Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AX767_RS05250 Genome accession   NZ_CP014517
Coordinates   1032195..1033928 (+) Length   577 a.a.
NCBI ID   WP_068629269.1    Uniprot ID   A0A126Z9Z9
Organism   Variovorax sp. PAMC 28711 strain PAMC28711     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1027195..1038928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AX767_RS05215 (AX767_05215) - 1027388..1027894 (+) 507 WP_237288555.1 CNP1-like family protein -
  AX767_RS05220 (AX767_05220) proB 1027900..1029054 (-) 1155 WP_068629259.1 glutamate 5-kinase -
  AX767_RS05225 (AX767_05225) obgE 1029056..1030132 (-) 1077 WP_068629261.1 GTPase ObgE -
  AX767_RS05230 (AX767_05230) rpmA 1030200..1030460 (-) 261 WP_068629263.1 50S ribosomal protein L27 -
  AX767_RS05235 (AX767_05235) rplU 1030473..1030784 (-) 312 WP_068629265.1 50S ribosomal protein L21 -
  AX767_RS05240 (AX767_05240) - 1030964..1031956 (+) 993 WP_068629267.1 polyprenyl synthetase family protein -
  AX767_RS05250 (AX767_05250) pilB 1032195..1033928 (+) 1734 WP_068629269.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AX767_RS05255 (AX767_05255) pilC 1033949..1035169 (+) 1221 WP_068629271.1 type II secretion system F family protein Machinery gene
  AX767_RS05260 (AX767_05260) - 1035169..1036131 (+) 963 WP_068629273.1 A24 family peptidase -
  AX767_RS05265 (AX767_05265) coaE 1036210..1036755 (+) 546 WP_237288556.1 dephospho-CoA kinase -
  AX767_RS05270 (AX767_05270) - 1036838..1038328 (+) 1491 WP_068629278.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62380.54 Da        Isoelectric Point: 5.6111

>NTDB_id=172024 AX767_RS05250 WP_068629269.1 1032195..1033928(+) (pilB) [Variovorax sp. PAMC 28711 strain PAMC28711]
MAAAEPLVKEHSPIALPGLARALIAAGKLPAKAAEEIYQKSQSSRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDPQRLPKDLLDTKLCQAYRIVVLSKRSNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMVEAAAVSAAETI
EGIVGAEFEFDEASADTSGDSNEQAVAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHQYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKAR
LLSAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVVCREPADIPR
EALLDAGYAEADANGSWTPYRPVGCSACNGGYKGRVGIYQVMPITEEIQKIILRDGSALDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVAVTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=172024 AX767_RS05250 WP_068629269.1 1032195..1033928(+) (pilB) [Variovorax sp. PAMC 28711 strain PAMC28711]
ATGGCCGCTGCCGAACCTCTCGTCAAAGAACATTCGCCCATAGCCCTTCCTGGGCTCGCGCGCGCCTTGATAGCGGCAGG
CAAATTGCCCGCAAAGGCGGCCGAGGAGATTTATCAGAAGTCGCAGAGCAGCCGCACCAGTTTCATCGCCGAATTGACGG
GCACCGGTGCCGTGTCTGCAGCCGATCTTGCCCACACACTGTCGAGCGCGTTCGGCGCCCCGCTGCTCGACCTCGATGCG
ATCGACCCGCAGCGGCTCCCGAAGGACCTGCTCGACACCAAGCTCTGTCAGGCGTATCGGATCGTCGTTCTCAGCAAGCG
CAGCAACCGCCTGATCGTTGCAACGGCCGATCCGTCTGACCAGCAAGCGGCAGAGAAGATCAAGTTCGCGTCGCAAATGG
GTGTCGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGCATGGTCGAAGCCGCAGCGGTCAGCGCGGCTGAAACCATC
GAAGGCATCGTCGGTGCCGAGTTCGAGTTCGACGAGGCCTCCGCGGACACGTCGGGCGACTCCAACGAACAGGCCGTCGC
CGAAGTCGAAGACGCGCCAGTCGTCCGGTTCCTGCACAAGATGCTGCTGGACGCATTCAGCATGCGCGCATCGGACATCC
ATTTCGAGCCCTACGAGCACCAGTATCGGGTGCGATTTCGGGTAGACGGCGAGCTGCGCGAGATAGCCAGCCCGCCGACC
GTCATCAAGGACAAGCTCGCGTCCCGCATCAAGGTCATTTCCCGGCTGGATATTTCCGAGAAGCGCGTCCCGCAAGACGG
CCGGATGAAGCTGAAGATCGGCCCCGACCGCGTCATCGATTTTCGGGTGAGCACCCTGCCAACGCTGTTTGGCGAAAAGA
TCGTGATCCGGATTCTCGATCCGAGCAGCGCCCGCCTCGGCATCGATGCGCTCGGCTACGACGCCGATGAAAAGGCCCGA
CTCCTCAGTGCCATCGGGCGGCCGTACGGCATGGTTCTCGTCACGGGTCCCACGGGCTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAATCTCCTGAACAAGCCGGGCGTGAACATCGCCACGGCAGAAGATCCGTCGGAAATCAACTTGCCTGGCG
TGAACCAGGTCAATGTGAATGAGAAGGCGGGCCTGACCTTCGCGACCGCGTTGCGCGCCTTTCTGCGTCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTTGAAACCGCCGACATCTCCATCAAGGCGGCGCAAACAGGTCACCTCGTGCT
GTCCACGCTGCACACGAACGACGCGCCCACCACGCTCACGCGGATGCGCAACATGGGGATTGCGCCCTTCAACATCGCCT
CCAGCGTGATCCTGATCACCGCGCAGCGCTTGGCCCGCCGGCTCTGCGTCGTGTGTCGCGAGCCCGCCGACATTCCGCGG
GAAGCACTGCTCGATGCCGGCTATGCCGAAGCGGACGCCAACGGCTCGTGGACGCCTTACCGCCCGGTGGGATGTTCTGC
CTGCAACGGCGGCTACAAAGGCCGCGTCGGCATCTACCAAGTGATGCCGATCACCGAAGAAATTCAGAAGATCATCCTGC
GCGACGGCAGCGCGCTGGACATCGCAAGGCAGTCCGAGGCGGAGGGTGTTCGATCCCTGCGTCAATCCGGGCTGCGCAAG
GTCATGCAGGGACTCACATCACTCGAAGAAGTGGTCGCGGTCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A126Z9Z9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.321

98.267

0.534
  pilB Acinetobacter baylyi ADP1

54.24

98.094

0.532
  pilF Neisseria gonorrhoeae MS11

52.135

97.4

0.508
  pilB Legionella pneumophila strain ERS1305867

48.763

98.094

0.478
  pilB Vibrio cholerae strain A1552

46.631

97.747

0.456
  pilB Vibrio campbellii strain DS40M4

46.018

97.92

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

44.938

97.574

0.438
  pilF Thermus thermophilus HB27

38.448

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

91.854

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

34.677

100

0.373

Multiple sequence alignment