Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OB07_RS23970 Genome accession   NZ_CP014948
Coordinates   5172613..5173830 (+) Length   405 a.a.
NCBI ID   WP_034038724.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain N17-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5167613..5178830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OB07_RS23945 (OB07_04795) nadC 5167714..5168562 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OB07_RS23955 (OB07_04797) - 5168743..5170056 (-) 1314 WP_228845953.1 O-antigen ligase family protein -
  OB07_RS23960 (OB07_04798) - 5170214..5170678 (-) 465 WP_023090842.1 pilin -
  OB07_RS23965 (OB07_04799) pilB 5170909..5172609 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OB07_RS23970 (OB07_04800) pilC 5172613..5173830 (+) 1218 WP_034038724.1 type II secretion system F family protein Machinery gene
  OB07_RS23975 (OB07_04801) pilD 5173831..5174703 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  OB07_RS23980 (OB07_04802) coaE 5174700..5175311 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  OB07_RS23985 (OB07_04803) yacG 5175308..5175508 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  OB07_RS23990 (OB07_04804) - 5175545..5175754 (-) 210 WP_003094660.1 hypothetical protein -
  OB07_RS23995 (OB07_04805) - 5175860..5176549 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  OB07_RS24000 (OB07_04806) - 5176546..5177016 (-) 471 WP_019486073.1 hypothetical protein -
  OB07_RS24005 (OB07_04807) - 5177013..5177438 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  OB07_RS24010 (OB07_04808) - 5177571..5178200 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  OB07_RS24015 (OB07_04809) - 5178197..5178646 (+) 450 WP_023084741.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43979.76 Da        Isoelectric Point: 9.7136

>NTDB_id=175328 OB07_RS23970 WP_034038724.1 5172613..5173830(+) (pilC) [Pseudomonas aeruginosa strain N17-1]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKHSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=175328 OB07_RS23970 WP_034038724.1 5172613..5173830(+) (pilC) [Pseudomonas aeruginosa strain N17-1]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCATTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.519

100

0.785
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment