Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   BFL21_RS14950 Genome accession   NZ_CP018243
Coordinates   2660672..2662096 (+) Length   474 a.a.
NCBI ID   WP_000132546.1    Uniprot ID   -
Organism   Escherichia coli strain 350     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2655672..2667096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL21_RS14910 (BFL21_14765) - 2655839..2656054 (+) 216 WP_000188870.1 hypothetical protein -
  BFL21_RS14915 (BFL21_14770) - 2656153..2656374 (+) 222 WP_000763383.1 TraR/DksA family transcriptional regulator -
  BFL21_RS14920 (BFL21_14775) - 2656371..2657318 (+) 948 WP_001289930.1 ead/Ea22-like family protein -
  BFL21_RS31095 - 2657320..2657498 (+) 179 Protein_2650 hypothetical protein -
  BFL21_RS31525 - 2657827..2658183 (+) 357 Protein_2651 hypothetical protein -
  BFL21_RS14930 (BFL21_14785) - 2658180..2658530 (+) 351 WP_000610373.1 DUF551 domain-containing protein -
  BFL21_RS14935 (BFL21_14790) - 2658718..2659062 (+) 345 WP_001281188.1 hypothetical protein -
  BFL21_RS14940 (BFL21_14795) - 2659140..2659331 (+) 192 WP_000132739.1 AlpA family phage regulatory protein -
  BFL21_RS14945 (BFL21_14800) - 2659312..2660490 (-) 1179 WP_001007946.1 site-specific integrase -
  BFL21_RS14950 (BFL21_14805) sbcB 2660672..2662096 (+) 1425 WP_000132546.1 exodeoxyribonuclease I Machinery gene
  BFL21_RS14955 (BFL21_14810) tsuB 2662139..2662366 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  BFL21_RS14960 (BFL21_14815) tsuA 2662380..2663438 (-) 1059 WP_000492327.1 thiosulfate utilization transporter TsuA/YeeE -
  BFL21_RS14965 (BFL21_14820) plaP 2663617..2664975 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  BFL21_RS31100 yoeI 2664965..2665027 (-) 63 WP_010723108.1 membrane protein YoeI -
  BFL21_RS14970 (BFL21_14825) yeeY 2665242..2666171 (-) 930 WP_000803334.1 LysR substrate-binding domain-containing protein -
  BFL21_RS14975 (BFL21_14830) yeeZ 2666217..2667041 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54397.63 Da        Isoelectric Point: 5.2440

>NTDB_id=174387 BFL21_RS14950 WP_000132546.1 2660672..2662096(+) (sbcB) [Escherichia coli strain 350]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=174387 BFL21_RS14950 WP_000132546.1 2660672..2662096(+) (sbcB) [Escherichia coli strain 350]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAACGAA
GCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGCTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAACCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGATAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAACTGGTACATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584

Multiple sequence alignment