Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AC731_RS10860 Genome accession   NZ_CP014646
Coordinates   2312475..2313326 (+) Length   283 a.a.
NCBI ID   WP_048705996.1    Uniprot ID   A0A127K647
Organism   Thauera humireducens strain SgZ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2307475..2318326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC731_RS10845 (AC731_010845) - 2308561..2309391 (+) 831 WP_038011754.1 cytochrome C assembly family protein -
  AC731_RS10850 (AC731_010850) pilB 2309509..2311221 (+) 1713 WP_048705994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AC731_RS10855 (AC731_010855) pilC 2311242..2312471 (+) 1230 WP_038011757.1 type II secretion system F family protein Machinery gene
  AC731_RS10860 (AC731_010860) pilD 2312475..2313326 (+) 852 WP_048705996.1 prepilin peptidase Machinery gene
  AC731_RS10865 (AC731_010865) - 2313473..2313736 (+) 264 WP_048705998.1 FmdB family zinc ribbon protein -
  AC731_RS10870 (AC731_010870) - 2313794..2314417 (+) 624 WP_038011779.1 DUF502 domain-containing protein -
  AC731_RS10875 (AC731_010875) aspS 2314486..2316285 (+) 1800 WP_048706000.1 aspartate--tRNA ligase -
  AC731_RS10880 (AC731_010880) - 2316468..2318117 (+) 1650 WP_048706002.1 response regulator -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30742.44 Da        Isoelectric Point: 7.0924

>NTDB_id=172968 AC731_RS10860 WP_048705996.1 2312475..2313326(+) (pilD) [Thauera humireducens strain SgZ-1]
MLALLQDPLAFTGVGALLGLFVGSFLNVVIHRLPRMMERDWHVQAAELRGEEVQPTERFNLATPRSRCPHCGHLIGALEN
IPVLSYLVLRGRCGHCGARISLRYPIVEAFTALLSGYAAWHFGFGLAAAGALLFIWTMVALAFIDIDTQLLPDDLTLPLL
WLGLLFNLGDTFTDLPSAVIGAMAGYLALWSVYWLFKLVTGKEGMGYGDFKLLAAIGAWLGWSLLPLTILLSSLVGAVVG
ITLIVLARHGRNVPIPFGPYLAAAGIIALFWGETLTSRYLGLL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=172968 AC731_RS10860 WP_048705996.1 2312475..2313326(+) (pilD) [Thauera humireducens strain SgZ-1]
ATGCTGGCGCTCTTGCAAGACCCGCTCGCCTTCACCGGCGTGGGCGCCCTGCTGGGATTGTTCGTCGGCAGCTTCCTGAA
CGTCGTGATCCACCGCCTGCCAAGGATGATGGAGCGTGACTGGCATGTACAAGCTGCCGAGTTGAGGGGCGAGGAGGTCC
AGCCGACCGAGCGCTTCAATCTCGCGACGCCGCGGTCGCGCTGCCCGCACTGCGGGCACCTCATCGGCGCGCTCGAGAAC
ATCCCGGTCCTGAGCTACCTCGTGCTGCGGGGGCGCTGCGGCCACTGCGGCGCCCGGATCAGCCTGCGCTACCCGATCGT
CGAGGCGTTCACCGCCCTGCTCTCGGGCTACGCTGCCTGGCATTTCGGTTTCGGGCTCGCGGCTGCCGGCGCCCTGCTGT
TCATCTGGACGATGGTGGCGCTGGCCTTCATCGACATCGATACGCAGCTGCTGCCCGACGATCTGACGCTTCCTCTGCTT
TGGCTTGGCCTGCTCTTCAACCTGGGCGACACCTTTACCGATCTGCCCAGTGCAGTGATCGGCGCCATGGCAGGGTATCT
TGCGTTGTGGTCAGTGTATTGGCTGTTCAAGCTCGTCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGCTGCTGG
CAGCCATTGGCGCCTGGCTGGGCTGGTCGCTGCTGCCGCTGACGATCCTGCTCTCGTCGCTCGTCGGCGCGGTCGTCGGC
ATCACCCTCATCGTCCTTGCCCGCCACGGTCGCAACGTGCCGATCCCCTTTGGCCCCTACCTGGCCGCAGCGGGCATCAT
CGCCCTGTTCTGGGGCGAGACCCTGACGTCCCGCTACCTCGGTCTGCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(133-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127K647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.072

97.527

0.537
  pilD Vibrio campbellii strain DS40M4

52.174

97.527

0.509
  pilD Acinetobacter baumannii D1279779

50.714

98.94

0.502
  pilD Acinetobacter nosocomialis M2

50.357

98.94

0.498
  pilD Neisseria gonorrhoeae MS11

50.391

90.459

0.456

Multiple sequence alignment