Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A3Q34_RS07280 Genome accession   NZ_CP014944
Coordinates   1719396..1721099 (+) Length   567 a.a.
NCBI ID   WP_070374762.1    Uniprot ID   A0A1D8RU78
Organism   Colwellia sp. PAMC 20917     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1714396..1726099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q34_RS07255 (A3Q34_07255) ampE 1715452..1716372 (-) 921 WP_070374759.1 beta-lactamase regulator AmpE -
  A3Q34_RS07260 (A3Q34_07260) ampD 1716486..1717079 (-) 594 WP_231907446.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A3Q34_RS07265 (A3Q34_07265) - 1717122..1717640 (+) 519 WP_070374760.1 retropepsin-like aspartic protease -
  A3Q34_RS07270 (A3Q34_07270) nadC 1717721..1718608 (+) 888 WP_157470874.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A3Q34_RS21075 (A3Q34_07275) - 1718769..1719215 (+) 447 WP_070374761.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A3Q34_RS07280 (A3Q34_07280) pilB 1719396..1721099 (+) 1704 WP_070374762.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3Q34_RS07285 (A3Q34_07285) pilC 1721184..1722434 (+) 1251 WP_070374763.1 type II secretion system F family protein Machinery gene
  A3Q34_RS07290 (A3Q34_07290) pilD 1722503..1723384 (+) 882 WP_231907447.1 A24 family peptidase Machinery gene
  A3Q34_RS07295 (A3Q34_07295) coaE 1723395..1724009 (+) 615 WP_070377048.1 dephospho-CoA kinase -
  A3Q34_RS07300 (A3Q34_07300) zapD 1724117..1724863 (+) 747 WP_070374765.1 cell division protein ZapD -
  A3Q34_RS07305 (A3Q34_07305) yacG 1724976..1725212 (+) 237 WP_070374766.1 DNA gyrase inhibitor YacG -
  A3Q34_RS07310 (A3Q34_07310) mutT 1725215..1725607 (-) 393 WP_070374767.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 63014.46 Da        Isoelectric Point: 5.2467

>NTDB_id=175249 A3Q34_RS07280 WP_070374762.1 1719396..1721099(+) (pilB) [Colwellia sp. PAMC 20917]
MKGHHRQLSVLSTLLKHNIVAENKVDIISADFLKQSKPFIRFLIEDKSLDAENIAKILSRSFGYPLIDLNAFDTAMIPDG
IRNEKLIRKHNALPLFLRGKVLFVAMSDPTDLDALEDIQFNTGFPTELVLTNDKDLQIFIDKILEEESDALDITDIDAEE
LAGIDVQEERQEDDSATGDKEDAPIVVYINKILLDAIRKGASDLHFEPYEKSYRIRFRVDGILTEMAKPPVSLASRMAAR
LKVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKVVMRILDSSSAMLGIEMLGYEPAQKQIYMDALAQPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLDGVNQVQINVRAGLTFPNALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSASETLTRLLNMGVPSYNVASSVSIIIAQRLARRLCNRCKVEDVIPRPELEKQGFT
PEQLSEIKLFKPVGCDQCTGGYKGRVGVYEVIKISEKMSSIIMEGGNSMDIAQQCQKEGYNNLRQSGLEKAHQGVTSLTE
INRITSA

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=175249 A3Q34_RS07280 WP_070374762.1 1719396..1721099(+) (pilB) [Colwellia sp. PAMC 20917]
ATGAAAGGACATCACAGACAATTAAGTGTGTTATCTACACTTTTAAAACATAATATTGTCGCCGAAAATAAGGTTGACAT
TATAAGTGCTGACTTCCTTAAACAATCAAAACCTTTCATTCGTTTTCTTATTGAAGATAAGAGTTTAGACGCTGAAAATA
TTGCTAAAATACTCTCTCGCAGTTTTGGCTATCCTCTCATAGATTTAAACGCTTTTGACACGGCTATGATCCCCGATGGT
ATCCGTAACGAAAAATTGATCCGCAAACATAATGCATTGCCGCTATTTCTTAGAGGTAAAGTATTATTCGTAGCGATGTC
TGATCCCACTGATTTAGATGCATTAGAAGATATTCAGTTCAACACCGGTTTTCCTACCGAATTAGTGTTGACTAATGATA
AAGATTTACAAATATTTATTGACAAAATTCTTGAAGAAGAAAGTGATGCTCTAGATATTACCGATATTGATGCTGAAGAA
CTCGCAGGTATTGACGTTCAAGAAGAGCGTCAAGAAGATGATAGCGCTACCGGTGATAAAGAAGATGCACCGATTGTTGT
CTATATCAATAAGATATTACTCGATGCTATTCGCAAAGGGGCTTCAGATTTACACTTTGAACCTTACGAAAAATCATACC
GTATACGCTTTCGTGTTGATGGTATTTTAACTGAGATGGCAAAGCCGCCCGTATCACTTGCCTCTCGTATGGCAGCACGT
TTAAAAGTCATGTCAAAGCTCGATATCGCTGAACGGCGAGTCCCCCAAGATGGACGGATAAAATTAGCCTTGTCCAAGAA
AAAATCAATTGATTTTCGTGTTTCAACGTTACCGACGATGTGGGGCGAAAAAGTGGTAATGCGTATTCTTGACTCTTCAA
GTGCGATGTTAGGTATTGAAATGCTCGGCTATGAGCCGGCGCAAAAGCAAATTTACATGGACGCTTTAGCGCAACCACAA
GGTATGATATTAGTCACGGGACCAACCGGCTCAGGTAAAACAGTGTCTCTTTACACCGGGTTAAATATTCTTAACACCGA
AGAACGTAATATATCAACAGCAGAAGATCCGGTAGAAATAAATTTAGACGGCGTTAATCAAGTACAAATTAATGTGCGTG
CCGGCTTAACCTTTCCTAATGCGCTGCGCTCTTTTCTTCGCCAAGATCCCGATATTGTTATGGTAGGGGAAATTCGTGAT
CTTGAAACTGCAGAAATAGCGATTAAAGCCGCGCAAACCGGTCACTTAGTGTTGTCCACATTGCACACAAACTCAGCCTC
AGAAACCCTGACTCGATTATTAAACATGGGCGTTCCTTCATATAACGTGGCCAGTTCGGTCAGTATTATTATTGCTCAGC
GTTTAGCCAGACGCTTATGTAATCGCTGTAAAGTTGAGGATGTTATACCTAGACCTGAACTAGAAAAGCAAGGTTTTACC
CCTGAGCAACTTAGCGAAATAAAATTATTTAAACCCGTAGGTTGCGATCAATGTACGGGCGGCTATAAAGGTCGTGTTGG
TGTTTATGAAGTGATAAAAATCAGTGAAAAAATGTCTTCTATCATTATGGAAGGCGGAAATTCTATGGATATCGCTCAGC
AGTGTCAAAAAGAAGGTTACAATAACTTACGCCAATCGGGTTTAGAAAAAGCCCATCAAGGGGTAACAAGTTTGACAGAA
ATAAACCGTATAACGTCTGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8RU78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.094

98.06

0.57
  pilB Acinetobacter baylyi ADP1

59.484

95.767

0.57
  pilB Legionella pneumophila strain ERS1305867

57.627

93.651

0.54
  pilB Vibrio cholerae strain A1552

53.686

93.298

0.501
  pilB Vibrio campbellii strain DS40M4

52.562

92.945

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

52.264

93.474

0.489
  pilF Neisseria gonorrhoeae MS11

50.093

95.062

0.476
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.552

94.533

0.374

Multiple sequence alignment