Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VVCECT4999_RS03155 Genome accession   NZ_CP014636
Coordinates   622225..623451 (-) Length   408 a.a.
NCBI ID   WP_039448924.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 617225..628451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS03115 (VVCECT4999_03080) rpsP 617415..617663 (+) 249 WP_011079541.1 30S ribosomal protein S16 -
  VVCECT4999_RS03120 (VVCECT4999_03085) rimM 617679..618227 (+) 549 WP_038941427.1 ribosome maturation factor RimM -
  VVCECT4999_RS03125 (VVCECT4999_03090) trmD 618255..619004 (+) 750 WP_011079543.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  VVCECT4999_RS03130 (VVCECT4999_03095) rplS 619046..619399 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  VVCECT4999_RS03135 (VVCECT4999_03100) yacG 619623..619817 (-) 195 WP_038941426.1 DNA gyrase inhibitor YacG -
  VVCECT4999_RS03140 (VVCECT4999_03105) zapD 619880..620620 (-) 741 WP_017421189.1 cell division protein ZapD -
  VVCECT4999_RS03145 (VVCECT4999_03110) coaE 620653..621261 (-) 609 WP_039448926.1 dephospho-CoA kinase -
  VVCECT4999_RS03150 (VVCECT4999_03115) pilD 621263..622132 (-) 870 WP_038941424.1 prepilin peptidase Machinery gene
  VVCECT4999_RS03155 (VVCECT4999_03120) pilC 622225..623451 (-) 1227 WP_039448924.1 type II secretion system F family protein Machinery gene
  VVCECT4999_RS03160 (VVCECT4999_03125) pilB 623499..625187 (-) 1689 WP_039448922.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VVCECT4999_RS03165 (VVCECT4999_03130) - 625187..625630 (-) 444 WP_039448920.1 pilin -
  VVCECT4999_RS03170 (VVCECT4999_03135) nadC 625853..626740 (-) 888 WP_039448918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VVCECT4999_RS03175 (VVCECT4999_03140) ampD 626833..627405 (+) 573 WP_039448916.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45240.54 Da        Isoelectric Point: 10.3681

>NTDB_id=172855 VVCECT4999_RS03155 WP_039448924.1 622225..623451(-) (pilC) [Vibrio vulnificus strain CECT 4999]
MKLKTHSQIKNYQWKGINGAGKKVSGNTLALTELEVRDKLKMQHIQIKKIKKSSVSVFTRLSHKVKGKDITLLTRQLATM
LTTGVPILQSLKLVADNHRKAEMKSILASVSKAVEAGTPLSKALRSASPQFDGIYVDLVATGEQSGNLDQVFERLAFYRE
KNEQLRAKVVKALIYPCMVVLVALGVSYLMLTKVIPEFRKMFTGFGAELPWFTQKVLDLSDFVQAYSSWFFITLFSAIYL
LRGLSKKSPAFRLRMSRLSLKIPVIGGVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTAGNLHYQYAIEDVYRDTAA
GMPMYIAMRNSLAFPELVLQMVMIGEESGQMDDMLNKIATIYEFEVDNTVDNLGKILEPLIIVFLGVVVGGLVTAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=172855 VVCECT4999_RS03155 WP_039448924.1 622225..623451(-) (pilC) [Vibrio vulnificus strain CECT 4999]
ATGAAGCTTAAAACCCATTCACAAATCAAAAACTATCAATGGAAAGGGATTAACGGAGCTGGAAAAAAAGTTTCCGGCAA
CACGCTGGCGCTCACCGAGCTTGAAGTGCGTGATAAGCTGAAAATGCAGCATATCCAGATCAAAAAGATCAAAAAATCCA
GTGTGTCAGTTTTCACGAGGTTAAGCCATAAAGTCAAAGGCAAAGACATCACACTGCTTACCCGCCAACTCGCCACCATG
CTGACCACTGGTGTTCCTATTCTACAATCGCTCAAATTGGTTGCCGACAACCATCGCAAAGCTGAGATGAAATCAATCTT
AGCCAGTGTGAGCAAAGCCGTTGAAGCAGGCACGCCGTTATCCAAAGCGCTTCGCAGTGCCAGCCCACAATTCGATGGCA
TTTATGTTGACTTAGTCGCCACTGGTGAACAATCGGGTAATCTTGATCAAGTTTTTGAACGCCTCGCTTTTTATCGGGAA
AAAAACGAGCAGTTGCGAGCAAAAGTGGTCAAGGCATTGATTTATCCCTGCATGGTCGTGCTGGTCGCGTTGGGGGTCTC
TTATTTAATGCTGACCAAGGTTATTCCCGAGTTTCGCAAAATGTTCACCGGATTCGGCGCAGAACTGCCTTGGTTTACGC
AAAAAGTCTTAGACCTGTCTGATTTTGTGCAAGCCTACAGCAGTTGGTTTTTTATTACGTTGTTCAGTGCTATCTATTTA
CTTCGCGGTCTAAGCAAGAAATCACCCGCATTTCGCTTAAGAATGTCACGCTTGAGTTTAAAGATCCCTGTAATTGGTGG
GGTCTTATCCAAAGCAGCGATTGCCAAATTTAGCCGCACATTAGCCACCAGCTTCAGTGCGGGTATCCCGATCCTCACCA
GTTTAAAAACCACCAGTAAAACCGCAGGCAATCTGCATTACCAATATGCGATTGAAGATGTTTATCGTGACACCGCAGCC
GGAATGCCAATGTACATCGCCATGCGTAACAGCCTTGCTTTCCCAGAATTGGTACTGCAAATGGTGATGATTGGTGAAGA
ATCTGGCCAAATGGACGATATGCTCAACAAAATTGCCACCATCTATGAGTTTGAGGTGGATAACACCGTCGACAACCTCG
GTAAGATTCTCGAGCCGTTGATCATCGTCTTTCTAGGCGTTGTCGTCGGCGGATTGGTCACCGCAATGTACCTGCCGATC
TTTAATTTAATGAGCGTTCTGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

78

98.039

0.765
  pilC Vibrio campbellii strain DS40M4

74.813

98.284

0.735
  pilC Pseudomonas stutzeri DSM 10701

41.584

99.02

0.412
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

41.045

98.529

0.404
  pilC Acinetobacter baumannii D1279779

40

100

0.402
  pilC Acinetobacter baylyi ADP1

40.852

97.794

0.4
  pilC Legionella pneumophila strain ERS1305867

40.099

99.02

0.397

Multiple sequence alignment