Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A4G16_RS05275 Genome accession   NZ_CP015030
Coordinates   1096437..1096907 (-) Length   156 a.a.
NCBI ID   WP_165889003.1    Uniprot ID   -
Organism   Mannheimia granulomatis strain B 234/94     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1091437..1101907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G16_RS05240 (A4G16_05130) yacG 1091646..1091831 (-) 186 WP_165888996.1 DNA gyrase inhibitor YacG -
  A4G16_RS05245 (A4G16_05135) coaE 1091816..1092451 (-) 636 WP_165888997.1 dephospho-CoA kinase -
  A4G16_RS05250 (A4G16_05140) - 1092460..1092771 (-) 312 WP_165888998.1 helix-turn-helix domain-containing protein -
  A4G16_RS05255 (A4G16_05145) - 1092772..1093149 (-) 378 WP_165888999.1 type II toxin-antitoxin system RelE/ParE family toxin -
  A4G16_RS05260 (A4G16_05150) - 1093192..1093827 (-) 636 WP_165889000.1 prepilin peptidase -
  A4G16_RS05265 (A4G16_05155) pilC 1093831..1095027 (-) 1197 WP_165889001.1 type II secretion system F family protein Machinery gene
  A4G16_RS05270 (A4G16_05160) pilB 1095020..1096414 (-) 1395 WP_165889002.1 GspE/PulE family protein Machinery gene
  A4G16_RS05275 (A4G16_05165) pilA 1096437..1096907 (-) 471 WP_165889003.1 pilin Machinery gene
  A4G16_RS05280 (A4G16_05170) radA 1097093..1098472 (+) 1380 WP_165889004.1 DNA repair protein RadA -
  A4G16_RS05285 (A4G16_05175) - 1098540..1098773 (-) 234 WP_165889005.1 YgjV family protein -
  A4G16_RS05290 (A4G16_05180) folA 1098902..1099390 (-) 489 WP_165889006.1 type 3 dihydrofolate reductase -
  A4G16_RS05295 (A4G16_05185) proB 1099505..1100596 (+) 1092 WP_165889007.1 glutamate 5-kinase -
  A4G16_RS05300 (A4G16_05190) - 1100596..1101357 (+) 762 WP_165889008.1 TSUP family transporter -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16367.67 Da        Isoelectric Point: 8.4518

>NTDB_id=176302 A4G16_RS05275 WP_165889003.1 1096437..1096907(-) (pilA) [Mannheimia granulomatis strain B 234/94]
MQKITLFRPLQPAFTLIELMIVIAIIAMLATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNTGALTNCSGGSNGI
QANKTAISETKYLSSISVTNGVITVTGKGAVNGYGYSLTPSYSNNTISWSASCSGKDTGLFPANFCSAKPQTTPNG

Nucleotide


Download         Length: 471 bp        

>NTDB_id=176302 A4G16_RS05275 WP_165889003.1 1096437..1096907(-) (pilA) [Mannheimia granulomatis strain B 234/94]
ATGCAAAAAATCACACTTTTTCGACCGCTTCAACCTGCGTTTACTTTAATTGAATTGATGATCGTTATTGCGATTATTGC
TATGTTAGCGACTATTGCCATTCCGTCATACAATTCATATACACAGAAGGCTGCGTTATCTGAATTATTGCAGGCTTCAG
CTTCATATAAATCTGATGTCGAACTCTGTATTTATAACACAGGAGCATTGACTAACTGCTCGGGCGGTTCAAATGGTATT
CAAGCGAATAAAACTGCCATCAGTGAAACTAAATATTTGAGTTCTATTAGTGTGACTAATGGAGTCATTACAGTAACAGG
AAAAGGAGCAGTAAATGGATATGGTTATAGTTTAACGCCGAGTTACAGTAATAATACGATTAGTTGGTCTGCAAGTTGTT
CGGGCAAAGATACCGGCTTATTTCCTGCTAATTTCTGTTCTGCTAAGCCACAAACTACACCAAATGGCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

67.55

96.795

0.654
  pilA Haemophilus influenzae 86-028NP

54.667

96.154

0.526
  pilA Haemophilus influenzae Rd KW20

54.795

93.59

0.513

Multiple sequence alignment