Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A3218_RS17270 Genome accession   NZ_CP014867
Coordinates   3919007..3920224 (-) Length   405 a.a.
NCBI ID   WP_062824570.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain 189 isolate 189     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3914007..3925224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3218_RS17240 (A3218_17240) - 3915870..3916340 (+) 471 WP_062824566.1 hypothetical protein -
  A3218_RS17245 (A3218_17245) - 3916337..3917026 (+) 690 WP_009045672.1 energy-coupling factor ABC transporter permease -
  A3218_RS17250 (A3218_17250) - 3917092..3917307 (+) 216 WP_062824567.1 hypothetical protein -
  A3218_RS17255 (A3218_17255) yacG 3917312..3917515 (-) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  A3218_RS17260 (A3218_17260) coaE 3917512..3918135 (-) 624 WP_062824568.1 dephospho-CoA kinase -
  A3218_RS17265 (A3218_17265) pilD 3918132..3919004 (-) 873 WP_062824569.1 A24 family peptidase Machinery gene
  A3218_RS17270 (A3218_17270) pilC 3919007..3920224 (-) 1218 WP_062824570.1 type II secretion system F family protein Machinery gene
  A3218_RS17275 (A3218_17275) - 3920588..3921004 (+) 417 WP_007924673.1 pilin -
  A3218_RS17280 (A3218_17280) - 3921290..3921646 (+) 357 WP_062824571.1 BON domain-containing protein -
  A3218_RS17285 (A3218_17285) - 3921849..3924767 (-) 2919 WP_062824572.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44467.35 Da        Isoelectric Point: 10.5241

>NTDB_id=174890 A3218_RS17270 WP_062824570.1 3919007..3920224(-) (pilC) [Pseudomonas chlororaphis strain 189 isolate 189]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKPSSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTSALRKRPRHFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HARRRSPRFRHWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=174890 A3218_RS17270 WP_062824570.1 3919007..3920224(-) (pilC) [Pseudomonas chlororaphis strain 189 isolate 189]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCACTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAACCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAACTGGCGACCTTGATCAAG
GCCGGCGTACCACTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCGCTGCGCAAGAGGCCCCGGCACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGGATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCGGTGTTCACGCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCGCTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCGCCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCACTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGAGTGCCGCTGGTGGAGGCGCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGTGCATCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.938

100

0.649
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.637

100

0.56
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

41.542

99.259

0.412
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilG Neisseria meningitidis 44/76-A

40.796

99.259

0.405
  pilC Vibrio campbellii strain DS40M4

39.055

99.259

0.388

Multiple sequence alignment