Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A3Q34_RS07285 Genome accession   NZ_CP014944
Coordinates   1721184..1722434 (+) Length   416 a.a.
NCBI ID   WP_070374763.1    Uniprot ID   A0A1D8RU77
Organism   Colwellia sp. PAMC 20917     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1716184..1727434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q34_RS07260 (A3Q34_07260) ampD 1716486..1717079 (-) 594 WP_231907446.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A3Q34_RS07265 (A3Q34_07265) - 1717122..1717640 (+) 519 WP_070374760.1 retropepsin-like aspartic protease -
  A3Q34_RS07270 (A3Q34_07270) nadC 1717721..1718608 (+) 888 WP_157470874.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A3Q34_RS21075 (A3Q34_07275) - 1718769..1719215 (+) 447 WP_070374761.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A3Q34_RS07280 (A3Q34_07280) pilB 1719396..1721099 (+) 1704 WP_070374762.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3Q34_RS07285 (A3Q34_07285) pilC 1721184..1722434 (+) 1251 WP_070374763.1 type II secretion system F family protein Machinery gene
  A3Q34_RS07290 (A3Q34_07290) pilD 1722503..1723384 (+) 882 WP_231907447.1 A24 family peptidase Machinery gene
  A3Q34_RS07295 (A3Q34_07295) coaE 1723395..1724009 (+) 615 WP_070377048.1 dephospho-CoA kinase -
  A3Q34_RS07300 (A3Q34_07300) zapD 1724117..1724863 (+) 747 WP_070374765.1 cell division protein ZapD -
  A3Q34_RS07305 (A3Q34_07305) yacG 1724976..1725212 (+) 237 WP_070374766.1 DNA gyrase inhibitor YacG -
  A3Q34_RS07310 (A3Q34_07310) mutT 1725215..1725607 (-) 393 WP_070374767.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 416 a.a.        Molecular weight: 45188.17 Da        Isoelectric Point: 9.3288

>NTDB_id=175250 A3Q34_RS07285 WP_070374763.1 1721184..1722434(+) (pilC) [Colwellia sp. PAMC 20917]
MATSGAQKRKLPQAKALDTFVWQGVNRKGKKVNGELPATSMLELKSQLRKQGITPGKVKKKAKSIFGNANDKKIIPADIA
IVTRQIATMLGAGVPLVQTVEMIGKGHSNGNMQRLLGDINNKLQSGIPLSECLRDHPQYFDDLYCDLVSSGEQSGALETI
YDRIATYKEKAEALKSKIKKAMTYPISVLVIAFIVTSILLIFVVPVFQDIFASFGAELPAFTLFVIAISEFMQAYWYLGL
AGLFIASFFFKKAHLNSQSFRDRVDRIILKIPVIGALLEKAAVARYARTLSTTFAAGVPLPDALESAAGASGNAVFRIAI
LEIRAEVSSGMQMNLAMRNCNIFPDMVIQMVAIGEESGAVDDMLAKVATIYEQQVDDAVDSLTSLLEPMIMAVLGVVIGG
LIIAMYLPIFEIGKVV

Nucleotide


Download         Length: 1251 bp        

>NTDB_id=175250 A3Q34_RS07285 WP_070374763.1 1721184..1722434(+) (pilC) [Colwellia sp. PAMC 20917]
ATGGCAACTTCAGGGGCTCAAAAAAGAAAATTACCGCAAGCTAAAGCTCTTGATACGTTTGTTTGGCAAGGGGTAAACCG
TAAGGGTAAAAAAGTTAATGGTGAGCTACCCGCGACCAGCATGCTAGAGCTAAAATCTCAGCTACGCAAACAAGGCATTA
CCCCAGGGAAAGTAAAAAAGAAAGCCAAATCAATTTTTGGGAATGCCAATGACAAAAAAATAATACCTGCAGATATTGCC
ATTGTTACACGGCAAATTGCGACCATGCTTGGCGCTGGTGTCCCCTTAGTACAAACCGTTGAAATGATTGGTAAAGGTCA
TAGCAATGGCAATATGCAAAGATTGTTAGGTGATATCAATAATAAACTGCAATCGGGTATTCCTTTATCAGAATGTTTAC
GCGACCATCCACAATACTTTGATGACTTATATTGTGATTTAGTCAGTTCTGGCGAACAATCGGGCGCACTTGAGACCATT
TATGATCGTATTGCGACTTACAAAGAAAAAGCCGAAGCATTAAAGTCTAAAATTAAAAAAGCGATGACTTATCCTATTTC
TGTATTAGTGATAGCATTTATTGTTACCTCAATATTATTAATTTTTGTCGTGCCGGTATTTCAAGATATTTTTGCCAGCT
TTGGCGCTGAGTTACCTGCGTTTACTTTATTTGTTATTGCCATATCAGAATTTATGCAAGCTTATTGGTATTTAGGCTTA
GCGGGGCTATTTATTGCCAGTTTCTTTTTTAAAAAAGCTCATCTTAACAGCCAAAGTTTTAGAGACCGAGTTGATAGAAT
AATATTAAAAATTCCGGTTATTGGCGCCTTACTCGAAAAGGCAGCAGTTGCCCGCTACGCGAGAACTTTATCTACCACCT
TTGCCGCCGGTGTTCCATTACCTGACGCATTAGAGTCGGCTGCGGGTGCGTCTGGTAATGCGGTTTTTCGTATAGCTATC
CTTGAAATTCGCGCCGAAGTGTCATCGGGTATGCAAATGAATTTAGCAATGCGTAACTGTAATATCTTTCCTGACATGGT
TATACAAATGGTCGCCATTGGTGAAGAATCAGGCGCTGTAGATGATATGCTTGCCAAAGTTGCCACAATATATGAGCAAC
AAGTTGATGACGCGGTAGACTCACTGACCAGTTTATTAGAACCGATGATAATGGCAGTACTCGGCGTAGTGATTGGCGGA
TTAATTATCGCTATGTACTTACCTATTTTCGAAATAGGTAAGGTTGTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(83-205)

Type II secretion system (T2SS), protein F (T2SSF)

(287-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8RU77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.713

95.433

0.589
  pilC Acinetobacter baylyi ADP1

54.455

97.115

0.529
  pilC Acinetobacter baumannii D1279779

54.455

97.115

0.529
  pilC Legionella pneumophila strain ERS1305867

53.98

96.635

0.522
  pilC Vibrio cholerae strain A1552

44.226

97.837

0.433
  pilG Neisseria meningitidis 44/76-A

42.574

97.115

0.413
  pilG Neisseria gonorrhoeae MS11

42.327

97.115

0.411
  pilC Vibrio campbellii strain DS40M4

42.118

97.596

0.411

Multiple sequence alignment