Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AYJ52_RS06865 Genome accession   NZ_CP014477
Coordinates   1456277..1457989 (-) Length   570 a.a.
NCBI ID   WP_017402590.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AP_882     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1451277..1462989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ52_RS06835 (AYJ52_06835) - 1451440..1451766 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  AYJ52_RS06840 (AYJ52_06840) rlmB 1451869..1452618 (+) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AYJ52_RS06845 (AYJ52_06845) - 1452654..1453568 (+) 915 WP_017402594.1 DMT family transporter -
  AYJ52_RS06850 (AYJ52_06850) coaE 1453565..1454161 (-) 597 WP_017402593.1 dephospho-CoA kinase -
  AYJ52_RS06855 (AYJ52_06855) pilD 1454163..1455023 (-) 861 WP_017402592.1 prepilin peptidase Machinery gene
  AYJ52_RS06860 (AYJ52_06860) pilC 1455023..1456249 (-) 1227 WP_017402591.1 type II secretion system F family protein Machinery gene
  AYJ52_RS06865 (AYJ52_06865) pilB 1456277..1457989 (-) 1713 WP_017402590.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AYJ52_RS06870 (AYJ52_06870) tpiA 1458281..1459075 (+) 795 WP_017402589.1 triose-phosphate isomerase -
  AYJ52_RS06875 (AYJ52_06875) secG 1459088..1459417 (+) 330 WP_017398731.1 preprotein translocase subunit SecG -
  AYJ52_RS06895 (AYJ52_06895) rimP 1460055..1460579 (+) 525 WP_002114922.1 ribosome maturation factor RimP -
  AYJ52_RS06900 (AYJ52_06900) nusA 1460617..1462101 (+) 1485 WP_002115134.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63595.96 Da        Isoelectric Point: 5.0911

>NTDB_id=171846 AYJ52_RS06865 WP_017402590.1 1456277..1457989(-) (pilB) [Acinetobacter pittii strain AP_882]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFNDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=171846 AYJ52_RS06865 WP_017402590.1 1456277..1457989(-) (pilB) [Acinetobacter pittii strain AP_882]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAGGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCAATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAGTTTCGAGTTTAATGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTTCGTTATCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAGAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCTT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCAACACTACATACCAATAGTGC
TCCAGAGACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGGGTTACTTCT
TTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.088

100

0.951
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.323

99.474

0.511
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.958

91.579

0.467
  pilB Vibrio cholerae strain A1552

49.903

90.702

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment