Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AC731_RS10850 Genome accession   NZ_CP014646
Coordinates   2309509..2311221 (+) Length   570 a.a.
NCBI ID   WP_048705994.1    Uniprot ID   A0A127K605
Organism   Thauera humireducens strain SgZ-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2304509..2316221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC731_RS10825 (AC731_010825) - 2304824..2305606 (-) 783 WP_004261067.1 M48 family metallopeptidase -
  AC731_RS10830 (AC731_010830) - 2305603..2306352 (-) 750 WP_048705988.1 competence/damage-inducible protein A -
  AC731_RS10835 (AC731_010835) - 2306349..2307125 (-) 777 WP_048705991.1 EI24 domain-containing protein -
  AC731_RS10840 (AC731_010840) ffh 2307139..2308509 (-) 1371 WP_004261078.1 signal recognition particle protein -
  AC731_RS10845 (AC731_010845) - 2308561..2309391 (+) 831 WP_038011754.1 cytochrome C assembly family protein -
  AC731_RS10850 (AC731_010850) pilB 2309509..2311221 (+) 1713 WP_048705994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AC731_RS10855 (AC731_010855) pilC 2311242..2312471 (+) 1230 WP_038011757.1 type II secretion system F family protein Machinery gene
  AC731_RS10860 (AC731_010860) pilD 2312475..2313326 (+) 852 WP_048705996.1 prepilin peptidase Machinery gene
  AC731_RS10865 (AC731_010865) - 2313473..2313736 (+) 264 WP_048705998.1 FmdB family zinc ribbon protein -
  AC731_RS10870 (AC731_010870) - 2313794..2314417 (+) 624 WP_038011779.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 61988.07 Da        Isoelectric Point: 5.2332

>NTDB_id=172966 AC731_RS10850 WP_048705994.1 2309509..2311221(+) (pilB) [Thauera humireducens strain SgZ-1]
MAANPQPALSGFARALIQHGRLSEADALACTAQASDGTNAFILEVAARGLMSTAAIARFAADTFGYPLLDIAAFDKSMFA
SDAVDRKLMHKHQALALAKRQNRVTLAIADPSNMRALDEIRFQTGMQLDLVIAEADKLKRVADAQNESTEQTLKELTGDE
FDMDLLKEEAPSEHKEEDTSDVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLVLKEKIA
ARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYEPDQRKALMEAVER
PYGMILVTGPTGSGKTVSLYTCLNILNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFSAALRAFLRQDPDVIMVGEI
RDLDTAEISVKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKPADIPLEALLEAGF
SPDELDGSWQPYAPGGCDRCKGSGYKGRVGIYQVMPITEEIAHIIMTGGNSMDIAAQAESEGVRDLRKSGLLKVKQGVTS
LEEVLATTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=172966 AC731_RS10850 WP_048705994.1 2309509..2311221(+) (pilB) [Thauera humireducens strain SgZ-1]
ATGGCGGCGAATCCCCAACCTGCCCTGAGCGGTTTCGCTCGGGCCCTCATCCAGCATGGACGGCTGTCCGAAGCAGACGC
ACTCGCGTGTACCGCGCAGGCCAGCGACGGCACCAATGCATTCATCCTGGAGGTCGCGGCACGCGGCCTGATGTCGACGG
CGGCCATCGCACGTTTTGCGGCCGACACTTTCGGCTACCCCCTGCTCGACATCGCCGCATTCGACAAGTCGATGTTCGCC
AGCGATGCGGTCGACCGCAAGCTGATGCACAAGCACCAGGCGCTCGCGCTCGCCAAGCGCCAGAACCGTGTCACGCTTGC
CATCGCCGATCCCTCCAACATGCGCGCGCTGGACGAGATCCGCTTCCAGACCGGCATGCAGCTCGACCTCGTGATCGCCG
AGGCCGACAAGCTCAAGCGCGTTGCCGATGCACAGAACGAGTCGACCGAGCAGACCCTGAAGGAGCTGACGGGCGACGAG
TTCGACATGGACCTGCTCAAGGAAGAGGCGCCTTCGGAGCACAAGGAAGAGGACACCAGCGACGTCGACGACGCTCCGGT
GGTCAAGTTCATCCAGAAGGTGCTGATCGACGCGATCAATGAAGGTGCGTCCGACATCCACTTCGAACCTTACGAGAAGT
ATTACCGCATCCGGGTACGGACCGACGGCGTGCTGCGTGAGATCGCGCAGCCGCCGCTGGTACTGAAGGAAAAGATCGCC
GCACGGATCAAGGTCATCTCGCGCCTCGACATTTCGGAGAAACGTGTCCCGCAAGACGGACGCATGAAGCTCGTGCTGTC
GAAGAACAAGGCGATCGACTTCCGTGTCTCGACGCTTCCGACCCTGCACGGCGAGAAGATCGTGATGCGTATTCTCGACC
CGAGTTCGGCGATGCTCGGTATCGATGCCCTGGGTTACGAGCCTGACCAGCGCAAGGCGCTGATGGAAGCGGTCGAGCGC
CCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTCTACACCTGCCTCAATATCCTCAA
CAAGGCGGGCGTCAATATCTCGACCGCGGAAGATCCGGCCGAAATCAACCTGCCCGGCATCAACCAGGTCAACGTCAACG
AAAAGGCCGGCCTCACCTTCTCGGCGGCGCTGCGTGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTGGGTGAAATC
CGCGACCTCGACACGGCCGAGATCTCGGTCAAGGCGGCGCAGACGGGCCACCTGGTGCTGTCGACCCTGCACACCAACGA
CGCACCGACCACCCTCGAGCGCCTCAAGAACATGGGTGTCGCGCCGTTCAACATCGCGTCATCGGTGATACTGATCACGG
CCCAGCGTCTGGCGCGCCGGTTATGCTCGTGCAAGAAGCCGGCCGACATACCGCTCGAAGCCCTGCTCGAGGCGGGCTTC
AGCCCGGATGAACTCGATGGCAGCTGGCAGCCCTACGCGCCCGGCGGCTGCGACCGCTGCAAGGGCAGCGGGTACAAGGG
CCGCGTGGGTATTTACCAGGTGATGCCTATTACGGAAGAGATTGCGCATATCATAATGACCGGTGGCAATTCGATGGACA
TTGCCGCCCAGGCAGAAAGCGAGGGCGTGCGCGACCTGCGCAAGTCCGGCCTGCTCAAGGTCAAACAAGGCGTGACCTCG
CTAGAGGAAGTGCTGGCGACAACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-142)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127K605

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.098

100

0.565
  pilB Acinetobacter baylyi ADP1

55.477

99.298

0.551
  pilB Legionella pneumophila strain ERS1305867

52.557

99.474

0.523
  pilF Neisseria gonorrhoeae MS11

51.865

98.772

0.512
  pilB Vibrio cholerae strain A1552

53.057

88.947

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

49.522

91.754

0.454
  pilB Vibrio campbellii strain DS40M4

48.952

92.105

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

40.691

91.404

0.372
  pilF Thermus thermophilus HB27

39.773

92.632

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.81

92.105

0.367

Multiple sequence alignment