Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A3Q33_RS05865 Genome accession   NZ_CP014943
Coordinates   1402500..1404206 (+) Length   568 a.a.
NCBI ID   WP_081179142.1    Uniprot ID   -
Organism   Colwellia sp. PAMC 21821     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1397500..1409206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3Q33_RS05840 (A3Q33_05830) ampE 1398325..1399230 (-) 906 WP_081179139.1 beta-lactamase regulator AmpE -
  A3Q33_RS05845 (A3Q33_05835) ampD 1399453..1400034 (-) 582 WP_081179140.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A3Q33_RS05850 (A3Q33_05840) - 1400176..1400697 (+) 522 WP_081148344.1 retropepsin-like aspartic protease -
  A3Q33_RS05855 (A3Q33_05845) nadC 1400698..1401585 (+) 888 WP_081179141.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A3Q33_RS05860 (A3Q33_05850) - 1401924..1402370 (+) 447 WP_286160900.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A3Q33_RS05865 (A3Q33_05855) pilB 1402500..1404206 (+) 1707 WP_081179142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3Q33_RS05870 (A3Q33_05860) pilC 1404390..1405646 (+) 1257 WP_081179143.1 type II secretion system F family protein Machinery gene
  A3Q33_RS05875 (A3Q33_05865) pilD 1405853..1406713 (+) 861 WP_081179144.1 A24 family peptidase Machinery gene
  A3Q33_RS05880 (A3Q33_05870) coaE 1406841..1407452 (+) 612 WP_081179145.1 dephospho-CoA kinase -
  A3Q33_RS05885 (A3Q33_05875) zapD 1407545..1408291 (+) 747 WP_081179146.1 cell division protein ZapD -
  A3Q33_RS05890 (A3Q33_05880) yacG 1408472..1408708 (+) 237 WP_081179147.1 DNA gyrase inhibitor YacG -
  A3Q33_RS05895 (A3Q33_05885) mutT 1408805..1409203 (-) 399 WP_081179148.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62333.57 Da        Isoelectric Point: 5.0690

>NTDB_id=175224 A3Q33_RS05865 WP_081179142.1 1402500..1404206(+) (pilB) [Colwellia sp. PAMC 21821]
MKGLHQQSSLLSALTKHNIVPSDKAEKISAGFIQQNKPFIRYLVEDKSINADDIASTLCRSFGCPLIDLANFDTSLVPEG
VRNEKLIRKHHALPLYLRGKVLFVAMSDPTNLDALEEIQFNTGFSTELVIANETALAACIEKILEEDSDALDISDMDAEE
LSGIDVQDERKEEDNIGGGDKDDAPIVVYINKILLDAIRKGASDLHFEPYEKSYRIRFRIDGILNEVAKPPVSLASRMAA
RLKVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKVVMRILDSSSAMLGIDMLGYAPEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLDGINQVQINVRAGLTFPGALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVTIIIAQRLARRLCVQCREEEHIPEAELLAQGF
LPAQLENIKLFKPVGCDLCTGGYKGRVGIYEVIKISDVTANLIMTGSNSLDIAAQCQREGYNNLRQSGLEKAALGVTSLE
EVNRVTNA

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=175224 A3Q33_RS05865 WP_081179142.1 1402500..1404206(+) (pilB) [Colwellia sp. PAMC 21821]
ATGAAAGGACTTCATCAACAATCAAGCCTGCTTTCCGCCTTAACTAAGCATAATATTGTGCCTAGTGATAAAGCAGAAAA
AATTAGTGCAGGTTTTATTCAACAAAATAAGCCTTTTATTCGCTATTTAGTTGAAGATAAAAGCATTAATGCTGATGACA
TTGCAAGCACGCTCTGTCGAAGTTTTGGCTGTCCGCTAATAGACTTAGCTAATTTTGATACTAGCTTAGTGCCTGAAGGT
GTACGTAACGAAAAACTGATCCGTAAACACCATGCCTTACCGTTATATTTACGTGGCAAAGTGTTATTCGTTGCCATGTC
GGACCCGACTAATCTTGATGCGCTTGAAGAAATTCAATTTAATACCGGTTTTTCTACTGAGTTGGTTATTGCCAACGAAA
CCGCTTTAGCTGCCTGTATTGAAAAAATATTAGAGGAAGATAGCGATGCCCTTGATATTTCAGACATGGACGCGGAAGAA
TTATCGGGTATAGATGTTCAAGATGAGCGCAAAGAAGAAGATAACATTGGTGGCGGTGATAAAGATGATGCCCCGATTGT
TGTTTATATTAATAAAATTTTACTCGACGCGATTCGCAAAGGCGCGTCTGATTTACATTTTGAACCTTACGAGAAGTCTT
ACCGTATTCGTTTTCGTATTGACGGTATTCTTAACGAAGTTGCCAAACCACCTGTATCGCTCGCCTCGCGCATGGCGGCA
CGTTTAAAAGTTATGTCGAAGTTAGATATTGCTGAACGCCGCGTGCCACAAGATGGCCGTATAAAATTAGCTTTATCGAA
AAAGAAGTCGATTGATTTTCGTGTAAGTACGCTGCCTACCATGTGGGGCGAAAAAGTAGTAATGCGTATTCTTGACTCGT
CAAGTGCCATGCTAGGTATCGATATGCTGGGCTACGCGCCAGAGCAAAAGAAAATTTACATGGACGCGCTAGCGCAACCT
CAAGGTATGATTTTAGTGACCGGCCCCACGGGTTCAGGTAAAACTGTTTCGCTTTATACCGGGCTTAATATTTTAAATAC
CGAAGAGCGTAATATTTCAACCGCGGAAGATCCGGTTGAAATTAACCTCGACGGTATTAACCAAGTACAAATTAATGTCC
GCGCTGGTTTAACCTTCCCTGGCGCATTACGCTCATTCCTTCGACAAGATCCCGATATTGTTATGGTTGGTGAGATCCGT
GATTTAGAAACCGCAGAAATTGCCATTAAAGCCGCGCAAACGGGTCATTTAGTGTTATCAACATTGCATACTAACTCGGC
AGCCGAAACACTTACTCGATTATTGAATATGGGCGTACCCTCATACAACGTTGCCAGTTCAGTAACTATTATTATTGCTC
AACGACTCGCAAGGCGTTTATGTGTTCAATGTCGAGAAGAAGAGCATATCCCTGAAGCCGAACTGCTCGCGCAAGGTTTT
TTACCTGCACAACTAGAGAACATTAAATTATTTAAACCTGTAGGCTGCGATTTGTGTACCGGCGGCTACAAAGGCCGTGT
CGGTATATATGAAGTCATAAAAATTAGCGATGTTACCGCAAACTTAATTATGACCGGCTCGAACTCGTTAGATATTGCTG
CACAATGCCAACGCGAAGGCTACAATAATTTGCGCCAGTCTGGCTTAGAAAAAGCCGCTCTAGGGGTAACCAGTTTAGAA
GAAGTAAATCGCGTTACCAACGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.322

93.486

0.555
  pilB Legionella pneumophila strain ERS1305867

57.248

95.951

0.549
  pilB Acinetobacter baumannii D1279779

58.868

93.31

0.549
  pilB Vibrio cholerae strain A1552

54.269

92.782

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

52.174

93.134

0.486
  pilF Neisseria gonorrhoeae MS11

48.577

98.944

0.481
  pilB Vibrio campbellii strain DS40M4

51.901

92.606

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

41.2

88.028

0.363

Multiple sequence alignment