Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A3Q33_RS05870 Genome accession   NZ_CP014943
Coordinates   1404390..1405646 (+) Length   418 a.a.
NCBI ID   WP_081179143.1    Uniprot ID   -
Organism   Colwellia sp. PAMC 21821     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1399390..1410646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q33_RS05845 (A3Q33_05835) ampD 1399453..1400034 (-) 582 WP_081179140.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A3Q33_RS05850 (A3Q33_05840) - 1400176..1400697 (+) 522 WP_081148344.1 retropepsin-like aspartic protease -
  A3Q33_RS05855 (A3Q33_05845) nadC 1400698..1401585 (+) 888 WP_081179141.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A3Q33_RS05860 (A3Q33_05850) - 1401924..1402370 (+) 447 WP_286160900.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A3Q33_RS05865 (A3Q33_05855) pilB 1402500..1404206 (+) 1707 WP_081179142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3Q33_RS05870 (A3Q33_05860) pilC 1404390..1405646 (+) 1257 WP_081179143.1 type II secretion system F family protein Machinery gene
  A3Q33_RS05875 (A3Q33_05865) pilD 1405853..1406713 (+) 861 WP_081179144.1 A24 family peptidase Machinery gene
  A3Q33_RS05880 (A3Q33_05870) coaE 1406841..1407452 (+) 612 WP_081179145.1 dephospho-CoA kinase -
  A3Q33_RS05885 (A3Q33_05875) zapD 1407545..1408291 (+) 747 WP_081179146.1 cell division protein ZapD -
  A3Q33_RS05890 (A3Q33_05880) yacG 1408472..1408708 (+) 237 WP_081179147.1 DNA gyrase inhibitor YacG -
  A3Q33_RS05895 (A3Q33_05885) mutT 1408805..1409203 (-) 399 WP_081179148.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45409.69 Da        Isoelectric Point: 9.5165

>NTDB_id=175225 A3Q33_RS05870 WP_081179143.1 1404390..1405646(+) (pilC) [Colwellia sp. PAMC 21821]
MAVSSTIKVKDKKSKVKALDVYKWQGLNRKGKKISGELNATSILELKAQLRKQGITPGKISKKAKPLFGMSGDKKILPVD
IAILTRQIATMLGAGVPLVQTIEMIGSGHNNANMQKLLSTIGNKLQSGIPLSECLREHPKYFDDLYCDLVNSGEQSGSLE
TIYDRIAIYKEKAEVLKAKIKKAMTYPISVLIIALIVTSVLLIFVVPVFKDIFDSFGAELPGFTLFVLAISDFMQSYWYF
GLAGIFITGYLFKKAHANSLNFRDSVDKKILKLPVVGDVLKKAAVARYARTLSTTFAAGVPLPDALESAAGASGNAVFRD
AILEIRAEVTSGMQMNLAMRNCAIFPDMVIQMVAIGEESGAVDDMLAKVATVYEQEVDNAVDNLTTLLEPMIMAVLGVVI
GGLIVAMYLPIFQIGMVV

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=175225 A3Q33_RS05870 WP_081179143.1 1404390..1405646(+) (pilC) [Colwellia sp. PAMC 21821]
ATGGCTGTTTCTTCAACAATAAAAGTAAAAGACAAGAAAAGTAAAGTTAAAGCACTTGATGTTTATAAATGGCAAGGGCT
GAACCGTAAAGGTAAAAAAATTAGTGGCGAGCTTAATGCCACCAGTATATTAGAACTTAAAGCCCAGCTGCGCAAGCAAG
GTATAACCCCAGGTAAAATCAGCAAAAAAGCTAAACCATTATTTGGTATGAGCGGCGATAAAAAAATTCTCCCGGTTGAT
ATCGCGATACTAACTCGACAAATTGCCACTATGCTTGGCGCAGGTGTGCCTTTAGTACAAACCATTGAAATGATAGGTTC
TGGTCACAACAATGCCAATATGCAAAAATTACTCAGTACCATTGGGAATAAACTGCAATCAGGTATCCCGCTTTCTGAAT
GCTTACGTGAACACCCTAAATATTTTGATGATTTATATTGCGACTTGGTTAACTCTGGTGAGCAATCAGGTTCACTAGAA
ACCATATACGATCGTATTGCTATCTATAAAGAAAAAGCCGAAGTATTAAAAGCCAAAATTAAAAAAGCCATGACCTACCC
TATTTCGGTCTTAATTATCGCCTTAATTGTTACATCGGTCTTATTAATTTTTGTTGTACCTGTTTTTAAAGATATTTTTG
ATAGCTTTGGCGCAGAATTACCTGGTTTTACTTTATTTGTGCTCGCTATATCAGACTTTATGCAGTCTTATTGGTATTTT
GGTCTTGCCGGTATATTTATTACGGGTTACCTCTTTAAAAAGGCGCATGCCAATAGCCTTAATTTCCGTGATAGCGTCGA
TAAAAAAATATTAAAATTACCTGTTGTTGGTGATGTATTAAAAAAAGCGGCGGTTGCCCGTTATGCCAGAACGCTATCAA
CTACTTTTGCTGCCGGCGTACCTTTACCTGATGCATTAGAGTCAGCGGCTGGCGCTTCTGGCAATGCGGTGTTTCGTGAT
GCTATTTTAGAAATACGAGCTGAAGTTACCTCAGGTATGCAAATGAATTTAGCCATGCGTAATTGTGCCATTTTCCCAGA
TATGGTTATTCAAATGGTGGCGATTGGTGAAGAGTCAGGCGCAGTTGACGATATGCTAGCCAAAGTAGCTACGGTATATG
AGCAAGAAGTAGATAACGCCGTAGATAACTTAACTACCCTGCTAGAGCCTATGATTATGGCTGTGTTAGGTGTGGTTATT
GGCGGTTTAATTGTTGCCATGTATTTACCTATTTTCCAAATTGGTATGGTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(289-410)

Type II secretion system (T2SS), protein F (T2SSF)

(85-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.804

95.215

0.579
  pilC Acinetobacter baylyi ADP1

53.695

97.129

0.522
  pilC Acinetobacter baumannii D1279779

52.723

96.651

0.51
  pilC Legionella pneumophila strain ERS1305867

51.707

98.086

0.507
  pilC Vibrio cholerae strain A1552

45.161

96.411

0.435
  pilC Vibrio campbellii strain DS40M4

43.609

95.455

0.416
  pilG Neisseria meningitidis 44/76-A

44.444

92.584

0.411
  pilG Neisseria gonorrhoeae MS11

44.444

92.584

0.411

Multiple sequence alignment