMLNAHIPLEMALNSFLSEKSDDPILQYEVQFMLDLLKQGYRFSEGIN
PTVFGSDLVQMIDIGEKSGNLAKMGEHISEIYQHKLDYQIDILSQLLEPVLMLIMGVIVGTIIIGLYLPIFDMGNLME

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=176300 A4G16_RS05265 WP_165889001.1 1093831..1095027(-) (pilC) [Mannheimia granulomatis strain B 234/94]
ATGCTTAAAATTTATGAATACCACTGGAAAGCCGTTGATCGTTTTCAGCAAAAACGTTACGGCAAGCAACTGGCAGATAG
TCGGATAGCGTTAGAAAATTACCTGATTGCTAAGGGATATCATCAAATTCGTATCAGTCGTAATTTTGTGCTGTCGCAAA
ATCCAAAAAAGGAAGATGTAAGTCAGTTTTTATCGCAACTTGCTTTATTGGTTAATTCCGCTATTCCGCTTAAACAAGCG
TTATCTATGATTTTACAAAATTGCCATAATATCCGGCTTTATCAATGGCTGGCTGAGTTAATTCAATTCATTGAACATGG
CTATACTTTTTCCCAATCGCTACAAAAGTTAGATAAATTTATTACGAACCAAGAAATCCAATTGATAAGAATGGGGGAGC
AGAGTGGGAAGCTAAGTATAATTTTGACAAATTTAGCTAAAAGTCGAATGAAATCAGAAAGGCTTGCGAAAAAAGTGAAG
AAAATTTTATTTTATCCTGTAATTGTGTTGGTGATTTCACTCGCACTTTCTATCGGTTTATTGGTGTTTATTGTTCCACA
ATTTGCCGAGCTTTACGGCTCAAAGGCAAAAAGCTTACCTTTTATTACAGATGTGCTATTTGGGCTTTCCCGTTTTTTGC
TAGAACATAAAATAAGTTTACTGGTTCTGCTGCTACTAGCGGTTATTTTTTTCCTGTTTTTTGCAAATAAACTTAAATTC
TGGGGGCTACTAAAAATGCGATTACTGTCTGCTATGCCTATATTTAGCAAGATTATTGCACATTCCCGCATTATATTTTT
CAGCCAAAACCTTGCTCTGATGCTCAATGCTCATATTCCGTTGGAAATGGCATTAAATTCATTTTTATCTGAAAAGAGTG
ATGATCCAATTTTACAATATGAAGTTCAATTTATGCTGGATTTATTAAAGCAAGGTTATCGATTTTCAGAAGGCATAAAT
CCAACAGTGTTTGGCTCGGATTTGGTGCAAATGATAGATATTGGTGAGAAAAGCGGTAATTTGGCAAAAATGGGCGAGCA
TATTAGCGAGATTTATCAGCATAAACTAGATTACCAAATAGACATTCTCTCCCAATTGTTAGAGCCTGTGCTGATGTTAA
TTATGGGAGTGATTGTCGGCACTATTATTATCGGGCTGTATCTACCAATTTTTGATATGGGTAATCTCATGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(63-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8JHY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

55.025

100

0.55
  pilC Haemophilus influenzae Rd KW20

41.646

100

0.42
  pilC Haemophilus influenzae 86-028NP

40.648

100

0.41

Multiple sequence alignment