Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PA8281_RS25880 Genome accession   NZ_CP015002
Coordinates   5557234..5558451 (+) Length   405 a.a.
NCBI ID   WP_010792214.1    Uniprot ID   A0A643EEA0
Organism   Pseudomonas aeruginosa strain PA8281     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5552234..5563451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA8281_RS25860 (PA8281_02570) nadC 5553825..5554673 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PA8281_RS25870 (PA8281_02571) - 5554826..5555299 (-) 474 WP_019727264.1 pilin -
  PA8281_RS25875 (PA8281_02572) pilB 5555530..5557230 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PA8281_RS25880 (PA8281_02573) pilC 5557234..5558451 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  PA8281_RS25885 (PA8281_02574) pilD 5558454..5559326 (+) 873 WP_019726811.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PA8281_RS25890 (PA8281_02575) coaE 5559323..5559934 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PA8281_RS25895 (PA8281_02576) yacG 5559931..5560131 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PA8281_RS25900 (PA8281_02577) - 5560168..5560377 (-) 210 WP_003120946.1 hypothetical protein -
  PA8281_RS25905 (PA8281_02578) - 5560483..5561172 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PA8281_RS25910 (PA8281_02579) - 5561169..5561639 (-) 471 WP_019486073.1 hypothetical protein -
  PA8281_RS25915 (PA8281_02580) - 5561636..5562061 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PA8281_RS25920 (PA8281_02581) - 5562194..5562823 (+) 630 WP_019726812.1 DUF1780 domain-containing protein -
  PA8281_RS25925 (PA8281_02582) - 5562820..5563269 (+) 450 WP_003161439.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44242.19 Da        Isoelectric Point: 9.8988

>NTDB_id=175960 PA8281_RS25880 WP_010792214.1 5557234..5558451(+) (pilC) [Pseudomonas aeruginosa strain PA8281]
MADKALKTCVFVWEGTDKKGAKVKGELAGQNTMLVKAQLRKQGINPLKVRKKGITLLGKGKRVKPMDIALFTRQMATMMG
AGVPLLQSFDIISEGFDNPNMRKLVDEIKQEVSAGNSLANSLRKKPLYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVVLVAIIVSAILLIKVVPQFQSVFSSFGAELPAFTMMVINLSNLLQEWWLVVLIGLFSASFAIK
ESHKRSVNFRNTVDRYMLKIPIIGGILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVSKIKQDVSTGM
QLNFSMRTTNVFPNMAIQMTAIGEESGSLDDMLGKVAAFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=175960 PA8281_RS25880 WP_010792214.1 5557234..5558451(+) (pilC) [Pseudomonas aeruginosa strain PA8281]
ATGGCGGATAAAGCGTTAAAAACCTGTGTTTTCGTCTGGGAAGGCACGGACAAGAAAGGGGCAAAGGTCAAGGGGGAGTT
GGCTGGCCAGAACACTATGCTGGTGAAGGCGCAACTGCGCAAACAGGGCATAAATCCGCTCAAGGTCAGAAAAAAGGGCA
TTACCCTTCTCGGCAAGGGAAAGCGCGTAAAGCCGATGGACATCGCACTATTTACTCGTCAGATGGCAACCATGATGGGT
GCTGGGGTTCCTCTCTTGCAATCGTTCGACATAATTTCCGAAGGTTTCGACAATCCCAACATGCGTAAGCTGGTCGACGA
GATCAAACAAGAGGTCTCGGCGGGTAACAGCCTGGCAAACTCCTTGCGAAAGAAACCTCTTTATTTTGACGATCTCTATT
GCAACCTGGTGGATGCAGGTGAACAATCTGGCGCCTTGGAGACTTTGCTTGACCGTGTTGCCACCTATAAGGAAAAAACC
GAATCGCTAAAAGCCAAGATAAAAAAGGCTATGACATACCCCATTGCCGTCGTCTTGGTCGCGATCATTGTTTCAGCCAT
TCTCCTGATCAAGGTCGTACCCCAGTTCCAATCCGTTTTCTCCAGTTTCGGAGCGGAACTACCAGCCTTTACAATGATGG
TGATAAACCTGTCAAATCTTTTACAGGAATGGTGGCTAGTCGTCCTCATCGGATTATTCAGCGCCAGCTTCGCCATCAAG
GAATCGCATAAGCGTTCAGTAAATTTCCGTAACACGGTAGACCGCTATATGTTGAAAATACCGATAATCGGAGGAATACT
TTACAAATCAGCCGTGGCACGTTACGCCCGTACACTTTCAACAACTTTCGCCGCAGGTGTTCCATTGGTGGAGGCACTAG
ACTCTGTCTCTGGAGCGACAGGAAACGTGGTATTCCGCAACGCGGTAAGCAAGATCAAACAGGATGTATCCACAGGTATG
CAATTGAACTTCTCCATGAGAACGACCAATGTATTCCCCAACATGGCAATCCAGATGACAGCCATCGGTGAGGAATCCGG
TTCTCTGGACGACATGTTGGGCAAGGTGGCTGCGTTCTATGAGGAGGAAGTAGACAATGCCGTCGACAATCTGACAACTC
TCATGGAACCTATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A643EEA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baumannii D1279779

58.911

99.753

0.588
  pilC Acinetobacter baylyi ADP1

59.045

98.272

0.58
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria meningitidis 44/76-A

44.03

99.259

0.437
  pilG Neisseria gonorrhoeae MS11

43.781

99.259

0.435
  pilC Vibrio cholerae strain A1552

43.21

100

0.432
  pilC Vibrio campbellii strain DS40M4

43.182

97.778

0.422
  pilC Thermus thermophilus HB27

37.406

99.012

0.37

Multiple sequence alignment