Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AC731_RS10855 Genome accession   NZ_CP014646
Coordinates   2311242..2312471 (+) Length   409 a.a.
NCBI ID   WP_038011757.1    Uniprot ID   A0A127K602
Organism   Thauera humireducens strain SgZ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2306242..2317471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC731_RS10835 (AC731_010835) - 2306349..2307125 (-) 777 WP_048705991.1 EI24 domain-containing protein -
  AC731_RS10840 (AC731_010840) ffh 2307139..2308509 (-) 1371 WP_004261078.1 signal recognition particle protein -
  AC731_RS10845 (AC731_010845) - 2308561..2309391 (+) 831 WP_038011754.1 cytochrome C assembly family protein -
  AC731_RS10850 (AC731_010850) pilB 2309509..2311221 (+) 1713 WP_048705994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AC731_RS10855 (AC731_010855) pilC 2311242..2312471 (+) 1230 WP_038011757.1 type II secretion system F family protein Machinery gene
  AC731_RS10860 (AC731_010860) pilD 2312475..2313326 (+) 852 WP_048705996.1 prepilin peptidase Machinery gene
  AC731_RS10865 (AC731_010865) - 2313473..2313736 (+) 264 WP_048705998.1 FmdB family zinc ribbon protein -
  AC731_RS10870 (AC731_010870) - 2313794..2314417 (+) 624 WP_038011779.1 DUF502 domain-containing protein -
  AC731_RS10875 (AC731_010875) aspS 2314486..2316285 (+) 1800 WP_048706000.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44150.15 Da        Isoelectric Point: 9.9581

>NTDB_id=172967 AC731_RS10855 WP_038011757.1 2311242..2312471(+) (pilC) [Thauera humireducens strain SgZ-1]
MATASRAMRPAGPQEDLYNWEGKDKSGKIVRGEIRASGDAVVKAMLRRQGILVTKVKKQKLSRGGKISEKDIALFTRQLA
TMMKSGVPLLQAFDIGIKGSGNPALARLLNEVRNDVETGSSLSQAFAKHPVHFDRLFTNLVAAGEQAGILDSLLDRIATY
KEKILAIKGKIKSALFYPIAVVVVAALVISVMMLFVIPEFKSVFSGFGAELPAPTMIVIGISDFFVEFWYIVLGIPIVAI
MAISWIYKRSVAMQIAIDRLVLKLPVVGDIIRKATVARWTRTLSTMFAAGVPLVEALDSVGGASGNHIYLTATRQIQSDV
STGTSLTVAMQNANVFPTMVVQMVSIGEESGQLDAMLGKVADFFEQEVDEAVSGLSQLLEPIIMVFLGTVIGGLVIAMYL
PIFKLGSIV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=172967 AC731_RS10855 WP_038011757.1 2311242..2312471(+) (pilC) [Thauera humireducens strain SgZ-1]
ATGGCAACGGCTAGCCGCGCAATGCGCCCCGCAGGCCCCCAGGAGGATCTGTACAACTGGGAAGGCAAGGACAAGTCCGG
CAAGATCGTGCGCGGCGAGATCAGGGCTTCCGGTGACGCGGTCGTCAAGGCCATGCTCCGCCGCCAGGGCATCCTGGTCA
CCAAGGTCAAGAAACAGAAGCTGTCGCGTGGCGGCAAGATCAGCGAGAAGGACATCGCGCTCTTCACCCGCCAGCTGGCG
ACAATGATGAAATCCGGCGTGCCGCTGCTTCAGGCCTTCGACATCGGCATCAAGGGTTCTGGCAACCCTGCGCTGGCACG
CCTGCTGAACGAAGTCCGGAACGACGTCGAAACCGGCTCGAGCCTGTCGCAGGCCTTCGCCAAGCACCCCGTGCATTTCG
ATCGTCTGTTCACCAACCTGGTCGCCGCCGGCGAACAGGCCGGCATCCTGGACAGCCTGCTGGACCGGATCGCCACCTAC
AAGGAAAAGATCCTTGCCATAAAGGGCAAGATCAAGTCGGCGCTGTTCTATCCGATCGCGGTCGTCGTGGTGGCCGCACT
GGTGATCTCGGTGATGATGCTGTTCGTCATTCCCGAGTTCAAGAGTGTCTTCTCCGGGTTCGGAGCCGAACTGCCGGCGC
CGACCATGATCGTCATCGGAATTTCCGACTTCTTTGTCGAGTTCTGGTACATCGTGCTCGGCATCCCCATTGTCGCGATC
ATGGCCATCAGCTGGATCTACAAACGCTCCGTCGCCATGCAGATCGCGATCGATCGCCTCGTACTCAAGCTGCCCGTCGT
CGGCGACATCATCCGCAAGGCGACGGTGGCACGCTGGACCCGTACGCTGTCGACCATGTTCGCAGCCGGCGTCCCCCTTG
TCGAAGCGCTGGATTCGGTCGGCGGCGCCTCCGGCAACCACATCTATCTGACTGCGACCCGCCAGATCCAGAGCGACGTG
AGCACCGGCACGAGCCTGACCGTCGCCATGCAGAACGCCAACGTGTTCCCGACCATGGTGGTGCAGATGGTGTCGATCGG
CGAGGAGTCCGGTCAGCTGGATGCCATGCTCGGCAAGGTGGCGGACTTCTTCGAGCAGGAAGTCGACGAGGCCGTATCCG
GGCTGTCGCAGCTGCTCGAGCCGATCATCATGGTCTTCCTCGGCACCGTTATCGGCGGCCTGGTGATCGCGATGTATCTT
CCGATCTTCAAGCTCGGCTCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127K602

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.632

97.555

0.513
  pilC Legionella pneumophila strain ERS1305867

52.764

97.311

0.513
  pilG Neisseria gonorrhoeae MS11

50.126

97.066

0.487
  pilG Neisseria meningitidis 44/76-A

49.874

97.066

0.484
  pilC Acinetobacter baylyi ADP1

48.492

97.311

0.472
  pilC Acinetobacter baumannii D1279779

47.859

97.066

0.465
  pilC Vibrio campbellii strain DS40M4

41.523

99.511

0.413
  pilC Vibrio cholerae strain A1552

41.667

96.822

0.403
  pilC Thermus thermophilus HB27

39.152

98.044

0.384

Multiple sequence alignment